Vol. 6, No. 13, p. 313-329 - Aug. 31, 2019
Nutritional, haematinic and biosafety evaluation of Ipomoea batata (L.) Lam. (Solanales: Convolvulaceae) leaf extract on albino rats
Benjamin O. Gabriel and MacDonald Idu
Abstract
This study evaluates the nutritional, Haematinic and biosafety of Ipomoea batatas (L.) Lam. (Solanales: Convolvulaceae) aqueous leaf extract on albino rats. Proximate and mineral compositions were determined using standard methods. Haematinic activity of the plant was done using graded doses; 125, 250, and 500 mg/kg and 40 mg/kg phenyl hydrazine hydrochloride to induce anaemia. A modified method was used for acute and sub-acute toxicological evaluations. Results from the study showed that aqueous extracts of I. batatas had significant increase in RBC, HCT, Hgb, MCV, MCH and MCHC at 7.19±0.45, 46.13±0.08, 13.43±0.27, 82.40±0.92, 24.15±1.24 and 37.78±0.20, respectively, when compared with the control group. Acute study showed no pathological behaviour with absent mortality. Sub-acute study of the spleen, heart, liver and kidney showed a mild activation of local immune system. The extracts recorded 89.14% scavenging property compared with 94.3% ascobate in antioxidant study. Proximate analysis had 31.56% of moisture content, 16.25% of protein, 7.64% of ash, 0.37% of crude fibre, 0.19% of fat and 43.99% of carbohydrate. Investigation of calcium, magnesium, Iron, potassium, phosphorus, sodium and zinc were 28.03, 339.61, 15.87, 4.61, 35.90, 4.21 and 0.08 mg/kg others were not detected. This may be due to stimulating mechanism of Myelo-Erythroid cell ratio in bone marrow or antioxidant effect. Result thus validates ethno-botanical uses of I. batatas for the treatment of anaemia.
Keywords
Sweetpotato; Anaemia; Bone marrow; Peripheral blood smear; Phenylhydrazine hydrochloride.
DOI
10.21472/bjbs.061301
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References
Adebayo, J. O.; Adesokan, A. A.; Olatunji, L. A.; Buoro, D. O.; Soladoye, A. O. Effect of ethanolic extract of
Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biokemistri,
v. 17, p. 45-70, 2005. https://doi.org/10.4314/biokem.v17i1.32588
Antia, B. S.; Akpan, E. J.; Okon, P. A.; Umoren, I. U. Nutritive and anti-nutritive evaluation of sweet potatoes
(Ipomoea batatas) leaves. Pakistan Journal of Nutrition, v. 5, no. 2, p. 166-168, 2006. https://doi.org/10.3923/pjn.2006.166.168
AOAC - Association of Official Analytical Chemistry International. Official method of analysis. 18 ed.
Maryland: AOAC, 2005.
Bratosin, D.; Mazurier, J.; Tissier, J. P.; Estaquier, J.; Huart, J. J.; Ameisen, J. C.; Aminoff, D.; Montreuil,
J. Cellular and molecular mechanisms of senescent erythrocyte phagocytosis by macrophages: A review. Biochimie,
v. 80, no. 2, p. 173-195, 1998. https://doi.org/10.1016/S0300-9084(98)80024-2
Claro, L. M.; Leonart, M. S.; Comar, S. R.; Nascimento, A. J. Effect of vitamin C and E on oxidative processes in
human erythrocytes. Cell Biochemistry & Function, v. 24, no 6, p. 531-535, 2006. https://doi.org/10.1002/cbf.1255
Cole, E. H. Veterinary clinical pathology. Philadelphia: W. B. Sander, 2006.
Dini, I.; Tenore, G. C.; Dini, A. Saponins in Ipomoea batatas tuber: Isolation, characterization, quantification
and antioxidant properties. Food Chemistry, v. 113, p. 411-419, 2009. http://doi.org/10.1016/j.foodchem.2008.07.053
Drury, R. A. B.; Wallinton, E. A. Carletons histological technique. 16. ed. London: Oxford University Press, 2003.
Falodun, A.; Irabor, E. I. Phytochemical, proximate, antioxidant and free radicals scavenging evaluation of Calliandria
surinamensis. Acta Poloniae Pharmaceutica - Drug Research, v. 65, no. 5, p. 571-575, 2008.
Gasting, D.; Nkeugouapi, C. F. N.; Nkah, B. F. N.; Kuiate, J. R.; Tcheuanguep, F. M. Antibacterial activity, bioavailability
and acute toxicity evaluation of the leaf extract of Alchornea cordifolia (Euphorbiaceae). International Journal of
Pharmacology, v. 6, p. 173-182, 2010. https://doi.org/10.3923/ijp.2010.173.182
Idu, M.; Erhabor, J. O.; Timothy, O.; Etatuvie, S. O. Phytochemical and acute toxicity studies of the aqueous extract and
methanol extracts of Emilia coccinea (Sims.) G. Dm. Journal of Plant Development Sciences, v. 2, no. 3/4,
p. 89-94, 2010.
Kaliora, A. C.; Dedoussis, G. V. Z.; Schmidt, H. Dietary antioxidants in preventing atherogenesis. Artherosclerosis,
v. 187, no. 1, p. 1-17, 2006. https://doi.org/10.1016/j.atherosclerosis.2005.11.001<
Komolafe, O. I.; Awoniyi, A. O. The prevalence of microbial isolates associated with infertility in men attending clinic
in OAUTHC, Ile-Ife. International Journal of Microbiology Research and Reviews, v. 1, no. 5, p. 88-91, 2013.
