Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 3, no. 6 (2016) Adelowo


Vol. 3, No. 6, p. 407-412 - Dec. 31, 2016


Spectrophotometric analysis of phosphate concentration in agricultural soil samples and water samples using molybdenum blue method

Funmilayo Enitan Adelowo and Samuel O. Agele

Phosphorus has a strong affinity for soil thereby increasing the rate of dissolved phosphorus that will be transported in runoff. The most important contributing factor for increasing in phosphates levels are natural and human factors such as dumping of refuse from nearby areas, use of fertilizers and human excretes. The phosphate levels in the soil and water samples (expressed in part per million) was analyzed by molybdenum blue phosphorus method using a simple analytical and UV-visible spectrophotometric method and the phosphates level was measured at 740 nm. The calibration curve was formed using concentrations of 0.5-3.0 mg/L. The samples analyzed showed high levels of phosphates which could be due to domestic, natural and agricultural factors.

Phosphates levels; Molybdenum blue phosphorus method; Calibration curve; Pollutants; UV-visible spectrophotometer.


Full text

Amponsah, D.; Etsey, G.; Nagai, H. Determination of amount of phosphate and sulphate in soil samples from university of Cape Coast Farm. International J. Sci. Tech. Res., v. 3, no. 7, p. 211-215, 2014. Available from: <;>. Accessed on: Apr. 23, 2016.

Bøtker, H. E.; Kimose, H. H.; Helligsų, P.; Nielsen, T. T. Analytical evaluation of high energy phosphate determination by high performance liquid chromatography in myocardial tissue. Journal of Molecular and Cellular Cardiology, v. 26, no. 1, p. 41-48, 1994.

Chaube, M. A.; Gupta, V. K. Spectrophotometric determination of phosphate in polluted waters by solvent extraction of molybdenum blue. Analyst., v. 108, p. 1141-1144, 1983.

Kharat S. J.; Pagar, S. D. Determination of phosphate in water samples of Nashik District Rivers by UV-visible spectroscopy. E-Journal Chem., v. 6, Suppl. 1, p. S515-S521, 2009.

McCoy, M. Goodbye phosphates. Chemical and Engineering News, v. 89, no. 4, p. 12-17, 2011. Available from: <: cen/coverstory/89/8904cover.html. Accessed on: Jun. 23, 2016.

Oladeji, S. O.; Adelowo, F. E.; Odelade, K. A. Evaluation of phosphate level in water samples (Ogbomoso Rivers) using UV-visible spectrophotometric method. Int. J. Sci. Res. Env. Sci., v. 4, no. 4, p. 102-108, 2016.

Smith, V.; Tilman, G.; Nekola, J. Eutrophication: impacts of excess nutrient in freshwater, marine and terrestrial ecosystems. Environ. Pol., v. 100, no. 1/3, p. 179-196, 1999.

Verma, N. C. b>System of technical control for cane sugar factories in India. The Sugar Technologists Association of India, 1988.

Vogel, A. A text book of quantitative chemical analysis. 5. ed. London: Longmans, 1991.

Weier T. E.; Stocking C. R.; Barbour, M. G. An introduction to plant Biology. 5. ed. Davis, California: University of California, 1973.