Vol. 5, No. 9, p. 143-157 - Apr. 30, 2018
Tissue analysis of Brazilian gracile opossum digestory tube (Gracilinanus microtarsus Wagner, 1842) (Marsupialia: Didelphidae)
Luís Miguel Lobo , Amilton Cesar dos Santos , Ricardo Alexandre Rosa , Gerlane de Medeiros Costa , Ana Flávia Carvalho and Celina Almeida Furlanetto Mançanares
Abstract
The alimentary habit reflects in the morphology of digestive organs. In this sense, microscopic studies are useful to analyze the tissue composition of each organ and allow greater comparisons with other marsupials described in the literature. For this reason, the aim of this study was realize a microscopic description of the digestive tube in Gracilinanus microtarsus Wagner, 1842 (Marsupialia: Didelphidae). Six adults specimens were used (3 males and 3 females). Samples from organs of digestive tube were fixed in 10% folmaldehyde solution. The samples were dehydrated in increasing concentrations of ethanol (70%-100%), diaphanized in xylol, paraffin-embedded, cut into a microtome and stained by H. E., Picrossirius, Schiff periodic acid, Toluidine Blue and Masson's Trichrome. The material was analyzed and photographed through a Nikon Eclipse E-400 Photomicroscope. This study demonstrated, for the first time, the histological constitution of the digestive tube of G. microtarsus. It has been demonstrated that the digestive tube is formed by the esophagus, stomach, small intestine (duodenum, jejunum and ileum) and large intestine (cecum, colon and rectum). These organs were constituted by five distinct layers, a serosa coating externally the organ, a muscular, a submucosa, a muscular of the mucosa, and a mucosa with epithelium that varied between squamous keratinized in the esophagus to prismatic-type in the other organs. This study demonstrated that the digestive tube of animals studied is similar to that described in other marsupials Didelphidae described in the literature with the same food habit. Future immunohistochemical studies may demonstrate the enzymatic complex involved in digestion and the cellular and molecular mechanisms involved in the uptake of nutrients by the digestive tube in this species.
Keywords
Didelphidae; Digestive tract; Digestory apparatus; Marsupialia; Omnivorous.
DOI
10.21472/bjbs.050915
Full text
PDF
References
Aleixo, V. M.; Pressinoti, L. N.; Campos, D. V. S.; Menezes-Aleixo, R. C.; Ferraz, R. H. S. Histologia,
histoquímica e histometria do intestino de jacaré-do-Pantanal criado em cativeiro.
Pesquisa Veterinária Brasileira, v. 31, No. 12, p. 1120-1128, 2011. https://doi.org/10.1590/S0100-736X2011001200014
Banks,W. J. Histologia veterinária aplicada. 2. ed. São Paulo: Manole, 1992.
Cáceres, N. C. Comparative lengths of digestive tracts of seven didelphid marsupials
(Mammalia) in relation to diet. Revista Brasileira de Zoologia, v. 22, No. 1, p. 181-185,
2005. https://doi.org/10.1590/S0101-81752005000100021
Cáceres, N. C.; Monteiro-Filho, E. L. A. Germination in seed species ingested by opossums:
implications for seed dispersal and forest conservation. Brazilian Archives of Biology and
Technology, v. 50, No. 6, p. 921-928, 2007. https://doi.org/10.1590/S1516-89132007000700002
Campos, W. H.; Miranda Neto, A.; Peixoto, H. J. C.; Godinho, L. B.; Silva, E. Contribuição da fauna
silvestre em projetos de restauração ecológica no Brasil. Pesquisa Florestal
Brasileira, v. 32, No. 72, p. 429-440, 2012. https://doi.org/10.4336/2012.pfb.32.72.429
Cubas, Z. S.; Silva, J. C. R.; Catão-Dias, J. L. Tratado de animais selvagens: medicina
veterinária. São Paulo: Roca, 2006.
Cunningham, J. G. Tratado de fisiologia veterinária. 3. ed. Rio de Janeiro: Guanabara
Koogan, 2004.
Delmann, H. D.; Brown, E. M. Histologia veterinária. Rio de Janeiro: Guanabara Koogan,
1982.
Eisenberg, J. F.; Redford, K. H. Mammals of the Neotropics: the Central Neotropics. Chicago:
University of Chicago Press, 2000. v. 3.
Fontes, S. V.; Passamani, M.; Jacinto, C. H.; Pereira, M. S.; Sant'ana, A. P. P. Área de vida e
deslocamento de Akodon montensis e guaiquica em um fragmento no sul de Minas Gerais. Anais do VIII
Congresso de Ecologia do Brasil, 2007.
Hildebrand, M. Análise das estruturas dos vertebrados. São Paulo: Atheneu, 1995.
