Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 6, no. 12 (2019) Raghavan


Vol. 6, No. 12, p. 87-101 - Apr. 30, 2019


Effect of ecdysteroids on oogenesis in the freshwater crab Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae)

Sudha Devi Arath Raghavan and Aswani Ayanath

This study evaluated the reproductive performance of female Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae) administered with 20-OH ecdysone during different phases of the oogenic cycle. The effect of administration was evaluated by comparing the mean gonadosomatic index, oocyte diameter, oocyte proportion values and histological features of the control and concurrent control ovaries with those of the experimentals. The results clearly indicated that 20-OH ecdysone can stimulate ovarian growth and maturation in all phases of the oogenic cycle, though it caused statistically significant effects only during the early and middle vitellogenic phases, evidenced from the accelerated gonadosomatic index, oocyte diameter and oocyte proportion values, occurrence of vitellogenic oocytes in avitellogenic and previtellogenic ovaries, increased degree of yolk deposition and proliferation of gonia in the experimental ovaries compared to the controls. The outcome of this study is quite promising in the aquaculture practice of this locally abundant edible freshwater crab which forms a cheap protein substitute for the malnourished tribes/poor people of Wayanad.

20-OH ecdysone; Freshwater crab; Oogenesis; ovarian growth; Travancoriana schirnerae.


Full text

Adiyodi, R. G.; Subramoniam, T. Arthropoda: Crustacea. In: Adiyodi, K. G.; Adiyodi, R. G. (Eds). Reproductive Biology of Invertebrates. Vol. I Oogenesis, oviposition and oosorption. New York: John Wiley and Sons, 1983. p. 443-495.

Arvy, L.; Echalier, G.; Gabe, M. Modifications de la gonade de Carcinus maenas apres ablation bilaterale de l'organe Y. Comptes Rendus de l'Académie des Sciences, Serie D, v. 239, p. 1853-1855, 1954.

Blanchet-Tournier, M. F. Quelques aspects des interactions hormonales entre la mue et la vitellogenèse chez le Crustacé Amphipode Orchestia gammarellus (Pallas). Reproduction Nutrition Development, v. 22, no. 2, p. 325-344, 1982.

Brandau, H. Histochemical localization of enzyme activities in normal and gonadotrophin stimulated mouse ovaries. In: Butt, W. R.; Crooke, A. C.; Ryle, M. (Eds.). Gonadotrophins and Ovarian Development. Livingstone, Edinburgh, London: Eshkol, A., Lunenfeld, B., and Pet, 1970. p. 307-311.

Brown, M.; Sieglaff, D.; Rees, H. Gonadal ecdysteroidogenesis in Arthropoda: Occurrence and regulation. Annual Review of Entomology, v. 54, p. 105-125, 2009.

Chaix, J. C.; De Reggi, M. Ecdysteroid levels during ovarian development and embryogenesis in the spider crab Acanthonyx lunulatus. General and Comparative Endocrinology, v. 47, no. 1, p. 7-14, 1982.

Chan, S. M. Possible roles of 20-hydroxyecdysone in the control of ovary maturation in the white shrimp Penaeus vannamei (Crustacea: Decapoda). Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, v. 112, no. 1, p. 51-59, 1995.

Chang, E. S. Hormonal control of molting in decapod Crustacea. American Zoologist, v. 25, no. 1, p. 179-185, 1985.

Couch, E. F.; Hagino, N.; Lee. J. W. Changes in estradiol and progesterone immunoreactivity in tissues of the lobster, Homarus americanus, with developing and immature ovaries. Biochemistry and Physiology Part A: Molecular & Integrative Physiology, v. 87, no. 3, p. 765-770, 1987.

Crompton, D. W. Studies on the haemocytic reaction of Gammarus spp., and its relationship to Polymorphus minutus (Acanthocephala). Parasitology, v. 57, n. 2, p. 389-401, 1967.

Démeusy, N. Rôle de la gland de mue dans l'évolution ovarianne due crabe Carcinus maenas Linn. Cahiers de Biologie Marine, v. 3, p. 37-56, 1962.

Gong, J.; Ye, H.; Xie, Y.; Yang, Y.; Huang, H.; Li, S.; Zeng, C. Ecdysone receptor in the mud crab Scylla paramamosain: A possible role in promoting ovarian development. Journal of Endocrinology, v. 224, no. 3, p. 273-287, 2015.

Gunamalai, V.; Kirubagaran, R.; Subramoniam, T. Sequestration of ecdysteroid hormone into the ovary of the mole crab, Emerita asiatica (Milne Edwards). Current Science, v. 85, no. 4, p. 493-496, 2003.

