Nutritional and environmental factors affecting the morphogenesis of Candida albicans: A key to virulence

Sayyada Ghufrana Nadeem and Aiman Pirzada

Abstract
Candida albicans is an opportunistic fungal pathogen that plays an important role in the early part of infectious process by extravagating and disseminating to the target organs, whereas hyphal forms appear to be required for the mortality resulting from a deep-seated infection. C. albicans morphogenesis is regulated by numerous environmental cues and other signaling pathways. We investigated the morphogenesis in C. albicans in the presence of serum at different temperatures (20 ^oC, 30 ^oC and 37 ^oC). C. albicans were also grown in simple growth medium 'SDB' and subsequently cultured from Fetal bovine serum and Soybeans dextrose broth (SDB) on Sabouraud dextrose agar, Yeast extract potato dextrose agar and Spider medium. The combination of serum and temperature is excellent at promoting the yeast to mycelial conversion and it also induces the expression of hyphal specific genes. Our results demonstrate that the combination of serum and each temperature provides a distinct proportion of blastospores, budding yeast cells, germ tube, pseudohyphae and true hyphal cells. Remarkable change in colonial pattern between the cells cultured after incubating in serum and the cells cultured after incubating in SDB was observed in SDA in contrast with YEPD and Spider medium. On most solid media, colonies of C. albicans are composed of three types of cells: budding yeast, pseudohyphae and hyphae. All three forms are also found in infected tissues, and the transition between these forms is a key for pathogenesis.

Keywords
Candida albicans; Virulence; Spider medium; Serum; Temperature.

DOI
10.21472/bjbs.051011

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References
Barelle, C. J.; Bohula, E. A.; Kron, S. J.; Wessels, D.; Soll, D. R.; Schafer, A.; Brown, A. J.; Gow, N. A. Asynchronous cell cycle and asymmetric vacuolar inheritance in true hyphae of C. albicans. Eukaryotic Cell, v. 2, p. 398-410, 2003.

Barlow, A. J. E.; Aldersley, T.; Chattaway, F. W. Factors present in serum and seminal plasma which promote germ-tube formation and mycelia growth of C. albicans. Microbiology, v. 82, p. 261-272, 1974.

Berman, J.; Sudbery, P. E. C. albicans: A molecular revolution built on lessons from budding yeast. Nature Reviews Genetics, v. 3, p. 918-930, 2002.

Brice, E.; Malcolm, W. Release from Quorum-Sensing Molecules triggers hyphal formation during C. albicans resumption of growth. Eukaryotic Cell, v. 4, p. 1203-1210, 2005.

Brown, D. H. J. R.; Giusani, A. D.; Chen, X.; Kumamoto, C. A. Filamentous growth of C. albicans in response to physical environmental cues and its regulation by the unique CZF1 gene. Molecular Microbiology, v. 34, p. 651-662, 1999.

Buffo, J.; Herman, N.; Soll, D. R. A characterization of pH regulated dimorphism in C. albicans. Mycopathologia, v. 85, p. 21-30, 1985.

Bachewich, C.; Malcolm, W. Cyclin Cln3p Links G1 Progression to Hyphal and Pseudohyphal Development in C. albicans. Eukaryotic Cell, v. 4, p. 95-102, 2005.

Dalle, F.; Wachtler, B.; L'Ollivier, C.; Holland, G.; Bannert, N.; Wilson, D.; Labruere, C.; Bonnin, A.; Hube, B. Cellular interactions of C. albicans with human oral epithelial cells and enterocytes. Cellular Microbiology, v. 12, p. 248-271, 2010.

David, K.; Alexander, D. J. Induction of the C. albicans filamentous growth program by relief of transcriptional repression: A genome-wide analysis. Molecular Biology of the Cell, v. 16, p. 2903-2912, 2005.

Feng, Q.; Summers, E.; Guo, B.; Fink, G. Ras signaling is required for serum-induced hyphal differentiation in C. albicans. Journal of Bacteriology, v. 181, p. 6339-6346, 1999.