Konczak-Islam, I.; Yoshimoto, Y.; Hou, D.; Terahara, N.; Yamakawa, O. Potential chemopreventive properties of anthocyanin-rich
aqueous extracts from in vitro produced tissue of sweetpotato (Ipomoea batatas L.). Journal of Agricultural
Food Chemistry, v. 51, p. 5916-5922, 2003. https://doi.org/10.1021/jf030066o
Leong, L. P.; Shui, G. An investigation of antitoxic capacity of fruit in Singapore markets. Food Chemisty, v. 76, no. 1,
p. 69-75, 2002. https://doi.org/10.1016/S0308-8146(01)00251-5
Lessin, L. S.; Klug, P. P.; Jensen, W. N. Clinical implications of red cell shape. In: Stollerman, G. H. Advances in Internal
Medicine. Chicago: Year Book Medical Publishers, 2006. v. 21. p. 451-499.
Lippi, G.; Schena, F.; Salvagno, G. L.; Aloe, R.; Banfi, G.; Guidi, G. C. Foot-strike haemolysis after a 60-km ultramarathon.
Blood Transfusion, v. 10, no. 3, p. 377-383, 2012. https://doi.org/10.2450/2012.0167-11
Moswa, J. L.; Kapanda, N.; Mungende, D. M.; Okitolonda, W.; Mayangi, M.; Mihigo, S. Plant as an important source of iron for
the treatment of anaemia: Case of Justicia secunda. Proceedering of the XI NARECA, Madagascar, Antananarivo,
p. 132-135, 2005.
Noda, N.; Horiuchi, V. The resin glycosides from the sweet potato (Ipomoea batatas L. LAM.). Chemical Pharmacology
Bulletin, v. 56, no. 11, p. 1607-1610, 2008. https://doi.org/10.1248/cpb.56.1607
Odesanmi, O. S.; Ojokuku, S. A.; Apena, A.; Bikomo, O. E.; Lawal, R. A. Nutritional prospect of an aphrodisiac Microdesmis
keayana. Journal of Medicinal Plants Research, v. 6, no. 7, p. 1187-1190, 2012.
Ozaki, S.; Oki, N.; Suzuki, S. Structural characterization and hypoglycemic effects of arabinogalactan-protein from the tuberous
cortex of the white-skinned sweet potato (Ipomoea batatas L.). Journal on Agricultural Food Chemistry, v. 58,
no. 22, p. 332-361, 2010. https://doi.org/10.1021/jf101283f
Rabah, I. O.; Hou, D. X.; Komine, S. I.; Fujii, M. Potential chemopreventive properties of extract from baked sweet potato
(Ipomoea batatas Lam. Cv. Koganesengan). Journal of Agricultural Food Chemistry, v. 52, no. 23, p. 5916-5922,
2004. https://doi.org/10.1021/jf049368w
Sen, G.; Mandal, S.; Saha, R. S.; Mukhopadhyay, S.; Biswas, T. Therapeutic use of quercetin in the control of infection and
anaemia association with visceral leishmaniasis. Free Radical Biology and Medicine, v. 38, no. 9, p. 1257-1264, 2005.
https://doi.org/10.1016/j.freeradbiomed.2005.01.014
Smith, A.; Edufunke, L. O.; Adeyemi, R. O.; Adeyemi, O. A.; Lawson, A. A. Toxicity test of water homogenate of Cissus
populnea stem in mice and its effect on weight of some organs, serum gonadotrophins, lipids and uric acids in male
albino rats. Nigerian Quarterly Journal of Hospital Medicine, v. 10, p. 224 227, 2002. https://doi.org/10.4314/nqjhm.v10i3.12475
Suda, I.; Oki, T.; Masuda, M.; Kobayashi, M.; Nishiba, Y.; Furuta, S. Physiological functionality of purple-freshed sweet
potatoes containing anthocyanins and their utilization in food. Japan Agricultural Resource Quarter, v. 37, no. 3,
p. 167-173, 2003. https://doi.org/10.6090/jarq.37.167
Telford, R. D.; Sly, G. J.; Hahn, A. G.; Cunningham, R. B.; Bryant, C.; Smith, J. A. Footstrike is the major cause of hemolysis
during running. Journal on Applied Physiology, v. 94, no. 1, p. 38-42, 2003. https://doi.org/10.1152/japplphysiol.00631.2001
Yakubu, M. T.; Afolayan, A. J. Effect of aqueous extract of Bulbine natalensis Baker stem on haematological and serum
lipid profile of male wistar rats. Indian Journal of Experimental Biology, v. 47, no. 4, p. 283-288, 2009.
Yakubu, M. T.; Akanji, M. A.; Oladiji, A. T. Hematological evaluation in male albino rats following chronic administration of
aqueous extract of Fadogia argrestis stem. Pharmacognosy Magazine, v. 3, p. 34-47, 2007.
Yamoto, O.; Maede, Y. Susceptibility to onion-induced hemolysis in dogs with hereditary high erythrocyte reduced glutathione
and potassium concentrations. American Journal of Veterinary Research, v. 53, no. 1, p. 134-142, 2002.
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