Vol. 5, No. 10, p. 359-372 - Aug. 31, 2018
GCMS, phytochemicals and antioxidant activities of in vitro callus extracts of Strobilanthes kunthiana (Nees) T. Anderson ex Benth: An endemic plant of Acanthaceae
R. Prabakaran and E. Kirutheka
Abstract
Strobilanthes kunthiana (Neelakurinji) is an endemic and underexploited plant belongs to the Family Acanthaceae. The aim of our study was to evaluate phytochemical analysis, GC-MS analysis and antioxidant activity of in vitro callus extract of Strobilanthes kunthiana. In this present study the phytochemical analysis of various extract of S. kunthiana in vitro callus were studied. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, steroids, terpenoids and phenols in the methanolic extract comparing to the other extracts. In this study GC-MS analysis revealed the presence of 10 bioactive phytochemical compounds were identified in the methanolic extract. The prevailing compounds were 9,12-octadecadienoic acid (Z,Z) (50.32%), hexadecanoic acid, methyl ester (20.69%), 9-octadecenoic acid (Z)-,methyl ester (10.45%), heptadecanoic acid, 16-methyl-, methyl ester (5.78%), 2,6-bis(1,1-dimethylethyl)-4-methyl phenol (4.70%). 3-methyl-2-ketobutyric acid tbdms (2.79%), 2,2,3,4-tetramethyl-5-hexen-3-ol (2.41%), N-(tert-butoxycarbonyl)-2-(4-methoxyphenyl) allylamine (1.06%), cyclotrisiloxane, hexamethyl (0.94%), benzenesulfonamide (0.87%). The antioxidant property was evaluated for methanol and ethanolic extract by DPPH method. The higher percentage of inhibition (79.23 + 0.37) was observed in 250 μg/mL of ethanol extract followed by (90.35 + 0.54) methanolic extract against the standard ascorbic acid (91.25 + 0.33). The results show that the methanolic extract possesses more antioxidant activity than ethanol. The plant S. kunthiana may be exploited as a source of natural antioxidant and as herbal alternatives for various disorders.
Keywords
In vitro callus; Phytochemical; GC-MS analysis; DPPH; Antioxidant.
DOI
10.21472/bjbs.051015
Full text
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References
Andrews, K T.; Fisher, G. M.; Sumanadasa, S. D.; Skinner-Adams, T.; Moeker, J.; Lopez, M.;
Poulsen, S. A. Antimalarial activity of compounds comprising a primary benzene sulfonamide
fragment. Bioorganic & Medicinal Chemistry Letters, v. 23, no. 22, p. 6114-6117,
2013. https://doi.org/10.1016/j.bmcl.2013.09.015
Anonymous I. Indian pharmacopoeia. Delhi: Government of India, Ministry of Health
and Welfare, Controller of Publications, 1985.
Anonymous II. Quality control methods of Medicinal plant materials. Geneva: WHO,
Delhi: A.I.T.B.S Publishers & Distributors, 2002.
Arora, S.; Kumar, D.; Shiba. Phytochemical, antimicrobial and antioxidant activities of
methanol extract of leaves and flowers of Ipomoea cairica. International Journal
of Pharmacy and Pharmaceutical Sciences, v. 5, no.1, p. 198-202, 2013.
Asghar, S. F.; Habib-ur-Rehman; Choudahry, M. I.; Atta-ur-Rahman. Gas chromatography-mass
spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bio-assays of crude
extract of Iris germanica. International Journal of Genetics and Moleular Biology,
v. 3, no. 7, p. 95-100, 2011.
Augustine, J.; Josekutty, E. J.; Biju, P. Strobilanthes sainthomiana: A new species of
Strobilanthes Blume (Acanthaceae) from Western Ghats, India. Taiwania,
v. 62, no. 1, p. 63-66, 2017.
Ayoola, G. A.; Coker, H. A. B.; Adesegun, S. A.; Adepoju-Bello, A. A.; Obaweya, K.; Ezennia,
E. C.; Atangbayila, T. O. Phytochemical screening and antioxidant activities of some selected
medicinal plants used for malaria therapy in Southwestern Nigeria. Tropical Journal of
Pharmaceutical Research, v. 7, no. 3, p. 1019-1024, 2008. https://doi.org/10.4314/tjpr.v7i3.14686
Balandrin, M. F.; Kinghorn, A. D.; Farnsworth, N. R. Plant-derived natural products in drug
discovery and development: An overview. In: Kinghorn, A D.; Balandrin, M. F. Human medicinal
agents from plants. Washington, DC: American Chemical Society, 1993. (ACS Symposium Series).
p. 2-12. https://doi.org/10.1021/bk-1993-0534.ch001
Barakat, M. Z.; Shahab, S. K.; Darwin, N.; Zahemy, E. I. Determination of ascorbic acid from
plants. Annal of Biochem., v. 53, p. 225-245, 1993.
