Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 5, no. 10 (2018) Jayakrishnan


Vol. 5, No. 10, p. 427-432 - Aug. 31, 2018


A facile approach towards copper oxide nanoparticles synthesis using Spirulina platensis and assessment of its biological activities

Priyanga Jayakrishnan , Sirajunnisa Abdul Razack , Keerthana Sivanesan , Pavithra Sellaperumal , Geethalakshmi Ramakrishnan , Sangeetha Subramanian and Renganathan Sahadevan

There is a budding need to develop a method for environmentally benign metal nanoparticle synthesis, that do not use toxic chemicals in the synthesis protocols to avoid adverse effects in medical applications. The present investigation dealt with the synthesis of copper oxide (CuO) nanoparticles from blue green alga, Spirulina platensis. The algal extract consisting of phytochemicals was used as the reducing agent and copper sulphate as the substrate. Synthesised nanoparticles were characterized by UV-Vis spectrophotometry, FT-IR spectroscopy, XRD and SEM. Antibacterial and anticancer activities were assessed for the CuO nanoparticles. The results indicated that the formed CuO nanoparticles were observed to be nanosheets. FT-IR spectral analysis elucidated the occurrence of biomolecules required for the reduction of copper oxide ions. The synthesized nanoparticles were found to be effective at the concentration of 1 mg/mL against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Serratia marcescens. The cytotoxicity activity of CuO nanoparticle was evaluated by MTT Assay against colon cancer cell lines and confirmed that CuO nanoparticle at a concentration of 125 µg/mL had cytotoxic activity. In conclusion, the CuO nanoparticles were synthesized at a low energy supply, in an ecologically safe mode which could be utilized for pharmacological applications and various biotechnological studies.

Spirulina platensis; Copper nanoparticles; Well diffusion; Colon cancer; MTT assay.


Full text

Azam, A.; Ahmed, A. S.; Oves, M.; Khan, M. S.; Habib, S. S.; Memic, A. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: A comparative study. International Journal of Nanomedicine, v. 2012, p. 6003-6009, 2012.

Cuevas, R.; Durán, N.; Diez, M. C.; Tortella, G. R.; Rubilar, O. Extracellular biosynthesis of copper and copper oxide nanoparticles by Stereum hirsutum, a native white-rot fungus from Chilean forests. Journal of Nanomaterials, v. 2015, Article ID 789089, 2015.

Dallas, P.; Sharma, V. K.; Zboril, R. Silver polymeric nanocomposites as advanced antimicrobial agents: Classification, synthetic paths, applications, and perspectives. Advances in Colloid and Interface Science, v. 166, no. 1, p. 119-135, 2011.

Gabbay, J.; Borkow, G.; Mishal, J.; Magen, E.; Zatcoff, R.; Shemer-Avni, Y. Copper oxide impregnated textiles with potent biocidal activities. Journal of Industrial Textiles, v. 35, no. 4, p. 323-335, 2006.

Grigore, M. E.; Biscu, E. R.; Holban, A. M.; Gestal, M. C.; Grumezescu, A. M. Methods of synthesis, properties and biomedical applications of CuO nanoparticles. Pharmaceuticals, v. 9:75, 2016.

Hajipour, M. J.; Fromm, K. M.; Ashkarran, A. A.; Aberasturi, D. J.; de Larramendi, I. R.; Rojo, T.; Mahmoudi, M. Antibacterial properties of nanoparticles. Trends in Biotechnology, v. 30, n. 10, p. 499-511, 2012.

Hetta, M.; Mahmoud, R.; El-Senousy, W.; Ibrahim, M.; El-Taweel, G.; Ali, G. Antiviral and antimicrobial activities of Spirulina platensis. World J. Pharm. Pharm. Sci., v. 3, no. 6, p. 31-39, 2014.

Jain, S.; Hirst, D. G.; O’Sullivan, J. M. Gold nanoparticles as novel agents for cancer therapy. The British Journal of Radiology, v. 85, no. 1010, p. 101-113, 2012.

Jeronsia, J. E.; Raj, D. V.; Joseph, L. A.; Rubini, K.; Das, S. J. In vitro antibacterial and anticancer activity of copper oxide nanostructures in human breast cancer Michigan Cancer Foundation-7 cells. Journal of Medical Sciences, v. 36, no. 4, 145-151, 2016.

Kanmani, P.; Yuvaraj, N.; Paari, K. A.; Pattukumar, V.; Arul, V. Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro. Bioresource Technology, v. 102, no. 7, p. 4827-4833, 2011.

Nagajyothi, P. C.; Muthuraman, P.; Sreekanth, T. V. M.; Kim, D. H.; Shim, J. Green synthesis: In-vitro anticancer activity of copper oxide nanoparticles against human cervical carcinoma cells. Arabian Journal of Chemistry, v. 10, no. 2, p. 215-225, 2017.

Nguyen, K. T. Targeted nanoparticles for cancer therapy: Promises and challenge. Journal of Nanomedicine & Nanotechnology, 2:5, 2011.

Niraimathi, K. L.; Lavanya, R.; Sudha, V.; Narendran, R.; Brindha, P. Bio-reductive synthesis and characterization of copper oxide nanoparticles (CuONPs) using Alternanthera sessilis Linn. leaf extract. Journal of Pharmacy Research, v. 10, no. 1, p. 29-32, 2016.

Padil, V. V. T.; Cerník, M. Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application. International Journal of Nanomedicine, v. 8, p. 889-898, 2013.

Patel, V.; Berthold, D.; Puranik, P.; Gantar, M. Screening of cyanobacteria and microalgae for their ability to synthesize silver nanoparticles with antibacterial activity. Biotechnology Reports, v. 5, p. 112-119, 2015.

Rai, M.; Posten, C. (Eds.). Green biosynthesis of nanoparticles: Mechanisms and applications. CABI, 2013.

Sankar, R.; Maheswari, R.; Karthik, S.; Shivashangari, K. S.; Ravikumar, V. Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Materials Science and Engineering: C, v. 44, p. 234-239, 2014.

Shafagh, M.; Rahmani, F.; Delirezh, N. CuO nanoparticles induce cytotoxicity and apoptosis in human K562 cancer cell line via mitochondrial pathway, through reactive oxygen species and P53. Iranian Journal of Basic Medical Sciences, v. 18, no. 10, p. 993 1000, 2015.

Suganya, K. U.; Govindaraju, K.; Kumar, V. G.; Dhas, T. S.; Karthick, V.; Singaravelu, G.; Elanchezhiyan, M. Blue green alga mediated synthesis of gold nanoparticles and its antibacterial efficacy against Gram positive organisms. Materials Science and Engineering: C, v. 47, p. 351-356, 2015.

Verma, S. U. G. A. N. D. H. A.; Kumari, B. A. B. I. T. A.; Shrivastava, J. N. Green synthesis of silver nanoparticles using single cell protein of Spirulina platensis. International Journal of Pharma and Bio Sciences, v. 5, no. 2, p. B458-B464, 2014.

Yang, J.; Jiang, L. C.; Zhang, W. D.; Gunasekaran, S. A highly sensitive non-enzymatic glucose sensor based on a simple two-step electrodeposition of cupric oxide (CuO) nanoparticles onto multi-walled carbon nanotube arrays. Talanta, v. 82, no. 1, p. 25-33, 2010.