Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 6, no. 13 (2019) Yusuf


Vol. 6, No. 13, p. 381-389 - Aug. 31, 2019


Antisnake venom activity and isolation of quercetin from the leaf of Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae)

A. J. Yusuf , M. I. Abdullahi , A. M. Musa , A. K. Haruna , V. Mzozoyana and H. Abubakar

Snake envenomation is a major cause of death and disability in many developing countries. Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae) have been reportedly used in traditional medicine to treat snake envenomation. Bioassay-guided isolation of antivenom principles was carried out on the leaf of N. macrophylla against Naja nigricollis venom. The methanol extract of N. macrophylla leaf and its ethylacetate and n-butanol fraction significantly (P < 0.05) protected mice against venom-induced lethality with 100% survival rate and there was remarkable inhibition of the poisonous effects of PLA2 enzyme by the extracts and the fractions. Encouraged by this result, the ethylacetate soluble fraction was subjected to purification using vacuum liquid chromatography and gel filtration which led to the isolation of quercetin as the bioactive principle. The identity of the compound was determined on the basis of chemical tests, and by comparison of its 1H-NMR data with literature, this is the first report of isolation of this compound from the leaf of the plant. However, the results of the study suggests that the leaf of N. macrophylla possess significant antisnake venom activity which provide the scientific basis for its use in traditional treatment of snakebites.

Neocarya macrophylla; Leaf; Antivenom; Quercetin; NMR.


Full text

Abubakar, M. S.; Sule, M. I.; Pateh, U. U.; Abdulrahman, E. M.; Haruna, A. K.; Jahun, B. M. In vitro snake venom detoxifying action of the leaf extract of Guiera senegalensis. Journal of Ethnopharmacology, v. 69, no. 3, p. 253-257, 2000.

Aleku, G. A.; Adoga, M. P.; Agwale, S. HIV point-of-care diagnostics: Meeting the special needs of sub-Saharan Africa. The Journal of Infection in Developing Countries, v. 8, no. 10, p. 1231-1243, 2014.

Alexander, V. A. D.; Radhakrishnan, A.; Subramann, P. Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacognosy Review, v. 10, no. 20, p. 84-89, 2016.

Alirol, E.; Sharma S. K.; Bawaskar S. H.; Kuch U.; Chappuis F. Snake bite in South Asia: A review. PLoS Neglected Tropical Diseases, v. 4, no. 1, e603, 2010.

Bhat, M. K.; Kasturi, S.; Gowda, T. V. Structure-function relationships among neurotoxic phospholipases: NN-XIII-PLA2 from Indian cobra (Naja naja naja) and VRV PL-V from Russell's viper (Vipera russelli) venoms. Toxicon, v. 29, no. 1, p. 97-105, 1991.

Carnevale Neto, F.; Pilon, A. C.; Bolzani, B. V. S.; Castro-Gamboa, I. Chrysobalanaceae: Secondary metabolites, ethnopharmacology and pharmacological potential. Phytochemistry Reviews, v. 12, no. 1, p. 121-146, 2013.

Chippaux, J. P. Estimate of the burden of snakebites in Sub-Saharan Africa: A meta-analytic approach. Toxicon, v. 57, no. 4, p. 586-599, 2011.

Chippaux, J. P.; Goyffon, M. Antivenom serotherapy: Its applications, its limitations, its future. Bulletin de la Société de Pathologie Exotique, v. 84, no. 3, p. 286-297, 1991.

Chippaux, J. P.; Rakotonirina, V. S.; Rakotonirina, A.; Dzikouk, G. Drug or plant substances which antagonize venoms or potentiate antivenins. Bulletin de la Société de Pathologie Exotique, v. 90, no. 4, p. 282-285, 1997.

Daltry, J. C.; Wuster, W.; Thorpe, R. S. Diet and snake evolution. Nature, v. 379, p. 537-540, 1996.

Dechambenoit G. Access to health care in Sub-Saharan Africa. Surgical Neurology International, 7:108, 2016.

Finney, D. J. Probit analysis. 3. ed. Cambridge: Cambridge University Press, 1977.

Haslam, E.; Lilley, T. H.; Cai, Y.; Martin, R.; Magnolato, D. Traditional herbal medicines-the role of polyphenols. Planta Medica, v. 55, no. 1, p. 1-8, 1989.