Belakhdar, G.; Benjouad, A.; Abdennebi, E.H. Determination of some bioactive chemical
constituents from Thesium humile Vahl. J. Mater. Environ. Sci., v. 6,
no. 10, p. 2778-2783, 2015.
Belkacem, N.; Djaziri, R.; Lahfa, F.; El-Haci, I. A.; Boucherit, Z. Phytochemical screening
and in vitro antioxidant activity isolated bioactive compounds from Tridax procumbens
Linn. Pakistan Journal of Biological Sciences, v. 16, no. 24, p. 1971-1977, 2013. https://doi.org/10.3923/pjbs.2013.1971.1977
Brand-Williams, W.; Cuvelier, M. E.; Berset, C. Use of free radical method to evaluate antioxidant
activity. Lebensmittel Wissenschaft und Technologie, v. 28, p. 25-30, 1995. https://doi.org/10.1016/S0023-6438(95)80008-5
Cai, Y. Z.; Sun, M. Antioxidant activity of betalins from plants of the Amaranthacea. Journal of
Agriculture and Food Chemistry, v. 51, no. 8, p. 2288-2294, 2003. https://doi.org/10.1021/jf030045u
Chandrasekaran, M.; Senthilkumar, A.; Venkatesalu, V. Antibacterial and antifungal ef?cacy of fatty
acid methyl esters from leaves of Sesuvium portulacastrum L. Eur. Rev. Med. Pharmcol. Sci.,
v. 15, no. 7, p. 775-780, 2011.
Desu, B. S. R.; Elango, K.; Satish Kumar, M. N.; Suresh, B.; Manimaran. S.; Nanjan, M. J. In-vitro
anti-inflammatory and anti-osteoarthritic activities of Strobilanthes kunthianus and Strobilanthes
cuspidatus. International Journal of Research in Pharmaceutical and Biomedical Sciences, v. 1,
no. 4, p. 2231-2781, 2011.
Desu, B. S. R.; Elango, K.; Satish Kumar, M. N.; Suresh, B.; Manimaran, S.; Nanjan, M. J. Analgesic activity
of Strobilanthes kunthianus and Strobilanthes cuspidatus. International Journal of Research
in Pharmaceutical and Biomedical Sciences, v. 3, no. 1, p. 2229-3701, 2012.
Elaiyaraja, A.; Chandramohan, G. Comparative phytochemical profile of Crinum defixum Ker-Gawler leaves
using GC-MS. Asian Journal of Pharmaceutical Research and Development, v. 4, no. 3, p. 1-13, 2016.
https://doi.org/10.18052/www.scipress.com/ILNS.46.8
Epand, R. F.; Savage, P. B.; Epand, R. M. Bacterial lipid composition and the antimicrobial efficacy of
cationic steroid compounds (Ceragenins). Biochemica et Biophysica Acta (BBA) - Biomembranes,
v. 1768, no. 10, p. 2500-2509, 2007. https://doi.org/10.1016/j.bbamem.2007.05.023
Everlyne, I. M.; Darsini, D. T. P.; Yadav, S. A. Unraveling antibiofilm potency of Strobilanthes
kunthiana Nees T. Anderson ex Benth against throat-infectious methicillin-resistant
Staphylococcus aureus. Indo American Journal of Pharmaceutical Research, v. 6, no. 6,
p. 5707-5716, 2016.
Everlyne, I. M.; Sangilimuthu, A. Y.; Darsini, D. T P. Spectral analyses of the bioactive compounds
present in the ethanolic leaf extract of Strobilanthes kunthiana (Nees) T. Anderson ex
Benth. Advances in Bioresearch, v. 6, no. 3, p. 65-71, 2015.
Gibbs, R. D. Chemotaxonomy of flowering plants. Montreal: McGill Queen's University Press,
1974. v. 1. https://doi.org/10.2307/j.ctt1w0ddx8
Guptha, A. K.; Neeraj, T.; Sharma, M. Quality standards of Indian Medicinal Plants. ICMR, v. 3,
p. 9 19, 2005.
