Estimating the enzyme specificity and glucose concentration of sugar solutions

Rishan Singh

Abstract
The function of the human body are controlled by enzymes and hormones. Similarly, in the laboratory, the enzyme, glucose oxidase, is used to hydrolyse glucose. In the human body, glucose and insulin control blood glucose levels, which are dependent on food intake. In this study, the enzyme specificity and glucose concentration of sugar solutions would be estimated. In order to achieve this, an experiment involving the use of indicator strips and a two-fold serial dilution was performed. Furthermore, conclusions on blood glucose and serum insulin levels would also be discussed from a given data set.

Keywords
Enzyme; Reactants products; Potassium iodide; Energy supplement; Food intake; Hormones; Pancreas.

DOI
10.21472/bjbs.061401

Full text
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References
Becker, Wayne M.; Deamer, David W. The world of the cell. San Francisco: Pearson Education, 2003. (Benjamin/Cummings Series in the Life Sciences).

BIOL 103 Practical Manual. Introductory Biology for the Health Sciences. School of Life Sciences, UKZN, Westville, 2019.

Hans, P.; Vanthuyne, A.; Dewandre, P. Y.; Brichant, J. F.; Bonhomme, V. Blood glucose concentration profile after 10 mg dexamethasone in non-diabetic and type 2 diabetic patients undergoing abdominal surgery. BJA: British Journal of Anaesthesia, v. 97, no. 2, p. 164-170, 2006. https://doi.org/10.1093/bja/ael111

Matthews, C. K.; van Holde, K. E.; Ahern, K. G. Biochemistry. San Franscisco: Addison Wesley Longman, 2000.

Singh, R. Personal writing and communic¬ation. Representing the Republic of South Africa, 2019.

Wright, T. E.; Martin, D.; Qualls, C.; Curet, L. B. Effects of intrapartum administration of invert sugar and D5LR on neonatal blood glucose levels. Journal of Perinatology, v. 20, no. 4, p. 217-218, 2000. https://doi.org/10.1038/sj.jp.7200366



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