Isa, H. I.; Ambali, S. F.; Suleiman, M. M.; Abubakar, M. S.; Kawu, M. U.; Shittu, M.; Yusuf, P. O.; Habibu, B. In vitro neutralisation of Naja nigricollis venom by stem-bark extracts of Commiphora africana A. Rich. (Burseraceae). Journal of Environmental Science, Toxicology and Food Technology, v. 9, no. 12, p. 100-105, 2015.

Lindahl, M.; Tagesson, C. Flavonoids as phospholipase A2 inhibitors: Importance of their structure for selective inhibition of group II phospholipase A2. Inflammation, v. 21, no. 3, p. 347-356, 1997.

Lorke, D. A New approach to practical acute toxicity testing. Archives of Toxicology, v. 54, no. 4, p. 275-287, 1983.

Mabry, T. J.; Markham, K. R.; Thomas, Y. B. The systematic identification of flavonoids. Berlin: Springer-Verlag Publication, 1970.

Macfarlane, R. G. Russel's viper's venoms, 1963-1964. British Journal of Haematology, v. 13, p. 437-451, 1967.

Mahanta, M.; Mukherjee, A. K. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja nigricollis venom by Mimosa pudica root extracs. Journal of Ethnopharmacology, v. 75, no. 1, p. 55-60, 2001.

Melo, P. A.; Ownby, C. L. Ability of wedelolactone, heparin, and para-bromophenacyl bromide to antagonize the myotoxic effects of two crotaline venoms and their PLA2 myotoxins. Toxicon, v. 37, no. 1, p. 199-215, 1999.

Nicholson, R. L.; Hammerschmidt, R. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, v. 30, p. 369-389, 1992.

Ode, O. J.; Asuzu, I. U. The anti-snake venom activities of the Crinum jagus (Amaryllidaceae). Toxicon, v. 48, no. 3, p. 331-342, 2006.

Sani, Y. M.; Musa, A. M.; Abdullahi, S. M.; Nasir, T.; Abdullahi, M. I.; Atiku, I. Quercetin and β-sitosterol isolated from the methanol leaves extract of Cissus polyantha Glig and Brandt (Vitaceae). Nigerian Journal of Pharmacology Sciences, v. 14, no. 2, p. 46-50, 2015.

Silva, G. L.; Lee I.; Douglas K. A. Special problems with extraction of plants. In: Cannell, J. P. R. (Eds.). Natural products isolation. New Jersey, USA: Human Publishers, 1998. p. 251-293.

Stocker, K. F. Composition of snake venoms. In: Stocker, K. F. (Ed.). Medical use of snake venom proteins. Boston: CRC Press, 1990.

Tan, N. H.; Tan, C. S. Acidimetric assay of phospholipase A using egg yolk suspension as substrate. Analytical Biochemistry, v. 170, no. 2, p. 282-288, 1988.

Theakston, R. D. G.; Reid, H. A. The development of simple standard assay procedures for characterization of snake venoms. Bulletin WHO, v. 61, p. 946-956, 1983.

Tsai, L. H.; Yang, L. L.; Chang, C. Inactivation of Formosan snake venoms in vivo by aristolochic acid the chemical components of Aristolocia radix. Tai-Wan Ko'Hsueh, v. 34, no. 2, p. 40-44, 1980.

Yusuf, A. J.; Abdullahi, M. I.; Aleku, G. A.; Ibrahim, I. A. A.; Alebiosu, C. O.; Yahaya, M.; Adamu, H. W.; Sanusi, A.; Mailafiya, M. M.; Abubakar, H. Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla. Journal of Medical Plants for Economic Development, v. 2, no. 1, a38, 2018.

Yusuf, A. J.; Abdullahi, M. I.; Haruna, A. K.; Idris, A. Y.; Musa, A. M. Preliminary phytochemical screening, toxicological and antivenin property of the stem bark of Neocarya macrophylla on Naja nigricollis venom. African Journal of Pharmaceutical Research and Development, v. 7, no. 1, p. 6-10, 2015a.

Yusuf, A. J.; Abdullahi, M. I.; Haruna, A. K.; Idris, A. Y.; Musa, A. M. Isolation and characterization of stigmasterol and Bis-(5,7-diacetyl-catechin-4'-α-rhamnopyranoside) from the stem bark of Neocarya macrophylla (Sabine) Prance (Chrysobalanaceae). Nigerian Journal of Basic and Applied Sciences, v. 21, no. 1, 15-22, 2015b.

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