Vol. 4, No. 8, p. 307-316 - Dec. 31, 2017
Analysis of biochemical and nutritional constituents of different size groups of Macrobrachium malcolmsonii (Milne-Edwards, 1844) (Decapoda: Palaemonidae) for the identification of its nutritional requirements
Thirumurugan Ramasamy , Arun Sridhar , Rajkumar Krishnasamy Sekar and Sathya Deepika Murugesan
Prawns have been contributing almost 15% by value in global aquaculture production. In this study, different size groups (4-19 cm) of fresh water prawn, Macrobrachium malcolmsonii (Milne-Edwards, 1844) (Decapoda: Palaemonidae) were collected from the Cauvery River, Tamil Nadu, India, and estimated to identify the protein (P), lipid (L) and carbohydrate (CHO) level. The adult prawns exhibited highest values 61.63% P, 6.95% L and 5.40% CHO. The gas chromatography of small size groups of prawns showed increased amount in various fatty acids such as palmitic acid, stearic acid etc. The tissues result determined by high performance liquid chromatography showed that the amino acids such as Aspartic acid, Lysine etc., are high in adult groups. The protein profile of the muscle samples displayed various polypeptides ranging from 200 to 20 kDa. Results of this study clearly implied the biochemical and nutritional constituents of freshwater prawn, M. malcolmsonii. It would be useful to design the required composition of feed in order to get higher yield at lower cost production.
Macrobrachium malcolmsonii; SDS-PAGE; HPLC; Fatty acid profile; Gas chromatography.
Alava, V.; Pascual, F. Carbohydrate requirements of Penaeus monodon (Fabricius) Juveniles. Aquaculture, v. 61, no. 3/4, p. 211 217, 1987. https://doi.org/10.1016/0044-8486(87)90150-5
Augusto, A.; Masui, D. C. Sex and reproductive stage differences in the growth, metabolism, feed, fecal production, excretion and energy budget of the Amazon River prawn (Macrobrachium amazonicum). Marine and Freshwater Behavior and Physiology, v. 47, p. 373-388, 2014.
Bhavan, P. S.; Radhakrishnan, S.; Seenivasan, C.; Shanthi, R.; Poongodi, R.; Kannan, S. Proximate composition and profiles of amino acids and fatty acids in the muscle of adult males and females of commercially viable prawn species Macrobrachium rosenbergii collected from natural culture environments. International Journal of Biology, v. 2, p. 108-119, 2010.
Carter, C. G.; Mente, E. Protein synthesis in crustaceans: a review focused on feeding and nutrition. Central European Journal of Biology, v. 9, no. 1, p. 1-10, 2013. https://doi.org/10.2478/s11535-013-0134-0
Correia, A.; Costa, M.; Luis, O.; Livingstone, D. Age-related changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in whole body Gammarus locusta (Crustacea: Amphipoda). Journal of Experimental Marine Biology and Ecology, v. 289, p. 83-101, 2003.
Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebers, P. A.; Smith, F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, v. 28, p. 350-356, 1956.
Ekpenyong, E.; Williams, I.; Osakpa, U. Variation in the proximate, energy and mineral compositions of different body parts of Macrobrachium macrobranchion (Prawn). Journal of Food Research, v. 2, p. 150-156, 2013. https://doi.org/10.5539/jfr.v2n2p150
FAO - Food and Agriculture Organization of the United Nations. The state of world fisheries and aquaculture. Rome: FAO Fisheries and Aquaculture Department, 2012.
Farmanfarmaian, A.; Lauterio, T. Amino acid composition of the tail muscle of Macrobrachium rosenbergii-comparison to amino acid patterns of supplemented commercial feed pellets. Proceedings of the World Mariculture Society, v. 11, p. 454-462, 1980. https://doi.org/10.1111/j.1749-7345.1980.tb00140.x
Folch, J.; Less, M.; Sloane Stanley, G. H. A simple method for isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, v. 226, p. 497-509, 1957. Available from: <http://www.jbc.org/content/226/1/497>. Accessed on: May 22, 2017.
Gamble, S.; Pirozzi, I.; Hall, M.; Zeng, C.; Conlan, J.; Francis, D. The effects of pre-digested protein sources on the performance of early–mid stage Panulirus ornatus phyllosoma. Aquaculture, v. 440, p. 17-24, 2015. https://doi.org/10.1016/j.aquaculture.2015.01.024
Hill, C.; Quigley, M.; Cavaletto, J.; Gordon, W. Seasonal changes in lipid content and composition in the benthic amphipods Monoporeia affinis and Pontoporeia femorata. Limnology and Oceanography, v. 37, p. 1280-1289, 1992. https://doi.org/10.4319/lo.1922.214.171.1240
Hird, F. J. The importance of arginine in evolution. Comparative Biochemistry and Physiology, Part B, v. 85, p. 285-288, 1986. https://doi.org/10.1016/0305-0491(86)90001-5
Joshi, V. P.; Diwan, A. D. Biochemical changes in the tissues of female prawn Macrobrachium idella, during different breeding seasons. Journal of Aquaculture in the Tropics, v. 113, p. 227-251, 1996.
