Thin-layer chromatographic analysis of flavonoids and total phenolics in methanolic and ethanolic extracts of Senna alata (L.) Roxb. (Fabales: Fabaceae)

Solomon Oluwole Oladeji

Abstract
Herbal medicine has proved as a major source of therapeutic agents. They have been used to combat bacterial and fungal related infections and diseases. People depend on medicinal plants such as Senna alata (L.) Roxb. (Fabales: Fabaceae) without the knowledge of the chemical composition of the plant. Therefore, there is a need to examine the bioactive components that are present in S. alata. The methanolic and ethanolic extracts were obtained using soxhlet apparatus and the concentrated extracts were purified using column chromatography. The fractions were eluted and investigated for the presence of total flavonoids and phenolics. The values of thin-layer chromatographic analysis for the leaf, flower and fruit extracts of S. alata when compared to gallic acid and quercetrin (standard) showed that the fractions contained a chemical component in the class of gallic acid and quercetrin, respectively. The presence of total phenolic and flavonoids could be responsible for the observed antifungal and antibacterial activities of the plant. It could be concluded that this plant is a natural source of antimicrobial substances of high importance and are useful in antiseptic and disinfectant formulation as well as in chemotherapy.

Keywords
Herbal medicine; Bioactive compounds; Flavonoids; Total phenolics.

DOI
10.21472/bjbs.030520

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References
Adelowo, F. E.; Oladeji, S. O. Spectrophotometric analysis of phenolic compounds in Senna alata. Am. J. Adv. Sci. Res., v. 3, p. 246-253, 2016.

Beecher, G. Overview of dietary flavonoids: nomenclature, occurrence and Intake. Journal of Nutrition, v. 133, no. 10, p. 3248S-3254S, 2003. Available from: <http://jn.nutrition.org/content/133/10/3248S.full>. Accessed in: May 6, 2016.

Biesaga, M. Influence of extraction methods on stability of flavonoids. Journal of Chromatographic A, v. 1218, no. 18, p. 2505-2512, 2011. http://dx.doi.org/10.1016/j.chroma.2011.02.059

Crozier, A.; Burns, J.; Aziz, A. A.; Stewart, A. J.; Rabiasz, H. S.; Jenkins, G. I.; Edwarss, C. A.; Lean, E. J. Antioxidant flavonols from fruits and vegetables: measurements and bioavailability. Biological Research, v. 33, 78-88, 2000. http://dx.doi.org/10.4067/S0716-97602000000200007

Farnsworth, N. R.; Bunyapraphatsara, N. Thai Medicinal Plant: recommended for Primary Health Care System. Bangkok, Thailand: Prachachon Company, 1992.

Harborne, J.; Williams, C. Advances in flavonoid research since 1992. Phytochemistry, v. 55, p. 481-504, 2000. http://dx.doi.org/10.1016/S0031-9422(00)00235-1

Indu, B.; Alan, C. Dietary flavonoids and phenolic compounds. Glasgow, United Kingdom: Faculty of Biomedical and Life Sciences, University of Glasgow, 2010.

Kumar, A.; Shukla, R.; Singh, P.; Prasad, C. S.; Dubey, N. K. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innovation Food Sci. Emerg., v. 9, no. 4, p. 575-580, 1984. http://dx.doi.org/10.1016/j.ifset.2007.12.005

Owoyale, J. A.; Olatunji, G. A.; Oguntoye, S. O. Antifungal and antibacterial activities of an alcoholic extract of Senna alata leaves. Journal of Applied Science and Environmental Management, v. 9, no. 3, p. 105-107, 2005. Available from: <http://unilorin.edu.ng/publications/olatunji/Prof. Olatunji 11.pdf>. Accessed in: May 6, 2016.

Paiva, N. L. An introduction to the biosynthesis of chemicals used in plant-microbe communication. Journal on Plant Growth Regulation, v. 19, no. 2, p. 131-143, 2000. http://dx.doi.org/10.1007/s003440000016

Rahman, M.; Yasmin, T.; Shaheen, H. L.; Shaheen, T.; Tabassam, N.; Zafar, Y. Harvesting genetic diversity in the cotton genome. National Symposium on Biotechnology for Economic Prosperity, National Commission on Biotechnology, Green Retreat Hotel, Nathiagali, Pakistan, 38, 2006.