Illanes, J.; Fertilio, B.; Chamblas, M.; Leyton, V.; Verdugo, F. Descripción histológica
de los diferentes segmentos del aparato digestivo de avestruz (Struthio camelus var.
domesticus). International Journal of Morphology, v. 24, No. 2, p. 205-214, 2006.
https://doi.org/10.4067/S0717-95022006000300015
Jin, S. M. Histologia do antro pilórico de Caiman crocodillus yacare (Crocodilla-Reptilla).
Revista Brasileira de Zoologia, v. 5, No. 2, p. 189-192, 1988. https://doi.org/10.1590/S0101-81751988000200002
Junqueira, L. C.; Carneiro. J. Histologia básica. 11. ed. Rio de Janeiro: Guanabara
Koogan, 2008.
Lima, J. M. N.; Santos, A. C.; Viana, D. C.; Bertassoli, B. M.; Lobo, L. M.; Oliveira, V. C.; Briani,
D. C.; Costa, G. M.; Assis-Neto, A. C.; Ambrósio, C. E.; Carvalho, A. F.; Mançanares,
C. A. F. Morphological study of the male genital organs of Gracilinanus microtarsus.
Brazilian Journal of Veterinary Research and Animal Science, v. 50, No. 6, p. 447-456,
2013. https://doi.org/10.11606/issn.1678-4456.v50i6p447-456
Lobo, L. M.; Santos, A. C.; Rosa, R. A.; Ambrósio, C. E.; Briani, D. C.; Costa, G. M.; Carvalho,
A. F.; Mançanares, C. A. F. Estudo macroscópico do aparelho digestório de Gracilinanus
microtarsus (Wagner, 1842) (Mammalia: Didelphidae). Biotemas, v. 27, No. 1, p. 109-120, 2014.
https://doi.org/10.5007/2175-7925.2014v27n1p109
Lobo, L. M.; Santos, A. C.; Rosa, R. A.; Costa, G. M.; Carvalho, A. F.; Ambrósio, C. E.; Miglino,
M. A.; Mançanares, C. A. F. Morphological and ultrastructural analysis of the tongue of
Gracilinanus microtarsus (Wagner, 1842). Bioscience Journal, v. 31, No. 6, p. 1809-1815, 2015.
https://doi.org/10.14393/BJ-v31n6a2015-30198
Lobo, L. M.; Santos, A. C.; Rosa, R. A.; Carvalho, A. F.; Costa, G. M.; Mançanares, C. A. F. Morphological
and ultrastructural analysis of the teeth of guaiquica (Gracilinanus microtarsus Wagner, 1842). Journal
of Morphological Science, v. 33, No. 2, p. 73-78, 2016. https://doi.org/10.4322/jms.089015
Martins, E. G.; Araújo, M. S.; Bonato, V.; Reis, S. F. Sex and season affect individual level diet variation
in the Neotropical Marsupial Gracilinanus microtarsus (Didelphidae). Biotropica, v. 40, No. 1,
p. 132-135, 2008. https://doi.org/10.1111/j.1744-7429.2007.00319.x
Orr, R. T. Biologia dos vertebrados. 5. ed. São Paulo: Roca, 1986.
Pires, M. M.; Martins, E. G.; Silva, M. N. F.; Reis, S. F. Gracilinanus microtarsus (Didelphimorphia:
Didelphidae). Mammalian Species, v. 42, No. 851, p. 33-40, 2010. https://doi.org/10.1644/851.1
Ribeiro, M. G. F.; Fonseca, C. C.; Sartori, S. S. R.; Loures-Ribeiro, A.; Neves, C. A. Histological description
of the small intestine of the Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae). Revista
Brasileira de Zoociências, v. 13, No. 1/3, p. 19-27, 2011. Available from: <https://zoociencias.ufjf.emnuvens.com.br/zoociencias/article/view/2502/1774.
Accessed on: Oct. 14, 2017.
Samuelson, D. A. Tratado de histologia veterinária. Rio de Janeiro: Elsevier, 2007.
Santos, D. C. M.; Cupertino, M. C.; Novaes, R. D.; Soares, I. A. C.; Fonseca, C. C.; Matta, S. L. P.;
Sartori, S. S. R. Morphologic characterization and distribution of endocrine cells in the large intestine
of the opossum Didelphis aurita (Wied-Neuwied, 1826). Tissue and Cell, v. 45, No. 5,
p. 338-349, 2013. https://doi.org/10.1016/j.tice.2013.05.003
Santori, R. T.; Astua de Morais, D.; Cerqueira, E. Comparative gross morphology of the digestive tract in
ten Didelphidae marsupial species. Mammalia, v. 68, No. 1, p. 27-36, 2004. https://doi.org/10.1515/mamm.2004.004