Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 6, no. 12 (2019) Parveen


Vol. 6, No. 12, p. 133-139 - Apr. 30, 2019


Effect of culture filtrates of pathogenic and antagonistic fungi on seed germination of some economically important vegetables

Shazia Parveen , Abdul Hamid Wani and Mohd Yaqub Bhat

The subject of present study was to check whether the pathogenic fungi that were associated with different rot diseases of fruits and vegetables and the antagonistic fungal species produce extracellular growth regulating substances. For this present study healthy seeds of four economically important crop plants, viz. Solanum lycopersicum, Brassica rapa, Raphanus sativus and Trigonella melongena were selected. The results showed that all the pathogenic fungi except Fusarium solani decrease the germination percentage of the all seeds. Solanum lycopersicum seed germination was completely inhibited by the culture filtrate of Trichothecium roseum and Alternaria alternata. Likewise, the culture filtrate of Penicillium expansum caused complete inhibition of the germination of Brassica rapa seeds. The culture filtrate of Fusarium solani was found to increase the germination percentage of all the seeds tested during the present study. Amongst the three Trichoderma spp., T. asperellum and T. harzianum culture filtrate effectively increases the seed germination percentage of all the seeds tested while the culture filtrate of T. viride have negative effect on the germination percentage of Solanum lycopersicum, Brassica rapa, and Raphanus sativus seeds. This stimulatory or inhibitory effect of the culture filtrates can be attributed to the presence of certain metabolites/substances that the test fungi have released in the medium. To identify the substances present and the nature of these substances further studies will be carried out.

Antagonistic fungi; Culture filtrate; Pathogenic fungi; Seed germination.


Full text

Alwakeel, S. S. Molecular identification of isolated fungi from stored apples in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences, v. 20, p. 311-317, 2013.

Bhale, U. N. Survey of market storage diseases of some important fruits of Osmannabad district, India. Science Research Reporter, v. 1, no. 2, p. 88-91, 2011.

Caster, L.; Frederikson, R. A. Fusarium head blight, occurrence of effects on sorghum yield and grain characteristics in Texas. Journal of Plant Disease, v. 64, p. 1017-1019, 1980.

Celar, F.; Valic, N. Effects of Trichoderma spp. and Gliocladium roseum culture filtrate on seed germination of vegetable and maize. Journal of Plant Diseases and Protection, v. 112, no. 4, p. 343-350, 2005.

Chang, Y. C.; Baker, R.; Kleifeld, O.; Chet, I. Increased growth of plants in the presence of the biological control agent Trichoderma harzianum. Plant Disease, v. 70, p. 145-148, 1986.

Cole, J. S.; Zvenyika, Z. Integrated control of Rhizoctonia solani and Fusarium solani in tobacco transplants with Trichoderma harzianum and Triadimenol. Plant Pathology, v. 37, p. 271-277, 1988.

Garuba, T.; Abdulrahaman, A. A.; Olahan, G. S.; Abdulkareem, K. A.; Amadi, J. E. Effects of fungal filtrates on seed germination and leaf anatomy of maize seedlings (Zea mays L., Poaceae). Journal of Applied Sciences and Environmental Management, v. 18, no. 4, p. 662-667, 2014.

Gupta, O.; Sharma, N. D. Effect of fungal metabolites on seed germination and root length of black gram (Phaseolus mungo L.). Legume Research, v. 18, p. 64-66, 1995.

Haikal, N. Z. Effect of filtrates of pathogenic fungi of soyabean on seed germination and seedling parameters. Journal of Applied Sciences Research, v. 4, no. 1, p. 48-52, 2008.

Inber, J.; Mendez, I.; Chet, I. Hyphal interaction between Trichoderma harzianum and Sclerotinia sclerotium and its role in biological control. Soil Biology and Biochemistry, v. 28, p. 757-763, 1996.

Jalander, V. Gachande, B. D. Effect of fungal metabolites of some rhizospheric soil fungi on seed germination and seedling growth of some pulses and cereals. Science Research Reporter, v. 2, no. 3, p. 265-267, 2012.

Janisiewicz, W. J.; Korsten, L. Biological Control of post-harvest diseases of fruits. Annual Review of Phytopathology, v. 40, p. 411-441, 2002.

Karman, M.; Malavuly, M. Heavy metals and macro elements in some macro fungi of national park Frushkagora (Serbia). Proceeding of the 8th ISIRR-International Symposium on Interdisciplinary Regional Research, 2005. p. 1-10.

Kohl, J.; Schlosser, E. Effect of Trichoderma spp. on seedlings of sugar beet during the biological control of pathogens. Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniver-siteit Gent, v. 54/2b, p. 707-714, 1987.

Kunwar, I. K.; Mehrotra, B. S. Effect of culture filtrates of storage fungi on germination and sprouting of wheat grains. Proceedings of Indian National Science Academy, v. B54, no. 6, p. 399-400, 1988.

Kunwar, I. K.; Halfon-Meiri, A.; Manandhar, J. B.; Sinclair, J. B. Possible phytotoxic metabolites in the culture filtrates of Diaporthe phaseolarum var. sojae. Mycopathologia, v. 99, p. 71-79, 1987.

Lindsey, D. L.; Baker, R. Effect of certain fungi on dwarf tomatoes grown under gnotobiotic conditions. Phytopathology, v. 57, p. 1262-1263, 1967.

Madhosing, C. Relative wilt-inducing capacity of the culture filtrates of isolates of Fusarium oxysporum f. sp. radicis-lycopersici, the tomato crown and root rot pathogen. Journal of Phytopathology, v. 4, p. 193-198, 1995.

Negi, H. C. S.; Jain, S. K.; Singh, R. V. Seed dormancy in radish. Seed Research, v. 11, p. 60-62, 1983.

Parveen, S.; Wani, A. H. In vitro efficacy of some fungicides and plant extracts on Mucor piriformis Fisher causing postharvest rot of peach in Kashmir Valley. Trends in Biosciences, v. 8, no. 3, p. 620-627, 2015.

Parveen, S.; Wani, A. H.; Bhat, M. Y.; Pala, S. A.; Ganie, A. A. Biology and management of Aspergillus niger Van Tiegh. causing black mold rot of pear (Pyrus communis L.) in Kashmir Valley, India. International Journal of Advanced Research, v. 2, no. 6, p. 24-34, 2014.

Parveen, S.; Wani, A. H.; Ganie, A. A.; Pala, S. A.; Mir, R. A. Antifungal activity if some plant extracts on some pathogenic fungi. Archives of Phytopathology and Plant Protection, v. 47, no. 3, p. 279-284, 2013.

Roy, R. Y.; Dwivedi, R. S.; Gupta, S. Effect of rhizospheric mycoflora on the growth of Trigonella foenum-graceum. Proceedings of National Academy of Science of India, v. 42, p. 105-110, 1972.

Umecharuba, G. I.; Nwachukwa, E. O. The effect of filtrate of seed borne fungi of African yam bean on seed germination and seedling development. Global Journal of Pure and Applied Sciences, v. 3, p. 165-175, 1997.