Vol. 5, No. 10, p. 417-426 - Aug. 31, 2018
In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)
Samanjit Kaur , M. Syed Ali , V. Anuradha , V. Suganya , A. Ashashalini and P. Bhuvana
In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.
Bioactivity; Marine halophytes; Anti-inflammation activity; Lysosomal enzymes.
Anuradha, V.; Suganya, V.; Syed Ali, M.; Sangeetha, P.; Bhuvana, P. In vitro anti-diabeticactivity of microencapsulated and non-encapsulated Astaxanthin. International Journal of Current Pharmaceutical Research, v. 9, no. 5, p. 90-96, 2017. Available from: <https://innovareacademics.in/journals/index.php/ijcpr/article/view/22147>. Accessed on: Apr. 23, 2018.
Banerjee, D.; Chakrabarti, S.; Hazra, A. K.; Banerjee, S.; Ray, J.; Mukherjee, B. Antioxidant activity and total phenolics of some mangroves in Sundarbans. African Journal of Biotechnology, v. 7, no. 6, p. 805-810, 2008. Available from: <https://www.ajol.info/index.php/ajb/article/view/58545/46890>. Accessed on: Apr. 23, 2018.
Chou, C. T. The antiinflammatory effect of Tripterygium wilfordii Hook F on adjuvant-induced paw oedema in rats and inflammatory mediators release. Phytotherapy Research, v. 11, no. 2, p. 152-154, 1997. https://doi.org/10.1002/(SICI)1099-1573(199703)11:2<152::AID-PTR45>3.0.CO;2-L
Kathiresan, K.; Bingham, B. L. Biology of mangroves and mangrove ecosystems. Advances in Marine Biology, v. 40, p. 81-251, 2001. https://doi.org/10.1016/S0065-2881(01)40003-4
Manilal, A. An in vitro antagonistic efficacy validation of Rhizophora mucronata. Asian Pacific Journal of Tropical Disease, v. 5, no. 1, p. 28-32, 2015. https://doi.org/10.1016/S2222-1808(14)60622-8
Ravikumar, S.; Inbaneson, S. J.; Suganthi, P. In vitro antiplasmodial activity of chosen terrestrial medicinal plants against Plasmodium falciparum. Asian Pacific Journal of Tropical Biomedicine, v. 2, no. 1, Suppl., p. S252-S256, 2012. https://doi.org/10.1016/S2221-1691(12)60169-5
Ravikumar, S.; Muthuraja, M.; Sivaperumal, P.; Gnanadesigan, M. Antibacterial activity of the mangrove leaves Exoecaria agallocha against selected fish pathogens. Asian Journal of Medical Sciences, v. 2, no. 5, p. 211-213, 2010. Available from: <http://maxwellsci.com/print/ajms/v2-211-213.pdf>. Accessed on: Apr. 23, 2018.
Sree Kumari, C.; Yasmi, N.; Raffiq Hussain, M.; Babuselvam, M. In vitro anti-inflammatory and anti-arthritic property of Rhizopora mucronata leaves. International Journal of Pharma Sciences and Research, v. 6, no. 3, p. 482-485, 2015. Available from: <http://www.ijpsr.info/docs/IJPSR15-06-03-052.pdf. Accessed on: Apr. 23, 2018.
Syed Ali, M.; Vijaya, P. P.; Wilson, D.; Aswan Ali, A.M.; Yogananth, N.; Anuradha, V.; Kalitha Parveen, P. Evaluation of in-vitro anti-inflammatory and antimicrobial properties of Pergularia daemia and Solanum xanthocarpum. International Journal of Current Microbiology and Applied Sciences, v. 2, no. 1, p. 94-99, 2013. Available from: <https://www.ijcmas.com/Archives/vol-2/PDF/P.P. Vijaya, et al.pdf>. Accessed on: Apr. 23, 2018.
Vadivu, R.; Lakshmi, K. S. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp laurina. Bangladesh Journal of Pharmacology, v. 3, no. 2, p. 121-124, 2008. https://doi.org/10.3329/bjp.v3i2.956