Kale, R. S.; Shejule, K. B.; Kharat, P. S.; Sonawane, A. K. Induce ovarian maturation by 17α-hydroxyprogesterone hormone in freshwater crab, Barytelphusa cunicularis. Journal of Aquatic Biology, v. 23, no. 2, p. 109-112, 2008.

Kale, R. S. Effect of β-estradiol hormone and eye stalk ablation on ovarian maturation in fresh water crab, Barytelphusa cunicularis. Bioscience Discovery, v. 8, no. 2, p. 140-145, 2017.

Kulkarni, G. K.; Nagabhushanam, R.; Joshi, P. K. Effect of progesterone on ovarian maturation in a marine penaeid prawn Parapenaeopsis hardwickii (Miers, 1878). Indian Journal of Experimental Biology, v. 17, p. 986-987, 1979.

Lachaise, F.; Hoffmann, J. A. Ecdysone et developement ovarien chez un Decapode, Carcinus maenas. Comptes Rendus de l'Académie des Sciences, Serie D, v. 285, p. 701-704, 1981.

Lachaise, F.; Goudeau, M.; Hetru, C.; Kappler, C.; Hoffmann, J. A. Ecdysteroids and ovarian development in the shore crab, Carcinus maenas. Hoppe-Seyler's Zeitschrift für Physiologische Chemie, v. 362, no. 1, p. 521-530, 1981.

Lanot, R.; Cledon P. Ecdysteroids and meiotic reinitiation in Palaemon serratus (Crustacea Decapoda Natantia) and in Locusta migratoria (Insecta Orthoptera): A comparative study. Invertebrate Reproduction & Development, v. 16, no. 1/3, p. 169-175, 1989.

Laufer, H.; Ahl, J. S.; Sagi, A. The role of juvenile hormones in crustacean reproduction. American Zoologist, v. 33, no. 3, p. 365-374, 1993.

Laufer, H.; Borst, D. W.; Foley, T. A.; Landau, M. Ecdysteroid titres in vitellogenic Libinia emarginata. Journal of Insect Physiology, v. 34, no. 7, p. 615-617, 1988.

Medesani, D. M.; Ferre, L. E.; Canosa, I. S.; Silveyra, G. R.; Rodriguez, E. M. Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata. Invertebrate Reproduction & Development, v. 59, no. 2, p. 1-7, 2015.

Merlin, J.; Mohanlal, D. L.; Balasubramanian, C. P.; Sherly, T.; Subramoniam, T.; Syamadayal, J.; Ravichandran, P.; Ponniah, A. G.; Gopal, C.; Vijayan, K. K. Induction of vitellogenesis and reproductive maturation in tiger shrimp, Penaeus monodon by 17β-estradiol and 17α-hydroxyprogesterone: In vivo and in vitro studies. Invertebrate Reproduction & Development, v. 59, no. 3, p. 166-175, 2015.

Mu, C.; Song, W.; Li, R.; Chen, Y.; Hao, G.; Wang, C. Identification of differentially expressed proteins relating to ovary development in Portunus trituberculatus. Aquaculture, v. 426, p. 148-153, 2014.

Muhd-Farouk, H.; Abol-Munafi, A.; Jasmani, S.; Ikhwanuddin, M. Effect of steroid hormones 17α-hydroxyprogesterone and 17α-hydroxypregnenolone on ovary external morphology of orange mud crab, Scylla olivacea. Asian Journal of Cell Biology, v. 9, p. 23-28, 2014.

Muhd-Farouk, H.; Jasmani, S.; Ikhwanuddin, M. Effect of vertebrate steroid hormones on the ovarian maturation stages of orange mud crab, Scylla olivacea (Herbst, 1796). Aquaculture, v. 451, p. 78-86, 2016.

Nagabhushanam, R.; Sambasivarao, S.; Sarojini, R.; Jayalakshmi, K. Annual reproductive cycle of female Metapenaeus affinis. Proceeding of the National Symposium on Physiology of Crustaceans, p. 39-41, 1987.

Nagabhushanam, R.; Joshi, P. K.; Kulkarni, G. K. Induced spawning in prawn Parapenaeopsis stylifera (H. Milne Edwards) using a steroid hormone 17 hydroxy-progesterone. Indian Journal of Marine Sciences, v. 9, p. 227, 1980.

Okumura, T.; Sakiyama, K. Hemolymph levels of vertebrate-type steroid hormones in female kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda: Penaeidae) during natural reproductive cycle and induced ovarian development by eyestalk ablation. Fisheries Science, v. 70, p. 372-380, 2004.

Okumura, T.; Aida, K. Fluctuations in hemolymph ecdysteroid levels during the reproductive and non-reproductive molt cycles in the giant freshwater prawn Macrobrachium rosenbergii. Fisheries Science, v. 66, no. 5, p. 876-883, 2000.

