Isolation and characterization of some flavonoids from the leaf of Tapinanthus globiferus growing on Vitex doniana

H. Abubakar , A. M. Musa , M. I. Abdullahi , V. Mzozoyana and A. J. Yusuf

Abstract
Tapinanthus globiferus (A. Rich) Tiegh (Loranthacae) is a semi-parasitic plant growing on several plant species such as Vitex doniana. it is used in ethno-medicine for the treatment of fungal infection, itching, hypertension, ulcers, epilepsy, diabetes and cancer. The aim of this study was to isolate bioactive compound(s) from the leaf of T. globiferus. The powdered plant material was extracted with 90% methanol using maceration method and the resulting crude methanol leaf extract was partitioned into n-hexane, chloroform, ethylacetate and n-butanol fractions. The ethylacetate fraction was chromatographed on a silica gel and sephadex LH-20 column which led to the isolation of two flavonoids, and the identity of the compounds was determined on the basis of chemical test and NMR analysis. Based on the 1D and 2D NMR data, the compounds were 2-(3'4'-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromane-4-one (quercetin) and 2-(2,4-dihydroxyphenyl)-5,7-dihydroxy-3-(3-methylhexyl)-4H-chromen-4-one. This is the first report of isolation of these compounds from T. globiferus growing on Vitex doniana.

Keywords
Tapinanthus globiferus; Leaf; Isolation; NMR.

DOI
10.21472/bjbs(2020)071701

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References
Abedo, J. A.; Jonah, O. A.; Abdullahi, R. S.; Mazadu, M. R.; Idris, H. Y.; Muhammed, H.; Shettima, F. T.; Ombugadu, S.; Daudu, M.; Garba, J.; Abdulmalik, U.; Kugu, B. A.; Usman, A. O. Evaluation of trypanosomal activity of Tapinanthus globiferus and Gongronema latifolium on Trypanosoma congolense. Bioscience Research, v. 10, p. 2-8, 2013.

Abubakar, K.; Adebisi, I. M.; Ugwah-Oguejiofor, J. C.; Idris, G. O.; Idris, B.; Mshelia, H. E. Phytochemical screening and anticonvulsant activity of the residual aqueous fraction of Tapinanthus globiferus growing on Ficus glums. Herbal Medicine: Open Access, v. 2, no. 2, 7, 2016. https://doi.org/10.21767/2472-0151.100013

Bassey, M. E. Phytochemical investigation of Tapinanthus globiferus from two hosts and the taxonomic implication. International Journal of Chemical Environmental and Pharmaceutical Research, v. 3, no. 2, p. 174-177, 2012.

Burkill, H. M. Useful plants of West Tropical Africa. 2. ed. Kew: Royal Botanic Gardens, 2000. v. 5.

Mabry, T. J.; Markham, K. R.; Thomas, M. B. The systematic identification of flavonoids. New York: Springer-Verlag, 1970.

Ogunbolude, Y.; Ibrahim, M.; Elekofehinti, O. O.; Adeniran, A.; Abolaji, A. O.; Rocha, J. B. T.; Kamdem, J. P. Effects of Tapinanthus globiferus and Zanthoxylum zanthoxyloides extracts on human leukocytes in vitro. Journal of Intercultural Ethnopharmacology, v. 3, no. 4, p. 167-172, 2014. https://doi.org/10.5455/jice.20140826110059

Polhill, R.; Wiens, D. Mistletoe of Africa. Kew: The Royal Botanic Garden, 1998.

Pratkit, C. Chemical constituent from the root bark and leaves of Artocarpus elasticus. Songkhla: Prince of Songkla University, 2010. (M.Sc. thesis).

Sani, Y. M.; Musa, A. M.; Abdullahi, S. M.; Nasir, T.; Abdullahi, M. I.; Atiku, I. Quercetin and ß-sitosterol isolated from the methanol leaves extract of Cissus polyantha Glig and Brandt (Vitaceae). Nigerian Journal of Pharmaceutical Sciences, v. 14, no. 2, p. 46-50, 2015.

Sher, H.; Alyemeni, M. N. Pharmaceutically important plants used in traditional system of Arab medicine for the treatment of livestock ailments in the Kingdom of Saudi Arabia. African Journal of Biotechnology, v. 10, p. 9153-9159, 2011. https://doi.org/10.5897/AJB10.1570

Silva G. L.; Lee, I.; Douglas K. A. Special problems with extraction of plants. In: Cannell, J. P. R. (Ed.). Natural products isolation. New Jersey: Human Publishers, 1998. p. 251-293.

Waterberg, F.; Craven, P.; Marais, L. Common world flowers of the Okavango Delta. Namibia: Gamsberg Publishers, 1989. (Shellfield guide series II).

Yineger, H.; Yewhalaw, D. Traditional medicinal plant knowledge and use by local healers in Sekoru District, Jimma Zone, Southwestern Ethiopia. Journal of Ethnobiology and Ethnomedicine, v. 3:24, 2007. https://doi.org/10.1186/1746-4269-3-24


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