Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 5, no. 9 (2018) Abioye


Vol. 5, No. 9, p. 25-32 - Apr. 30, 2018


Biosorption of lead, chromium and cadmium in tannery effluent using indigenous microorganisms

O. P. Abioye , O. A. Oyewole , S. B. Oyeleke , M. O. Adeyemi , A. A. Orukotan

This study investigated the biosorption of lead, chromium and cadmium in tannery effluent using indigenous microorganisms. Bacteria isolated from the tannery effluent were Bacillus subtilis and B. megaterium while fungi isolated were Aspergillus niger and Penicillium sp. The microorganisms were tested for their ability to reduce the concentration of the heavy metals in the tannery effluent using conventional methods. B. megaterium recorded the highest lead reduction (2.13 to 0.03 mg/L), followed by B. subtilis (2.13-0.04 mg/L). A. niger recorded the highest ability to reduce the concentration of chromium (1.38-0.08 mg/L) followed by Penicillium sp. (1.38 0.13 mg/L) while B. subtilis exhibited the highest ability to reduce the concentration of cadmium (0.4-0.03 mg/L) followed by B. megaterium (0.04-0.06 mg/L) after 20 days. When these values were compared to standard limits of Federal Environmental Protection Agency (FEPA), World Health Organization (WHO), National Environmental Standard and Regulations Enforcement Agency (NESREA) and Central Pollution Control Board (CPCB), the isolates recorded an acceptable reduction in the concentration of lead, chromium and cadmium in sterile and unsterile tannery effluent. The results of this showed that the isolates reduced the concentration of lead, chromium and cadmium present in the sterile and raw tannery effluent and suggest that the organisms can be used as a possible treatment of tannery effluents.

Tannery effluent; Lead; Chromium; Cadmium; Biosorption; Bacteria; Fungi.


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Ain, R.; Tash, J. S.; Soares, M. J. Prolactin-like protein-A is a functional modulator of natural killer cells at the maternal-fetal interface. Molecular and Cellular Endocrinology, v. 204, no. 1/2, p. 65-74, 2003.

Abioye, O. P.; Adefisanm A. E.; Aransiola, S. A.; Damisa, D. Biosorption of chromium by Bacillus subtilis and Pseudomonas aeruginosa isolated from waste dumpsite. Expert Opinion on Environmental Biology, v. 4, No. 1, p. 1-3, 2015.

Akar, T.; Tunali, S. Biosorption characteristic of Aspergillus flavus biomass for removal of Pb (II) and Cu (II) ions from an aqueous solution. Bioresource Technology, v. 97, p. 1780-1787, 2006.

Bello, O. A.; Abubakar, B. Y.; Abdullahi, I. O. Efficiency of Aspergillus niger, Aspergillus flavus and Microsporum nanum to remove heavy metals from refinery effluent. Journal of Advances in Biology and Biotechnology, v. 6, No. 3, p. 1-6, 2016.

Bosnic, M.; Buljan, J.; Daniels, R. P. Pollutants in tannery effluents. Vienna: United Nations Industrial Development Organization, 2000. Available from: < tannery_effluents_0.pdf>. Accessed on: Nov. 24, 2017.

Cheesbrough, M. District Laboratory Practice in Tropical Countries. London: Cambridge University Press, 2006. Part 2.

Devi, R.; Arasappan, S.; Kalyanaraman, R.; Narasimhan, B. A study on biosorption potential of Aspergillus sp. of tannery effluent. Advances in Bioscience and Biotechnology, v. 5, p. 853-860, 2011.

Deivasgamani, C.; Das, N. Biodegradation of basic violet 3 by Candida krusel isolated from textile waste water. Biodegradation, v. 22, No. 6, p. 1169-1180, 2011.

Do, T.; Shen, J.; Cawood, G.; Jeckins, R. Biotreatment of textile effluent using Pseudomonas spp. immobilized on polymer supports. In: Hardin, I. R.; Akin, D. E.; Wilson, J. S. (Eds.). Advances in biotreatment for textile processing. Georgia: University of Georgia Press, 2002. p. 234-256.

Domsch, K. H.; Gams, W. Fungi in agricultural soils. 1. ed. London: Longman, 1970.

Ezzouhri, L.; Castro, E.; Moya, M.; Espionola, F.; Lairini, K. Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. African Journal of Microbiology Research, v. 3, No. 2, p. 35-48, 2009. Available from: < et al.pdf>. Accessed on: Nov. 24, 2017.

Hakeem, A. S.; Bhatnagar, S. Heavy metal reduction pulp and paper mill effluent by indigenous microbes. Asian Journal of Experimental Biological Sciences, v. 1, p. 201-203, 2010. Available from: < of Applied Scienc201-203.pdf>. Accessed on: Nov. 24, 2017.

