Validation of cleaning procedures and sanitization of equipment in the fish industry by microbiological analysis

Hélen Baptista Abud , Erick Eduardo da Silveira , Elaine Berges da Silva , Antônio Francisco da Silva Lisboa-Neto , Amilton Cesar dos Santos and Caio Biasi

Abstract
Data by the Pan-American Health Organization show that the death of about two million people a year and over 200 types of diseases are associated with increasing food contamination. Several types of technology and extensive legislation have been developed and targeted to this issue to reduce food microbiological load to safer levels and meet the expectations of increasingly demanding consumers. Current research investigates the process of sanitization of equipment and utensils in the fish industry by microbiological analyses. The study was conducted in a fish industry, installed in São Roque, State of São Paulo, Brazil. Swab technique was employed prior to cleaning and after sanitizing. Microbiological analysis comprised total counts of coliforms, fecal coliforms, psychrotrophic bacteria, mesophylls and Pseudomonas. In present study, microbiological analysis available in compendium of methods for the microbiological examinations of foods were useful for establish possible contamination by microorganisms. Correct sanitization throughout the manufacturing process has been targeted to maintain the quality and safety of the final product. Satisfactory results of present study may contribute to the discussion about standardization of sanitary procedures for fishing industry. Further research on the fishery industry should be undertaken to establish reliable standards to be employed nationwide.

Keywords
Coliforms; Food-borne diseases; Food quality; Pathological microorganism; Pseudomonas.

DOI
10.21472/bjbs.050907

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References
Brasil. Agência Nacional de Vigilância Sanitária. Resolução RDC n^o 12, de 2 de janeiro de 2001. Available from: <http://portal.anvisa.gov.br/documents/33880/2568070/RDC_12_2001.pdf/15ffddf6-3767-4527-bfac-740a0400829b>. Accessed on: Feb. 23, 2018
Instrução Normativa n^o 62, de 20 de setembro de 2011. Brasília: Ministério da Agricultura, Pecuária e Abastecimento, 2011.

Franco, B. D. G. M.; Landgraf, M. Microbiologia de alimentos. São Paulo: Atheneu, 1996.

Germano, P. M. L.; Germano, M. I. S. Higiene e vigilância sanitária de alimentos: qualidade das matérias-primas, doenças transmitidas por alimentos, treinamento de recursos humanos. Barueri: Manole, 2015.

ISO - International Organization for Standardization. ISO 13720:2010 Meat and meat products - Enumeration of presumptive Pseudomonas spp. Available from: <https://www.iso.org/obp/ui/#iso:std:iso:13720:ed-2:v1:en>. Accessed on: Feb. 23, 2018.

ISO - International Organization for Standardization. ISO 4833-1:2013 Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique. Available from: <https://www.iso.org/standard/53728.html>. Accessed on: Feb. 23, 2018.

Lima, C. L. S. Avaliação dos perigos microbiológicos em uma indústria de beneficiamento de pescado e sugestão de um sistema de gestão de qualidade. Belém: Universidade Federal do Pará, 2012. (Thesis).

Ogawa, M.; Maia, E. L. Manual da pesca: ciência e tecnologia do pescado. São Paulo: Varela, 1999.

Salfinger, Y.; Tortorello, M. L. Compendium of methods for the microbiological examination of foods. 5. ed. New York: American Public Health Association, 2015.

Silva, N. Manual de métodos de análise microbiológica de alimentos e águas. 4. ed. São Paulo: Varela, 2010.

Silva-Jr., E. A. Manual de controle higiênico-sanitário em serviços de alimentação. 7. ed. São Paulo: Varela, 2014.