Vol. 5, No. 9, p. 95-104 - Apr. 30, 2018
Concurrent administration of methanolic extract of Zingiber officinale Roscoe (Zingiberales: Zingiberaceae) and diminazene aceturate enhanced survival rate and reduced parasitaemia in experimental murine Trypanosoma brucei Plimmer & Bradford, 1899 (Kinetoplastea: Trypanosomatida) infection
D. K. Anya and K. I. Eghianruwa
The efficacy of concurrent diminazene and Zingiber officinale Roscoe (Zingiberales: Zingiberaceae) extract in murine Trypanosoma brucei Plimmer & Bradford, 1899 (Kinetoplastea: Trypanosomatida) infection was evaluated. Two infected groups were treated with extract at 400 mg.kg-1 (G1) and 800 mg.kg-1 (G2) alone while another two groups received 400 mg.kg-1 (GD1) and 800 mg.kg-1 (GD2) of extract concurrently with diminazene 3.5 mg.kg-1 intraperitoneally. One infected group received diminazene 3.5 mg.kg-1 only (D) while another received 1 mg.kg-1 Tween 80 (C1) orally. The seventh group was uninfected and untreated (C2). Survival rate, parasitemia, liver weight, spleen weight, haematological indices were evaluated. Survival rates were 0% in C1, G1 and G2, 20% in D, 40% in GD2, 60% in GD1 and 100% in C2. Animals in groups G1, G2 and C1 died between 6 and 8 days pt. Parasitemia levels were significantly (P < 0.05) higher in D1 than in GD1 and GD2 by day 16 post treatment. PCV and RBC counts were significantly (P < 0.05) lower in GD1, GD2 and D than in C2. Liver and spleen weights increased significantly (P < 0.05) due to infection and never fully recovered in all treatment options. Ginger (Z. officinale) extract enhanced diminazene efficacy by increasing survival rates and lowering parasitemia.
Diminazene; Ginger; Trypanosome; Survivability; Parasitemia; Haematology; Murine.
Adeiza, A. A.; Makeri, H. K.; Mohammed, M. Comparative in vitro trypanocidal activities of water and methanol extracts of three parts of Khaya senegalensis on Trypanosoma evansi. Journal of Medicinal Plants Research, v. 4, no. 14, p. 1365-1369, 2010. https://doi.org/10.5897/JMPR10.058
Ajith, T. A.; Nivitha, V.; Usha, S. Zingiber officinale Roscoe alone and in combination with a-tocopherol protect the kidney against cisplatin-induced acute renal failure. Food Chemical Toxicology, v. 45, no. 6, p. 921 927, 2007. https://doi.org/10.1016/j.fct.2006.11.014
Akhani, S. P.; Vishwakarma, S. L.; Goyal, R. K.; Anti-diabetic activity of Zingiber officinale in streptozotocin-induced type I diabetic rats. Jounal of Pharmacy and Pharmacology, v. 56, p. 101-105, 2004. https://doi.org/10.1211/0022357022403
Benedich, A.; Olson, J. A. Biological action of carotenoids. The FASEB Jounal, v. 3, p. 1927-1932, 1989. https://doi.org/10.1096/fasebj.3.8.2656356
Blumenthal, M. Ginger as an antiemetic during pregnancy. Alternative Therapies in Health and Medicine, v. 9, p. 19-21, 2003.
Carrasco, F. R.; Schmidt, G.; Romero, A. L.; Sartoretto, J. L.; Caparroz-Assef, S. M; Bersani-Amado, C. A.; Cuman, R. K. Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syzygium aromaticum L. essential oils: evidence for humor- and cell-mediated responses. Jounal of Pharmacy and Pharmacology, v. 61, p. 961-967, 2009. https://doi.org/10.1211/jpp.61.07.0017
Chang, C. P.; Chang, J.-Y.; Wang, F.-Y.; Chang, J. G. The effect of Chinese medicinal herb Zingiberis rhizoma extract on cytokine secretion by human peripheral blood mononuclear cells. Journal of Ethnopharmacology, v. 48, p.13-19, 1995. https://doi.org/10.1016/0378-8741(95)01275-I
Eghianruwa, K. I.; Anika, S. M. Effects of dimethyl sulphoxide and ascorbic acid pretreatment on prepatent period, parasitemia, hematology and tissue pathology in rats infected with T. brucei. Tropical Veterinarian, v. 28, p. 13-20, 2010.
Eghianruwa, K. I.; Anika, S. M. The effects of selenium and tocopherol supplementation on the efficacy of diminazene aceturate in reversing T. brucei-induced anemia in rats. Veterinarski Arhiv, v. 81, p. 647-656, 2011. Available from: <https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=108648>. Accessed on: Dec. 23, 2017.