Halliwell, B.; Gutteridge, J. M. Free radicals in Biology and Medicine. Oxford: Oxford University
Press, 1999.
Han, X.; Shen, T; Lou, H. Dietary polyphenols and their biological significance. Int. J. Mol. Sci.,
v. 8, no. 9, p. 950-988, 2007. https://doi.org/10.3390/i8090950
Hema, R.; Kumaravel, S.; Alagusundaram, K. GC/MS determination of bioactive components of Murraya
koenigii. Journal of American Science, v. 7, no. 1, p. 80-83, 2011.
Hites, R. Gas chromatography mass spectroscopy. In: Settle, F. A. (Ed.). Handbook of instrumental
techniques for analytical chemistry. Upper Saddle River, NJ: Prentice Hall PTR, 1997.
Ibtissem, B.; Imen, M.; Souad, S. Dosage of 2, 6-bis (1.1-dimethylethyl)-4-methyl phenol (BHT) in the
plant extract Mesembryanthemum crystallinum. Journal of Biomedicine and Biotechnology,
v. 2010, Article ID 142486, 5 p., 2010. https://doi.org/10.1155/2010/142486
Jayasri, M. A.; Mathew, L.; Radha, A. A report on the antioxidant activities of leaves and rhizomes
of Costus pictus D. Don. International Journal of Integretive Biology, v. 5, no. 1,
p. 20-26, 2009.
Kar, A. Pharmaocgnosy and pharmacobio-technology. 2. ed. New Delhi: New Age International
Limited Publishers, 2007.
Krishnamoorthy, K.; Subramaniam, P. Phytochemical profiling of leaf, stem, and tuber parts of
Solena amplexicaulis (Lam.) Gandhi using GC-MS. International Scholarly Research
Notices, v. 2014, Article ID 567409, 13 p., 2014. https://doi.org/10.1155/2014/567409
Madsen, H. L.; Bertelsen, G. Spices as antioxidants. Trends Food Science and Technology,
v. 6, p. 271-277, 1995. https://doi.org/10.1016/S0924-2244(00)89112-8
Madziga, H. A.; Sanni, S.; Sandabe, U. K. Phytochemical and elemental analysis of Acalypha
wilkesiana leaf. Journal of American Science, v. 6, no. 11, p. 510-514, 2010.
Mangunwidjaja, D. S.; Kardono, S. R.; Iswantini, L. B. S. D. Gas chromatography and gas
chromatography-mass spectrometry analysis of Indonesian Croton tiglium seeds. J.
Applied Sci., v. 6, no. 7, p. 1576-1580, 2006. https://doi.org/10.3923/jas.2006.1576.1580
Motar, M. L. R.; Thomas, G.; Barbosa Filho, J. M. Effects of Anacardium occidentale
stem bark extract on in vivo inflammatory models. J. Ethnopharm., v. 95,
no. 2/3, p. 139-142, 1985.
Moylan, E. C.; Bennett, J. R.; Carine, M. A.; Olmstead, R. G.; Scotland, R. W. Phylogenetic
relationships among Strobilanthes (Acanthaceae): Evidence from ITS nrDNA, trnL-F cpDNA,
and morphology. American Journal of Botany, v. 91, no. 5, p. 724-735, 2004. https://doi.org/10.3732/ajb.91.5.724
Paulsamy, S.; Vijayakumar, K. K.; Murugesan, M.; Padmavathy, S.; Senthilkumar, P. Ecological
status of medicinal and other economically important plants in the shola understories of Nilgiris,
the Western Ghats. Natural Products Radiance, v. 6, no. 1,p. 55-61, 2007.
Peach, K.; Tracey, M. V. Modern methods of plant analysis. Berlin: Springer Verlag, 1956. v. 3.
Peter, P. J.; Venky, V. M. Phytochemical and GC-MS studies Onindigofera linnaei Linn. International
Journal of Phytopharmacy Research, v. 2, no. 5, p. 143-148, 2012. https://doi.org/10.7439/ijpp.v2i5.615
Rabi, T.; Bishayee, A. Terpenoids and breast cancer chemoprevention. Breast Cancer Research and
Treatment, v. 115, no. 2, p. 223-239, 2009. https://doi.org/10.1007/s10549-008-0118-y
Rajasekaran, A.; Loganathan, V.; Jaewanth, A.; Jayakar, B. Central nervous activity of Strobilanthes
kunthiana leaf. Hamdard Medicus, v. 43, no. 1, p. 38-40, 2000.