Gow, N. A.; Brown, A. J.; Odds, F. C. Fungal morphogenesis and host invasion. Current Opinion in Microbiology, v. 5, p. 366-371, 2002.

Gow, N. A. R. Cell biology and cell cycle of Candida. In: Calderone, R. A. (Ed.). Candida and Candidiasis. Washington, D.C.: ASM Press, 2002. p. 145-158.

Hazen, K. C.; Hazen, B. W. Temperature-modulated physiological characteristics of C. albicans. Microbiology and Immunology, v. 31, p. 497-508, 1987.

Homann, O. R.; Dea, J.; Noble, S. M.; Johnson, A. D. A Phenotypic Profile of the C. albicans Regulatory Network. PLoS Genetics, v. 5, e1000783, 2009.

Hornby, J. M.; Dumitru, R.; Nickerson, K. W. High phosphate (up to 600mM) induces pseudohyphal development in five wild type C. albicans. Journal of Microbiological Methods, v. 56, p. 119-124, 2003.

Hudson, D. A.; Sciascia, Q. L.; Sanders, R. J.; Norris, G. E.; Edwards, P. J.; Sullivan, P. A.; Farley, P. C. Identification of the dialyzable serum inducer of germ-tube formation in C. albicans. Microbiology, v. 150, p. 3041-3049, 2004.

Lee, K. L.; Buckley, H. R.; Cambell, C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of C. albicans. Sabouraudia: Journal of Medical and Veterinary Mycology, v. 13, p. 148-153, 1975.

Liu, H. P.; Kohler, J. R.; Fink, G.R. Suppression of hyphal formation in C. albicans by mutation of a STE12 homolog. Science, v. 266, p. 1723-1726, 1994.

Maaroufi, Y.; De Bruyne, J.M.; Duchateau, V.; Georgala, A.; Crokaert, F. Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay. The Journal of Molecular Diagnostics, v. 6, p. 108-114, 2004.

Mardon, D.; Balish, E.; Phillips, A.W. Control of dimorphism in a biochemical variant of C. albicans. Journal of Bacteriology, v. 100, p. 701–707, 1969.

Molero, G.; Diez Orejas, R.; Navarro Garcia, F.; Monteoliva, L.; Pla, J.; Gil, C.; Sanchez Perez, M.; Nombela, C. C. albicans: genetics, dimorphism and pathogenicity. International Microbiology: The Official Journal of The Spanish Society for Microbiology, v. 1, p. 95-106, 1998.

Navarro, G. F.; Sanchez, M.; Nombela, C.; Pla, J. Virulence genes in the pathogenic yeast C. albicans. FEMS Microbiology Reviews, v. 25, p. 245-268, 2001.

Odds, F. C. Candida and Candidosis. London: Balliere Tindall, 1988.

Pesti, M.; Sipiczki, M.; Pinter, Y. Scanning electron microscopy characterization of colonies of C. albicans morphological mutants. Journal of Medical Microbiology, v. 48, p. 167-172, 1999.

Shareck, J.; Belhumeur, P. Modulation of morphogenesis in C. albicans by various small molecules. Eukaryotic Cell, v. 10, p. 1004-1012, 2011.

Simonneti, N.; Stripolli, V.; Cassone, E.A. Yeast-mycelial conversion induced by N-acetyl-d-glucosamine in C. albicans. Nature, v. 250, p. 344-346, 1974.

Sudbery, P.; Gow, N.; Berman, J. The distinct morphogenic states of C. albicans. Trends in Microbiology, v. 12, p. 317-324, 2004.

Taschdjian, C. L.; Burchall, J. J.; Kozinn, P. J. Rapid identification of C. albicans by filamentation on serum and serum substitutes. AMA Journal of Diseases of Children, v. 99, p. 212-215, 1960.

Yang, L.; Chang, S.; Wang, A.; Liu, H. Hyphal Development in C. albicans requires Two Temporally Linked Changes in Promoter Chromatin for Initiation and Maintenance. PLoS Biology, v. 9, e1001105, 2011.