Brazilian Journal of Biological Sciences (ISSN 2358-2731)



Home Archive v. 4, no. 8 (2017) Echi

 

Vol. 4, No. 8, p. 317-321 - Dec. 31, 2017

 

Glycogen and protein profiles of Clinostomum tilapiae Ukoli, 1966 (Digenea: Clinostomidae) and putative species Clinostomum Morphotype1 in a tropical river (Anambra River Basin, Otuocha, Nigeria)



Paul Chinedu Echi

Abstract
There has been little attention to the glycogen and protein profiles of Clinostomum tilapiae Ukoli, 1966 (Digenea: Clinostomidae) and recently studied Clinostomum Morphotype1 as well as the epidemiological status of the later. The protein content of the cyst membrane 10.10 + 0.90 g/dL and host muscle tissue 11.9 + 0.80 g/dL are higher than the protein content of Clinostomum Morphotype1 tissue 7.9 + 0.40 g/dL. Conversely, the host muscle tissue glycogen content 8.25 + 1.07 mg/kg is slightly lower than both the cyst tissue 9.20 + 0.54 mg/kg and Morphotype1 9.06 + 0.79 mg/kg, respectively. However, the glycogen content of Clinostomum tilapiae 13.10 + 0.92 mg/kg is higher than both host muscle tissue glycogen content 8.25 + 1.07 mg/kg and the cyst tissue 9.20 + 0.54 mg/kg. Clinostomum tilapiae exhibited the invasive characteristic of trematodes. Season influenced the occurrence of Clinostomum Morphotype1 with higher prevalence in dry (54.01%) than rainy season months (45.98%).


Keywords
Trematoda; Clinostomum tilapiae; Clinostomum Morphotype1; Glycogen; Protein; Seasonal variation.

DOI
10.21472/bjbs.040810

Full text
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References
Bush, A. O.; Lafferty, K. D.; Lotz, J. M.; Shostak, A. W. Parasitology meets ecology on its own terms: Margolis et al. revisited. The Journal of Parasitology, v. 83, p. 575-583, 1997. https://doi.org/10.2307/3284227

Caffara, M.; Locke, S. A.; Echi, P. C.; Halajian, A.; Benini, D.; Luus-Powell, W. J.; Tavakol, S.; Fioravanti, M. L. A morphological and molecular study of clinostomid metacercariae from African fish with a redescription of Clinostomum tilapiae. Parasitology, v. 144, no. 11, p. 1519-1529, 2017. https://doi.org/10.1017/S0031182017001068

Echi, P. C.; Ezenwaji, H. M. G. The parasites fauna of characids' (Osteichthyes: Characidae) Anambra River, Nigeria. African Journal of Ecology, v. 48, p. 1-4, 2010. https://doi.org/10.1111/j.1365-2028.2009.01030.x

Echi, P. C.; Iyaji, F. O.; Ejere, V. C.; Abuh, S. J. Dynamics of synchronized clinostomatids infections in cichlids. Environment Conservation Journal, v. 15, no. 1/2, p. 49-54, 2014. Available from: <http://environcj.in/uploads/2014/12/Echi,P.C._ 15(1&2)_2014_49-54.pdf>. Accessed on: Apr. 25, 2017.

Echi, P. C.; Ojebe, M. O. Differences in host-infection patterns of clinostomatids (Clinostomatidae) in human disturbed aquatic ecosystems against undisturbed lake. Brazilian Journal of Biological Sciences, v. 3, no. 5, p. 97-103, 2016. https://doi.org/10.21472/bjbs.030508

Echi, P. C.; Suresh, K. U.; George, S.; Ratheesh, R. V.; Ezeonu, I. M.; Ejere, V. C.; Eyo, J. E.; Nwani, C. D. Molecular resolution of some West African Birds using DNA barcoding. Environment Conservation Journal, v. 16, no. 1/2, p. 87-92, 2015. Available from: <http://www.environcj.in/uploads/2015/12/87-92.pdf>. Accessed on: Apr. 25, 2017.

Higgins, J. C. The role of the tegument of the metacercarial stage of Bucephalus haimeanus (Lacaze-Duthiers, 1854) in the absorption of particulate material and small molecules in solution. Parasitology, v. 78, no. 1, p. 99-106, 1979. https://doi.org/10.1017/S0031182000048629

Jadhav, B. V.; Singh Shivesh, P.; Bhure, D. B.; Padwal, N. D. Biosystematics studies of Davainea shindei n. sp. (Cestoda: Davainidae) Fuhrmann, 1907 from Gallus gallus domesticus. National Academy Science Letters, v. 31, n. 7/8, p. 245-250, 2008.

Kemp, A.; Van Hejnigen, M. K. A colorimetric micromethod for the determination of glycogen in tissues. Biochemestry Journal, v. 56, p. 646 652, 1954. Available from: <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1269684/pdf/biochemj01086-0132.pdf>. Accessed on: Apr. 25, 2017.

Lowry, O. H.; Rosebrough, N. C.; Farr, A. L.; Randall, R. J. Protein measurements with folin-phenol reagent. Journal of Biological Chemistry, v. 193, p. 265-275, 1951. Available from: <http://www.jbc.org/content/193/1/265.full.pdf>. Accessed on: Apr. 25, 2017.

Nanware, S. S.; Bhure, D. B. Studies on glycogen profile of cestodes of Capra hircus. International Multidisciplinary Research Journal, v. 1, no. 10, p. 22-24, 2011. Available from: <https://scienceflora.org/journals/index.php/imrj/article/view/2688/2666>. Accessed on: Apr. 25, 2017.

Olaosebikan, B. D.; Raji, A. Field guide to Nigerian freshwater fishes. New Bussa: Federal College of Freshwater Fisheries Technology, 1998.

Ramanaiah, B. V.; Agarwal, S. M. Quantitative studies of glycogen in Clinostomum complanatum (Rud. 1809) and Euclinostomum heterostomum (Rud. 1809) (Clinostomatidae: Trematoda) and a note on the correlation between the total glycogen and the habitat of worms. Indian Journal of Experimental Biology, v. 13, n. 2, p. 221-222, 1975.

Shareef, P. A.; Abidi, S. Incidence and histopathology of encysted progenetic metacercaria of Clinostomum complanatum (Digenea: Clinostomidae) in Channa punctatus and its development in experimental host. Asian Pacific Journal of Tropical Biomedicine, v. 2, no. 6, p. 421-426, 2012. https://doi.org/10.1016/S2221-1691(12)60068-9

Sonune, M. B. Glycogen content of some fish parasites (Cestodes) from West Coast of India. Bioscience Discovery, v. 5, n. 1, p. 32-34, 2014. Available from: http://biosciencediscovery.com/Vol 5 No 1 Jan 2014/M B Sonune32-34.pdf>. Accessed on: Apr. 25, 2017.

Violante-González, J.; Rojas-Herrera, A.; Aguirre-Macedo, M. L. Seasonal patterns in metazoan parasite community of the "Fat Sleeper" Dormitator latifrons (Pisces: Eleotridae) from Tres Palos Lagoon, Guerrero, Mexico. Revista de Biología Tropical, v. 56, no. 3, p. 419-1427, 2008. Available from: <https://revistas.ucr.ac.cr/index.php/rbt/article/download/5719/5461>. Accessed on: Apr. 25, 2017.