Molecular Era of the Forensic Science

Yorran Hardman A. Montenegro , Stephanie Evelyn França Guimarães , Estefany Gabriela Luiz de Araújo , Ros´lia Santos Ferreira , Ellen Rachel de Almeida Martins , Maria Karoline da Silva Marcelino , Denise de Queiroga Nascimento and Geilza Carla de Lima Silva

Abstract
Forensic science is an interdisciplinary area that is intimately related between the fields of physics, biology, chemistry, mathematics and forensic opportunity sciences, with the aim of supporting criminal investigations and civil justice. Forensic Entomology enters as the most promising and attention-grabbing course, especially for its versatility in identifying morphological characteristics in species identification, requiring new analytical tools such as microcopy and molecular analysis. With regard to the Molecular Age in Forensic Science, new technologies can be applied in the research field regarding necrobiota, a new and promising molecular tool that indicates the possible places of displacement of the body and people involved; phenotyping as an analytical tool for the phenotypic identification of the possible appearance of the suspect or missing person; epigenetics in the identification of suspects in the case of homozygous twins. All new tools open up the numerous possibilities for further development of Forensic Science, allowing greater agility in resolving criminal investigations.

Keywords
Phenotyping; Necrobiota; Epigenetics; COI-I.

DOI
10.21472/bjbs.051001

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References
Alves, A. C. F.; Santos, W. E.; Farias, R. C. A. P.; Creão-Duarte, A. J. Blowfliers (Diptera, Calliphoridae) associated with pig carcasses in a Caatinga area, Northeastern Brazil. Neotropical Entomology, v. 43, n. 2, p. 122-126, 2014. https://doi.org/10.1007/s13744-013-0195-4

Carriço, C.; Mendonça, P. M.; Cortinhas, L. B.; Mallet, J. R.; Queiroz, M. M. Ultrastructural studies of some character of Diptera (Muscidae) of forensically importance. Acta Tropical, v. 142, p. 96-102, 2014. https://doi.org/10.1016/j.actatropica.2014.11.005

Chaitanya, L.; Pajnič, I. Z.; Walsh, S.; Balažic, J.; Zupanc, T.; Kayser, M. Bringing colour back after 70 years: Predicting eye and hair colour from skeletal remains of World War II victims using the HIrisPlex System. Forensic Science International: Genetics, v. 26, p. 48-57, 2017. https://doi.org/10.1016/j.fsigen.2016.10.004

Fagertun, J. Wolffhechel, K.; Pers, T.H.; Nielsen, H. B.; Gudbjartsson, D.; Stefansson, H.; Stefansson, K.; Paulsen, R. R.; Jarmer, H. Predicting facial characteristics from complex polygenic variations. Forensic Science International: Genetics, v. 19, p. 263-268, 2015. https://doi.org/10.1016/j.fsigen.2015.08.004

Finley, S. J.; Benbow, M. E.; Javan, G. T. Potential applications of soil microbial ecology and next-generation sequencing in criminal investigations. Applied Soil Ecology, v. 88, no. 1, p. 69-78, 2015. https://doi.org/10.1016/j.apsoil.2015.01.001

Hebert, P. D. N.; Gregory, T. R. The promise of DNA barcoding for taxonomy. Systematic Biology, v. 54, n. 5, p. 852-859, 2005.

Iqbal, M. A.; Katie, D.; Maiken, N. U.; Forbes, S. L. Forensic decomposition odour profiling: A review of experimental designs and analytical techniques. TrAC Trends in Analytical Chemistry, v. 91, p. 112-124, 2017. https://doi.org/10.1016/j.trac.2017.04.009

Kader, F.; Ghai, M. DNA methylation and application in forensic sciences. Forensic Science International, v. 249, p. 255-265, 2015. https://doi.org/10.1016/j.forsciint.2015.01.037

Kayser, M. Forensic DNA Phenotyping: Predicting human appearance from crime scene material for investigative purposes. Forensic Science International: Genetics, v. 18, p. 33-48, 2015. https://doi.org/10.1016/j.fsigen.2015.02.003

Lee, H. Y.; Jung S. E.; Oh, Y. N.; Choi, A.; Yang, W. I.; Shin K. J. Epigenetic age signatures in the forensically relevant body fluid of semen: a preliminary study. Forensic Science International: Genetics, v. 19, p. 28-34, 2015. https://doi.org/10.1016/j.fsigen.2015.05.014

Mądra, A.; Frątczak, K.; Grzywacz, A.; Matuszewski, S. Long-term study of pig carrion entomofauna. Forensic Science International, v. 1, no. 252, p. 1-10, 2015.