Rajeswari, B.; Srinivasan, M. GC-MS analysis of bioactive components from the ethanolic leaf extract of
Flueggea leucopyrus Wild. International Journal of Pharmaceutical Sciences Review and Research,
v. 33, no. 1, p. 270-273, 2015.
Rajeswari, G.; Murugan, M.; Mohan, V. R. GC-MS analysis of bioactive components of Hugonia mystax L.
(Linaceae). Research Journal of Pharmaceutical, Biological and Chemical Sciences, v. 3, no. 4,
p. 301-308, 2012.
Rajeswari, J.; Rani, S. GC-MS analysis of phytochemical compounds in the ethanolic extract of root of
Lawsonia inermis Linn. International Journal of ChemTech Research, v. 7, no. 1,
p. 389-399, 2015.
Ruch, R. J.; Cheng, S. J.; Klaunig, J. E. Prevention of cytotoxicity and inhibition of Intercellular
communication by antioxidant catechins isolated from Chinese green tea. Carcinogens, v. 10,
p. 1003-1008, 1989. https://doi.org/10.1093/carcin/10.6.1003
Selvan, P. S.; Velavan, S. Analysis of bioactive compounds in methanol extract of Cissus vitiginea
leaf using GC-MS technique. Rasayan Journal of Chemistry, v. 8, no. 4, p. 443-447, 2015.
Shah, B. A.; Qazi, G. N.; Taneja, S. C. Boswellic acids: a group of medicinally important compounds.
Natural Product Report, v. 26, p. 72-89, 2009. https://doi.org/10.1093/carcin/10.6.1003
Singh, B. B.; Das, S.; Maithi, A. Antioxidant Property for lipophilic extract of Strobilanthes
kunthiana flowers. Indian Journal of Research in Pharmacy and Biotechnology, v. 2,
no. 1, p. 1005-1009, 2014.
Singh, D. N.; Verma, N.; Kulshreshtha, D. K.; Agrawal, A. K. In-vitro antigiardial activity of
ethanolic extract and fractions from Phlebophyllum kunthianum. Journal of Natural Remedies,
v. 12, no. 1, p. 68-71, 2012.
Singh, R.; Singh, S. K.; Arora, S. Evaluation of antioxidant potential of ethyl acetate extract/fractions
of Acacia auriculiformis. A. Cunn. Food Chem. Toxicol., v. 45, no. 7, p. 1216-1223, 2007.
Sirappuselvi, S.; Chitra, M. In vitro antioxidant activity of Cassia tora Lin. International
Research Journal of Biological Sciences, v. 1, no. 6, p. 57-61, 2012.
Treare, G. E.; Evans, W. C. Pharmacognosy. 16. ed. London: Bahive Tinal, 1985. https://doi.org/10.1016/B978-0-7020-2933-2.00002-2
Uddin, S. N.; Akond, M. A.; Mubassara, S.; Yesmin, M. N. Antioxidant and antibacterial activities of
Trema cannabina. Middle-East Journal of Scientific Research, v. 3, no. 2, p. 105-108, 2008.
Venkatesh, R.; Vidya, R.; Kalaivani, K. Gas chromatography and mass spectrometry analysis of Solanum
villosum (Mill) (Solanaceae). International Journal of Pharmaceutical Sciences and Research,
v. 5, no. 12, p. 52-83, 2014.
Vetha Merlin Kumari, H.; Manickavasakam, K.; Mohan, S. GC-MS analysis of bioactive components of a siddha
poly herbal drug Adathoda chooranam. Int. J. RES. Ayurveda Pharm., v. 7, no. 2, 2016. https://doi.org/10.7897/2277-4343.07245
Wagner, K. H.; Elmadfa, I. Biological relevance of terpenoids: overview focusing on mono-, di- and
tetraterpenes. Ann. Nutr. Metab., v. 47, p. 95-106, 2003. https://doi.org/10.1159/000070030
Yu, B. P. Cellular defenses against damage from reactive oxygen species. Physiological Reviews, v. 74,
no. 1, p. 139-162, 1994. https://doi.org/10.1152/physrev.1994.74.1.139