Eghianruwa, K. I. Effect of supplemental antioxidants, vitamin C and DMSO on weight gain and survivability in T. brucei infected and diminazene treated rats. Veterinarski Arhiv, v. 82, p. 519-529, 2012a. <https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=128527>. Accessed on: Dec. 23, 2017.
Eghianruwa, K. I.; Anika, S. M. Effects of DMSO on diminazene efficacy in experimental murine T. brucei infection. International Journal of Animal and Veterinary Advances, v. 4, p. 93-98, 2012b. Available from: <http://maxwellsci.com/print/ijava/v4-93-98.pdf. Accessed on: Dec. 23, 2017.
Eghianruwa, K. I.; Anika, S. M.; Akpavie, S. O. Efficacy of diminazene with and without ascorbic acid supplementation in T. brucei infected rats. Tropical Veterinarian, v. 27, p. 20-36, 2009.
Faremi, A.Y.; Ekanem, J. T. Haematological parameters and enzyme studies in Trypanosoma brucei-infected rats reared on Nigella sativa oil-based diet. Asian Journal of Biochemistry, v. 6, p. 90-97, 2011. https://doi.org/10.3923/ajb.2011.90.97
Grant, K. L.; Lutz, R. B. Ginger. American Journal of Health-System Pharmacy, v. 57, p. 945-947, 2000.
Grzanna, R.; Lindmark, L.; Frondoza, C. Ginger: An herbal medicinal product with broad anti-inflammatory action. Journal of Medical Food, v. 8, p. 125-132, 2005. https://doi.org/10.1089/jmf.2005.8.125
Haniadka, R.; Saldanha, E.; Sunita, V.; Palatty, P. L.; Fayad, R.; Baliga, M. S. A review of the gastroprotective effects of ginger (Zingiber officinale Roscoe). Food & Function, v. 4, p. 845-855, 2013. https://doi.org/10.1039/c3fo30337c
ILRI - International Livestock Research Institute. Trypanosomiasis. Available from <http://www.ilri.org/infoserv/webpub/fulldocs/ilrad87/epidemiology.htm>. Accessed on: Jul. 28, 2008.
Kaikabo, A. A.; Salako, M. A. Effects of vitamin E supplementation on anemia and tissue pathology in rats infected with Trypanosoma brucei (Federe strain). Tropical Veterinarian, v. 24, p. 46-51, 2006.
Kobo, P. I.; Erin, P. J.; Suleiman, M. M.; Aliyu, H.; Tauheed, M.; Muftau, S.; Mamman, M. Antitrypanosomal effect of methanolic extract of Zingiber officinale (ginger) on Trypanosoma brucei brucei-infected Wistar mice. Veterinary World, v. 7, p. 770-775, 2014. Available from: <http://www.veterinaryworld.org/Vol.7/October-2014/3.pdf. Accessed on: Dec. 23, 2017.
Lin, R. J.; Chen, C. Y.; Chung, L. Y.; Yen, C. M. Larvicidal activities of ginger (Zingiber officinale) against Angiostrongylus cantonensis. Acta Tropica, v. 115, p. 69-76, 2010. https://doi.org/10.1016/j.actatropica.2009.12.007
Lumb, A. B. Effect of dried ginger on human platelet function. Journal of Thrombosis and Haemostasis, v. 71, p. 110-111, 1994.
Mashhadi, N. S.; Ghiasvand, R.; Askari, G.; Hariri, M.; Darvishi, L.; Mofid, M. R. Anti-oxidative and anti-inflammatory effects of ginger in health and physical activity: Review of current evidence. International Journal of Preventive Medicine, v. 4, suppl. 1, p. S1-S7, 2013. Available from: <http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1117. Accessed on: Dec. 23, 2017.
Mojani, M. S.; Rahmat, A.; Ramasamy, R. Ginger (Zingiber officinale Roscoe) immune-nutritional behavior: An overview. Nutrition & Food Science, v. 1, suppl. 1, p. 189-190, 2014.