Holt, J. G;. Krieg, N. R.; Sneath, P. H. A.; Staley, J. T.; Williams, S. T. Bergy's manual of determinative Bacteriology. Maryland: Williams and Wilkins Publishers, 1994.

Jayanthi, M.; Kanchana, D.; Saranraj, P.; Sujitha, D. Bioadsorption of chromium by Penicillium chrysogenum and Aspergillus niger isolated from tannery effluent. International Journal of Microbiological Research, v. 5, No. 1, p. 40-47, 2014.

Konopka, A.; Zakharova, T. Qualification of bacterial lead resistance via activity assays. Journal of Microbiological Methods, v. 37, No. 1, p. 17-22, 1999.

León, M.; Yaryura, P. M.; Montecchia, M. S.; Hernández, A. I.; Correa, O. S.; Pucheu, N. L.; Kerber, N. L.; García, A. F. Antifungal activity of selected indigenous Pseudomonas and Bacillus from soyabean rhizosphere. International Journal of Microbiology, v. 2009, Article ID 572049, p. 1-9, 2009.

Maier, J.; Kandelbauer, A.; Erlacher, A.; Cavaco-Paulo, A.; Gubits, G. M. A new alkali-thermostable azoreductase from Bacillus sp. strain SF. Applied Environmental Microbiology, v. 70, p. 837-844, 2004.

Malarkodi, M.; Krishnasamy, R.; Kumaraperumal, R.; Chitdeshwari, T. Characterization of heavy metal contaminated soils of Coimbatore District in Tamil Nadu. Journal of Agronomy, v. 6, No. 1, p. 147-151, 2007.

Meenambigai, P.; Vijayaraghavan, R.; Shyamala, G. R.; Rajarajeswari, P.; Prabhavathi, P. Biodegradation of heavy metals: a review. International Journal Current Microbiology Applied Science, v. 5, No. 4, p. 375-383, 2016.

Mustafa, S.; Ahmed, T.; Naum, A.; Shah, K. H.; Wassum, M. Kinetics of chromium ion removal from tannery wastes using Amberliti IRA-400 Cl- and its hybrids. Water, Air, Soil, & Pollution, v. 210, No. 1/4, p. 43-50, 2010.

Noorjahan, C. Physicochemical characteristics, identification of fungi and biodegradation of industrial effluent. Journal of Environment and Earth Science, v. 4, No. 4, p. 32-39, 2014. Available from: <>. Accessed on: Nov. 24, 2017.

Olukanni, O. D.; Osuntoki, A. A.; Gbenle, G. O. Textile effluent biodegradation potentials of textile effluent-adapted and non-adapted bacteria. African Journal of Biotechnology, v. 5, No. 20, p. 1980-1984, 2006. Available from: <>. Accessed on: Nov. 24, 2017.

Rabah, A. B.; Ibrahim, M. L. Physicochemical and microbiological characterization of soils laden with tannery effluents in Sokoto, Nigeria. Nigerian Journal of Basic and Applied Science, v. 18, No. 1, p. 65-71, 2010. Available from: <>. Accessed on: Nov. 24, 2017.

Salman, H. A.; Ibrahim, M. I.; Tarek, M. M.; Abbas, H. S. Biosorption of heavy metals: a review. Journal of Chemical Science and Technology, v. 3, No. 4, p. 74-102, 2014. Available from: <>. Accessed on: Nov. 24, 2017.

Santhi, R.; Guru, V. Biosorption of hexavalent chromium using Aspergillus niger dead biomass and its optimization studies. International Journal of Current Microbiology and Applied Science, v. 3, No. 10, p. 669-678, 2014. Available from: < and V.Guru.pdf. Accessed on: Nov. 24, 2017.

Srinivas, G.; Boominathan; Estari, M. Studies of efficiency of immobilised bacteria in tannery effluent treatment. Journal of Biology Innovation, v. 2, No. 2, p. 33-42, 2013. Available from: <>. Accessed on: Nov. 24, 2017.

Shazia, I.; Kousar, P.; Naila, S.; Kanwal, W.; Ijaz, A.; Iftikhar, A. Tolerance potential of different species of Aspergillus as bioremediation tool. Comparative analysis. E3 Journal of Microbiology Research, v. 1, No. 1, p. 1-8, 2013. Available from: < galley proof_gagz-1.pdf>. Accessed on: Nov. 24, 2017.

Shivakumar, C. K.; Thippeswamy, B. Effect of fungal biosorbed and nonbiosorbed copper and zinc metal solution on growth and metal uptake of leguminous plans. Research Journal, v. 2, No. 2, p. 6 12, 2012. Available from: <>. Accessed on: Nov. 24, 2017.

Verma, T.; Garg, S. K.; Ramteke, P. W. Genetic correlation between chromium resistance and reduction in Bacillus brevis isolated from tannery effluent. Journal of Applied Microbiology, v. 107, No. 5, p. 1425-1432, 2009.