Okumura, T.; Han, C. H.; Suzuki, Y.; Aida, K.; Hanyu, I. Changes in hemolymph vitellogenin and ecdysteroid levels during the reproductive and non-reproductive molt cycles in the freshwater prawn Macrobrachium rosenbergii. Zoological Science, v. 9, p. 37-45, 1992.

Reddy, B. S.; Sridevi, V.; Neelima, H.; Reddy, P. R.; Reddy, P. S. Regulation of vitellogenesis by selected endocrine modulators in crab Oziothelphusa senex senex, with special reference to methyl farnesoate. Aquaculture Reports, v. 3, p. 24-30, 2016.

Reddy, P. R.; Kiranmayi, P.; Kumari, K.; Reddy, P. S. 17α-hydroxyprogesterone induced ovarian growth and vitellogenesis in the freshwater rice field crab Oziotelphusa senex senex. Aquaculture, v. 254, no. 1/4, p. 768-775, 2006.

Rodríguez, E. M.; Medesani, D. A.; Greco, L. S. L.; Fingerman, M. Effects of some steroids and other compounds on ovarian growth of the red swamp crayfish, Procambarus clarkii, during early vitellogenesis. Journal of Experimental Zoology, v. 292, no. 1, p. 82-87, 2002.

Sarojini, R.; Jayalakshmi, K.; Sambashivarao, S. Effect of external steroids on ovarian development in the freshwater prawn Macrobrachium lamerrii. Journal of Advanced Zoology, v. 7, no. 1, p. 50-53, 1986.

Sarojini, R.; Mirajkar, M. S.; Nagabhushanam, R. Effect of steroids on the oogenesis and spermatogenesis of the freshwater prawn, Macrobrachium kistnensis. Comparative Physiology and Ecology, v. 10, no. 1, p. 7-11, 1985.

Sarojini, R.; Sambasivarao, S.; Jayalakshmi, K. Effect of steroids (Estradiol and estrone) on the ovaries of the marine crab, Scylla serrata. Comparative Physiology and Ecology, v. 15, no. 1, p. 21-26, 1990.

Steel, C. G. H.; Vafopoulou, X. Ecdysteroid titres in haemolymph and other tissues during moulting and reproduction in the terrestrial isopod, Oniscus asellus (L.). Invertebrate Reproduction & Development, v. 34, no. 2/3, p. 187-194, 1998.

Subramoniam, T. Crustacean ecdysteroids in reproduction and embryogenesis. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, v. 125, no. 2, p. 135-156, 2000.

Sujathamma, A.; Dayakar, Y. Effect of estradiol and 17α-hydroxyprogesterone on ovarian development of freshwater paddy field crab, Oziotelphusa senex senex (Fabricius). European Journal of Experimental Biology, v. 5, n. 9, p. 27-30, 2015.

Swetha, C. H.; Girish, B. P.; Reddy, P. S. Elucidation of the role of estradiol and progesterone in regulating reproduction in the edible crab, Oziothelphusa senex senex. RSC Advances, v. 6, no. 30, p. 24959-24967, 2016.

Tsukimura, B.; Kamemoto, F. L. Organ culture assay of the effects of putative reproductive hormones on immature penaeid ovaries. The World Aquaculture Society Abstract, v. 288, 1988.

Van Herp, F.; Payen, G. G. Crustacean neuroendocrinology: Perspectives for the control of reproduction in aquacultural systems. Bulletin of the Institute of Zoology, Academia Sinica, v. 16, p. 513-539, 1991.

Wongsawang, P.; Phongdara, A.; Chanumpai, A.; Chotigeat, W. Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn. Songklanakarin Journal of Science and Technology, v. 27, p. 789-798, 2005.

Yano, I. Effect of 17-hydroxy-progesterone on vitellogenin secretion in kuruma prawn, Penaeus japonicus. Aquaculture, v. 61, p. 49-57, 1987.

Young, N. J.; Webster, S. G.; Rees, H. H. Ecdysteroid profiles and vitellogenesis in Penaeus monodon (Crustacea: Decapoda). Invertebrate Reproduction & Development, v. 24, no 2, p. 107-117. 1993.

Yudin, A. I.; Diener, R. A.; Clark, Jr. W.; Chang, E. S. Mandibular gland of the blue crab, Callinectes sapidus. The Biological Bulletin, v. 159, p. 760-772, 1980.

Zapata, V.; Lopez Greco, L. S.; Medesani, D.; Rodriguez, E. M. Ovarian growth in the crab, Chasmagnathus granulata induced by hormones and neuroregulators throughout the year: In vivo and in vitro studies. Aquaculture, v. 224, no. 1/4, p. 339. 2003.