Naderi, Z.; Mozaffari-Khosravi, H.; Dehghan, A.; Nadjarzadeh, A.; Huseini, H. F. Effect of ginger powder supplementation on nitric oxide and C-reactive protein in elderly knee osteoarthritis patients: A 12-week double-blind randomized placebo-controlled clinical trial. Journal of Traditional and Complementary Medicine, v 28, p. 199-203, 2015. https://doi.org/10.1016/j.jtcme.2014.12.007
Ogbadoyi, E. O.; Garba, M. H.; Kabiru, A. Y.; Mann, A.; Okogun, J. Therapeutic evaluation of Acacia nilotica (Linn) stem bark extract in experimental African trypanosomiasis. International Journal of Applied Research in Natural Products, v. 4, p. 11-18, 2011. Available from: <http://www.ijarnp.org/index.php/ijarnp/article/view/6/6. Accessed on: Dec. 23, 2017.
Omoja, V. U.; Anaga, A. O.; Obidike, I. R.; Ihedioha, T. E.; Umeakuana, P. U.; Mhomga, L. I.; Asuzu, I. U.; Anika, S. M. The effects of combination of methanolic leaf extract of Azadirachta indica and diminazene diaceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats. Asian Pacific Journal of Tropical Medicine, v. 4, no. 5, p. 337-341, 2011. https://doi.org/10.1016/S1995-7645(11)60099-0
Omoya, F. O; Akharaiyi, F. C. Mixture of honey and ginger extract for antibacterial assessment on some clinical isolates. International Journal on Pharmaceutical and Biomedical Research, v. 2, no. 1, p. 39 47, 2011. Availabe from: <http://www.kejapub.com/ijpbr/docs/IJPBR11-02-01-07.pdf>. Accessed on: Dec. 23, 2017.
Onyiah, J. A. African animal Trypanosomiasis: An overview of the current status in Nigeria. Tropical Veterinarian, v. 15, p. 111-116, 1997.
Raji, Y.; Udoh, U. S.; Oluwadara, O. O; Akinsomisoye, O. S.; Awobajo, O.; Adeshoga, K. Anti-inflammatory and analgesic properties of the rhizome extract of Zingiber officinale. African Journal of Biomedical Research, v. 5, no. 3, p. 121-124, 2002. https://doi.org/10.4314/ajbr.v5i3.53999
Shiferaw, S.; Muktar, Y.; Belina, D. A review on trypanocidal drug resistance in Ethiopia. Journal of Parasitology and Vector Biology, v. 7, p. 58-66, 2015. Available from: <http://www.academicjournals.org/article/article1429095115_Shiferaw et al.pdf>. Accessed on: Dec. 23, 2017.
Shimoda, H.; Shan, S. J; Tanaka, J; Seki, A; Seo, J. W.; Kasajima, N.; Tamura, S.; Ke, Y.; Murakami, N. Anti-inflammatory properties of red ginger (Zingiber officinale var. Rubra) extract and suppression of nitric oxide production by its constituents. Journal of Medical Food, v. 13, no. 1, p. 156-162, 2010. https://doi.org/10.1089/jmf.2009.1084
Shirin, A. P. R.; Prakash, J. Chemical composition and antioxidant properties of ginger root (Zingiber officinale). Journal of Medicinal Plants Research, v. 4, no. 24, p. 2674-2679, 2010. Available from: <http://www.academicjournals.org/article/article1380710823_Shirin and Jamuna.pdf>. Accessed on: Dec. 23, 2017.
Stohs, S. J.; Hartman, M. J. Review of the safety and efficacy of Moringa oleifera. Phytotherapy Research, v. 29, p. 796-804, 2015. https://doi.org/10.1002/ptr.5325
Surh, Y. J.; Park, K. K.; Chun, K. S.; Lee, L. J.; Lee, E.; Lee, S. S. Anti-tumor-promoting activities of selected pungent phenolic substances present in ginger. Journal of Environmental Pathology, Toxicology and Oncology, v. 18, p. 131-139, 1999.
Toma, I.; Shinggu, D. Y.; Ezekiel, W.; Barminas, J. T. Effect of intraperitoneal administration of vitamin C (ascorbic acid) on anemia in experimental Trypanosoma congolense infected rabbits. African Journal of Pure Applied Chemistry, v. 2, p. 37-40, 2008.
Vutyavanich, T.; Kraisarin, T.; Ruangsri, R. Ginger for nausea and vomiting in pregnancy: randomized, double-masked, placebo-controlled trial. Obstetrics & Gynecology, v. 97, no. 4, p. 577-582, 2001. Available from: <https://journals.lww.com/greenjournal/Fulltext/2001/04000/Ginger_for_Nausea_and_Vomiting_in_Pregnancy_.17.aspx. Accessed on: Dec. 23, 2017.
Yu, B. P. Cellular defenses against damage from reactive oxygen species. Physiological Reviews, v. 74, no. 1, p. 139-162, 1994. https://doi.org/10.1152/physrev.19184.108.40.206