Histopathology and other aspects of echinostome infection in freshwater fishes of the South Western Ghats, India

Puthiyakandy Janardhanan Jithila and Puthanpurayil Kandambeth Prasadan

Abstract
While investigating the trematode parasite infestation in the freshwater fishes of South Western Ghats, India we came across echinostome infection in three species of fishes Pseudosphromenus cupanus, Lepidocephalichthys thermlis and Aplochelus lineatus. Detailed histopathological studies on the infected kidney of the freshwater fish L. thermlis revealed severe damage and degeneration of their cells. The prevalence, intensity and mean abundance of infection of Echinostoma sp. in the three fishes were also recorded. The prevalences of Echinostoma sp. infection in the freshwater fishes P. cupanus, A. lineatus and L. thermalis were 5.55%, 66.66% and 25%, the intensities of infection were 4.5, 36 and 1, and the mean abundances were 0.25, 24 and 0.25, respectively.

Keywords
Echinostoma sp.; freshwater fishes; histopathology; Western Ghats.

DOI
10.21472/bjbs.061312

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References
Bush, A. O.; Lafferty, K. D.; Lotz, J. M.; Shostak, A. W. Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, v. 83, p. 575-583, 1997.

Cantwell, G. E. Methods for invertebrates. In: Clark, G. (Ed.). Staining procedures. Baltimore: Williams and Wilkins, 1981. p. 255-280.

Chai, J. Y.; Hong, S. J.; Sohn, W. M.; Lee, S. H.; Seo, B. S. Studies on intestinal trematodes in Korea. XVI. Infection status of loaches with the metacercariae of Echinostoma hortense. Korean Journal of Parasitology, v. 23, p. 18-23, 1985.

Chai, J. Y.; Hong, S. T.; Lee, S. H.; Lee, G. C.; Min, Y. I. A case of echinostomiasis with ulcerative lesions in the duodenum. Korean Journal of Parasitology, v. 32, p. 201-204, 1994.

Chai, J. Y.; Sohn, W. M.; Yong, T. S.; Eom, K. S.; Min, D. Y.; Hoang, E. H.; Phammasack, B.; Insisiengmay, B.; Rim, H. J. Echinostome flukes recovered from humans in Khammouane Province, Lao PDR. Korean Journal of Parasitology, v. 50, p. 269-272, 2012.

Esteban, J. G.; Muñoz-Antoli, C. Echinostomes: Systematics and life cycles. In: Fried, B.; Toledo, R. (Eds.). The biology of echinostomes. New York: Springer, 2009. https://doi.org/10.1007/978-0-387-09577-6_1

Evans, W. A. The histopathology of cutthroat trout experimentally infected with the blood fluke Sanguinicola klamathensis. Journal of Wildlife Diseases, v. 10, no. 3, p. 243-248, 1974. https://doi.org/10.7589/0090-3558-10.3.243

Fried, B. Biology of echinostomes except echinostoma. Advances in Parasitology, v. 49, p. 163-210, 2001.

Fried, B.; Huffman, J. E.; Weiss, P. M. Single and multiple worm infections of Echinostoma caproni (Trematoda) in the golden hamster. Journal of Helminthology, v. 64, p. 75-78, 1990.

Fried, B.; Pane, P. L.; Reddy, A. Experimental infection of Rana pipiens tadpoles with Echinostoma trivolvis cercariae. Parasitology Research, v. 83, p. 666-669, 1997.

Haseeb, M. A.; Eveland, L. K. Human echinostomasis: Mechanisms of pathogenesis and host resistance. In: Fried, B.; Graczyk, T. K. (Eds.). Echinostomes as models for biological research. Dordrecht: Kluwer, 2002. p. 83-98.

Holland, M. P.; Skelly, D. K.; Kashgarian, M.; Bolden, S. R.; Harrison, L. M.; Cappello, M. Echinostome infection in green frogs (Rana clamitans) is stage and age dependent. Journal of Zoology, v. 271, p. 455-462, 2006. https://doi.org/10.1111/j.1469-7998.2006.00229.x

Huffman, J. E.; Fried, B. Echinostoma and echinostomiasis. Advances in Parasitology, v. 29, p. 215-269, 1990.

Joet, L. K.; Umathevy, T. Studies on Echinostomatidae (Trematoda) in Malaya. XI. The life history of Echinostoma hystricosum sp. n. Journal of Parasitology, v. 52 p. 449-453, 1966.

Jyrwa, D. B.; Thapa, S.; Tandon, V. Helminth parasite spectrum of ?shes in Meghalaya, Northeast India: A checklist. Journal of Parasitogy Diseases, v. 40, p. 312-329, 2016.

Lie, K. J.; Basch, P. F. The Life History of Echinostoma paraensei sp. n. (Trematoda: Echinostomatidae). Journal of Parasitology, v. 5, p. 1192-1199, 1967.

Maji, A. K.; Bera, D. K.; Manna, B.; Nandy, A.; Addy, M.; Bandyopadhyay, A. K. First record of human infection with Echinostoma malayanum in India. Transactions of the Royal Society of Tropical Medicine & Hygiene, v. 87, p. 673, 1993. https://doi.org/10.1016/0035-9203(93)90285-x

Martin, T. R.; Conn, D. B. The pathogenicity, localization, and cyst structure of echinostomatid metacercariae (trematoda) infecting the kidneys of the frogs Rana clamitans and Rana pipiens. Journal of Parasitology, v. 76, p. 414-419, 1990.

Nasir, P. Studies on the life history of Echinostoma nudicaudatum n. sp. (Echinostomatidae: Trematoda). Journal of Parasitology, v. 46, p. 833-847, 1960.

Poulin, R.; Mouillot, D. Host specificity and the probability of discovering species of helminth parasites. Parasitology, v. 130, p. 709-715, 2005.

Sah, R.; Khadka, S.; Hamal, R.; Poudyal, S. Human echinostomiasis: A case report. BMC Research Notes, 11:17, 2018. https://doi.org/10.1186/s13104-018-3133-Z

Seppänen, E.; Kuukka, H.; Voutilainen, A.; Huuskonen, H.; Peuhkuri, N. Metabolic depression and spleen and liver enlargement in juvenile Arctic charr Salvelinus alpinus exposed to chronic parasite infection. Journal of Fish Biology, v. 74, p. 553-561, 2009. https://doi.org/10.1111/j.1095-8649.2008.02144.x

Sripa, B.; Kaewkes, S.; Intapan, P. M.; Maleewong, W.; Brindley, P. J. Food-borne trematodiases in Southeast Asia: Epidemiology, pathology, clinical manifestation and control. Advances in Parasitology, v. 72, p. 305-350, 2010. https://doi.org/10.1016/S0065-308X(10)72011-X

Tandon, V.; Shylla, J. A.; Ghatani, S.; Athokpam, V. D.; Sahu, R. Neglected Tropical Diseases: Trematodiases - The Indian Scenario. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, v. 85, no. 4, p. 901-907, 2014. https://doi.org/10.1007/s40011-014-0465-x

Toledo, R.; Esteban, J. G. An update on human echinostomiasis. Transactions of the Royal Society of Tropical Medicine & Hygiene, v. 110, p. 37-45, 2016. https://doi.org/10.1093/trstmh/trv099

Toledo, R.; Esteban, J. G.; Fried, B. Recent advances in the biology of echinostomes. Advances in Parasitology, v. 69, p. 147-204, 2009. https://doi.org/10.1016/S0065-308X(09)69003-5

Yamaguti, S. Systema helminthum. In: Yamaguti, S. (Ed.). The digenetic trematodes of vertebrates. New York: Interscience, 1958. v. 1.



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