Kanaujia, D. R.; Mohanty, A. N.; Tripathi, S. D. Growth and production of Indian river prawn Macrobrachium malcolmsonii (H. Milne Edwards) under pond conditions. Aquaculture, v. 154, p. 79-85, 1997. https://doi.org/10.1016/S0044-8486(97)00013-6
Kaushik, S.; Seiliez, I. Protein and amino acid nutrition and metabolism in fish: current knowledge and future needs. Aquaculture Research, v. 41, p. 322-332, 2010. https://doi.org/10.1111/j.1365-2109.2009.02174.x
Konosu, S.; Yamaguchi, K. The flavor components in fish and shellfish. In: Martin, R. E.; Flick, G. J.; Ward, D. R. (Eds.). Chemistry and Biochemistry of Marine Food Products. Westport, CT: AVI, 1982. p. 367-404.
Laemmli, U. Changes of structural proteins during the assembly of the head of bacteriophage T4. Nature, v. 227, p. 680-685, 1970.
Laxmappa, B.; Savalla, M. K. Polyculture of the freshwater prawn Macrobrachium malcolmsonii (H. M. Edwards) in Koilsagar Reservoir of Mahabubnagar District (TS), India. International Journal of Fisheries and Aquatic Studies, v. 2, p. 147-152, 2015. Available from: <http://www.fisheriesjournal.com/archives/2015/vol2issue4/PartC/2-4-14.pdf. Accessed on: May 22, 2017.
Lowry, O. H.; Rosenbrough, N. J.; Fair, A. L.; Randall, R. J. Protein measurement with folin phenol reagent. Journal of Biological Chemistry, v. 193, p. 265-275, 1951. Available from: <http://www.jbc.org/content/193/1/265. Accessed on: May 22, 2017.
Miller, L.; Berger, T. Bacteria identification by gas chromatography of whole cell fatty acids. Hewlett-Packard Application Note, p. 228-241, 1985.
New, M. B. Farming freshwater prawn: a manual for the culture of the giant river prawn (Macrobrachium rosenbergii). FAO Fisheries Technical Paper, v. 428, p. 212, 2002.
Nor Faadila, M. I.; Harivaindaran, K.V.; Tajul, A. Y. Biochemical and texture property changes during molting process of tiger prawn, Penaeus monodon. International Food Research Journal, v. 20, p. 751-758, 2013. Available from: <http://www.ifrj.upm.edu.my/20 (02) 2013/34 IFRJ 20 (02) 2013 Tajul (168).pdf>. Accessed on: May 23, 2017.
Pan, B. S.; Yeh, W. T. Biochemical and morphological changes in the grass shrimp Penaeus monodon muscle following freezing by air blast and liquid nitrogen methods. Journal of Food Biochemistry, v. 17, p. 147-160, 1993. https://doi.org/10.1111/j.1745-4514.1993.tb00464.x
Rosa, R.; Costa, P. R.; Bandarra, N.; Nunes, M. L. Changes in tissue biochemical composition and energy reserves associated with sexual maturation in the ommastrephid squids Iiex coindetii and Todaropsis eblanae. Biological Bulletin, v. 208, p. 100-113, 2005.
Sarac, Z.; Thaggard, H.; Saunders, J.; Gravel, M.; Neil, A.; Cowan, R. T. Observations on the chemical composition of some commercial prawn foods and associated growth responses in Penaeus monodon. Aquaculture, v. 115, no. 1/2, p. 97-110, 2005. https://doi.org/10.1016/0044-8486(93)90361-2
Shamasunder, B. A.; Prakash, V. Physiological and functional properties of proteins from prawns Metapenaeus dobsoni. Journal of Agricultural and Food Chemistry, v. 42, p. 169-174, 1994.
Suneetha, Y.; Sreenivasula Reddy, P.; Naga Jyoti, P.; Srinivasulu Reddy, M. Proximal changes during reproduction process of the penaeid prawn Penaeus monodon. World Journal of Fish and Marine Sciences, v. 1, p. 333-337, 2009.
Weng, H. J.; Cadwallader, K. R.; Baek, H. H.; D'Abramo, L. R.; Sullivan, J. A. Manipulating the flavor of freshwater crustacea using postharvest seawater acclimation. In: Shahidi, F. C. (Ed.). Flavor and lipid chemistry of seafoods. Washington, D.C.: American Chemical Society, 1997. p. 120-130.
Wenjuan, J.; Xueliang, X. A comparative study on fatty acid composition of the prawn (Penaeus chinensis) during ovarian maturation. In: Chou, L. M.; Munto, A. D.; Lam, T. J.; Chen, T. W.; Cheong, L. K. K.; Ding, J. K.; Hooi, K. K.; Khoo, H. W.; Phang, V. P. E; Shim, K. F.; Tan, C. H. (Eds.). The Third Asian Fisheries Forum Asian Fisheries Society. Manila, The Philippines: Asian Fisheries Society, 1994. p. 701-704.
Wilson, R. P. Amino acids and Proteins. In: Halver, J. E.; Hardy, R. W. (Eds.). Fish nutrition. San Diego, Ca: Academic Press, 2002. p. 144-175.