Maroñas, O.; Phillips, C.; Söchtig, J.; Gomez-Tato, A.; Cruz, R.; Alvarez-Dios, J.; Casares de Cal, M.; Ruiz, Y.; Fondevila, M..; Carracedo, A.; Lareu, M. V. Development of a forensic skin colour predictive test. Forensic Science International: Genetics, v. 13, p. 34-44, 2014. https://doi.org/10.1016/j.fsigen.2014.06.017s

Pechal, J. L.; Crippen, T. L.; Benbow, M. E.; Tarone, A. M.; Dowd, S.; Tomberlin, J. K. The potential use of bacterial community succession in forensics as describedby high throughput metagenomic sequencing. International Journal of Legal Medicine, v. 128, no. 1, p. 193-205, 2014. https://doi.org/10.1007/s00414-013-0872-1

Pentinsaari, M.; Mutanen, M.; Kaila L. Cryptic diversity and signs of mitochondrial introgression in the Agrilusviridis species complex (Coleoptera: Buprestidae). European Journal of Entomology, v. 1, no. 111, p. 475-486, 2014. https://doi.org/10.14411/eje.2014.072

Samerjai, C.; Sanit, S.; Sukontason, K.; Morakote, N.; Wannasan, A.; Pereira, R. M.; Sukontason, K. L. Morphology of immature stages of flesh flies, Boettcherisca nathani and Lioproctia pattoni (Diptera: Sarcophagidae). Acta Tropical, v. 163, p. 109-120, 2016. https://doi.org/10.1016/j.actatropica.2016.08.008

Sharma, R.; Garg, R. K.; Gaur, Jr. Various methods for the estimation of the postmortem interval from Calliphoridae: A review. Egyptian Journal of Forensic Sciences, v. 5, no. 1, p. 1-12, 2013. https://doi.org/1 0.1016/j.ejfs.2013.04.002

Szpila, K.; Mądra, A.; Jarmusz, M.; Mateuszewski, S. Flesh flies (Diptera: Sarcophagidae) colonising large carcasses in Central Europe. Parasitology Research, v. 114, no. 114, p. 2341-2348, 2015. https://doi.org/10.1007/s00436-015-4431-1

Tsang, L. M.; Chu, K. H.; Nozawa, Y.; Chan, B. K. K. Morphological and host specificity evolution in coral symbiont barnacles (Balanomorpha: Pyrgomatidae) inferred from a multi-locus phylogeny. Molecular Phylogenetic Evolution, v. 1, no. 77, p. 11 22, 2014. https://doi.org/10.1016/j.ympev.2014.03.002

Tuccia, F.; Giordani, G.; Vanin, S. A general review of the most common COI primers for Calliphoridae identification in forensic entomology. Forensic Science International: Genetics, v. 24, no. 1, p. 9-11, 2016. https://doi.org/10.1016/j.fsigen.2016.07.003

Tuccia, F.; Giordani, G.; Vanin, S. Epigenetic discrimination of identical twins from blood under the forensic scenario. Forensic Science International: Genetics, v. 31, p. 67-80, 2017a. https://doi.org/10.1016/j.fsigen.2017.07.014

Vidaki, A.; Ballard, D.; Aliferi, A.; Miller, T. H.; Barron, L. P.; Court, D. S. DNA methylation-based forensic age prediction using artificial neural networks and next generation sequencing. Forensic Science International: Genetics, v. 28, p. 225-236, 2017. https://doi.org/10.1016/j.fsigen.2017.02.009

Virmond, M. B.; Robert, A. W.; Brito, P. B.; Massuda, T. Y. C. Fenotipagem forense pelo DNA através de SNPs. Revista Brasileira de Criminalística, v. 5, no. 2, p. 37-47, 2016. https://doi.org/10.15260/rbc.v5i2.128

Walsh, S.; Wollstein, A.; Liu, F.; Chakravarthy, U.; Rahu, M.; Seland, J. H.; Soubrane, G.; Tomazzoli, L.; Topouzis, F.; Vingerling, J. R.; Vioque, J.; Fletcher, A. E.; Ballantyne, K. N.; Kayser, M. DNA-based eye colour prediction across Europe with the IrisPlex system. Forensic Science International: Genetics, v. 6, no. 3, p. 330-340, 2012. https://doi.org/10.1016/j.fsigen.2011.07.009

Zajac, B. K.; Martin-Vega, D.; Feddern, N.; Fremdt, H.; Castro, C. P.; Szpila, K.; Reckel, F.; Schutt, S.; Verhoff, M. A.; Amendt, J.; Zehner, R. Molecular identification and phylogenetic analysis of the forensically important Family Piophilidae (Diptera) from different European locations. Forensic Science International, v. 259, no. 1, p. 77-84, 2016. https://doi.org/10.1016/j.forsciint.2015.12.024

Zanetti, N. I.; Visciarelli, E. C.; Centeno, N. D. Marks caused by the scavenging activity of Necrobia rufipes (Coleoptera: Cleridae) under laboratory conditions. Journal of Forensic and Legal Medicine, v. 33, p. 116-120, 2015. https://doi.org/10.1016/j.jflm.2015.04.020