Brazilian Journal of Biological Sciences (ISSN 2358-2731)

Home Archive v. 3, no. 5 (2016) Oladeji


Vol. 3, No. 5, p. 201-208 - Jun. 30, 2016


Screening, isolation and identification of microorganisms from petrochemical contaminated environments

Solomon Oluwole Oladeji and Kehinde Abraham Odelade

Soil is comprised of minerals, soil organic matter, water and air. The composition and proportion of these components greatly influence soil physical properties like structure and porosity. Soil bacteria and fungi play pivotal roles in various biogeochemical cycles and are responsible for the cycling of organic compounds. The view on the microbiological safety of drinking water is changing. The demand for the total absence of any pathogenic organism is no longer significant in light of the new pathogens, some of which are capable of growing in drinking water systems. This is mainly due to many pollutants that are present at much higher concentrations in groundwater than they are in most contaminated surface supplies. In order to determine the microbes, soil and water samples were collected from petrochemical industry, Eleme, Port-Harcourt, Rivers State, Nigeria, for microbiological analysis. This was carried out by the isolation, assessment and characterization of the isolated organisms. The highest bacterial counts was determined in soil sample 1 (SS1) and water sample 4 (WS4) with microbial loads of 1.48 x 106 cfu/mL and 9.40 x 105 cfu/mL and the lowest count was found in soil sample 2 (SS2) and water sample 2 (WS2) with microbial load 2.90 x 105 cfu/mL and 3.67 x 104 cfu/mL. The highest fungal counts was determined in soil sample 2 (SS2) and water sample1 (WS1) with microbial loads of 1.76 x 106 cfu/mL and 2.17 x 106 cfu/mL and the least colonies was in soil sample 1 (SS1) and water sample 2 (WS2) with microbial counts of 1.75 x 105 cfu/mL and 4.30 x 104 cfu/mL. The results present that the presence of these microbes can be linked to the prehistory of the effects or contamination of surface and underground water in this region and could leads to water-borne diseases.

Organic matter; Biogeochemical cycles; Pathogenic organisms; Pollutants, Microbes.


Full text

Ali, J. An assessment of the water quality of Ogunpa River Ibadan, Nigeria. Ibadan, Nigeria: University of Ibadan, 1991. (M.Sc. Dissertation).

APHA - American Public Health Association. Standard methods for the examination of water and wastewater. 18 ed. Washington, D. C.: Office American Public Health Association, 1992.

Arvnabh, M.; Vasishta, D.; Bhatt, N. S.; Pinal, S.; Kirit, P.; Chaitanya, P. Comparative study of physico-chemical and microbial parameters on lotic and 49 ground-waters in selected outlying areas of Central Gujarat. Journal of Chemical and Pharmaceutical Research, v. 2, no. 4, p. 174-177, 2010.

Bassey, E. E. Influence of maternal inheritance on genetic expression of yield and yield related traits in okra (Abelmoschcus esculentus (L.) Moench) in the humid tropical environment of Uyo, Southeastern Nigeria. In: Clark, E. V. (Ed.). Proceedings of African Regional Conference on Sustainable Development held in Uyo. Akwa Ibom State, 2008. v. 2, p. 50-58.

Bhatt, L. R.; Lacoul, H. D.; Lekhak, H.; Jha, P. K. Physicochemical characteristics and phytoplankton of Taudaha Lake, Kathmandu. Pollution Research, v. 18, no. 4, p. 353-358, 1999.

Burnett, R. T.; Cakmak, S.; Brook, J. R. The effect of the urban ambient air pollution mix on daily mortality rates in 11 Canadian cities. Canadian Journal of Public Health, v. 89, p. 152-156, 1998.

Edema, M. O.; Omemu, A. M.; Fapetu, O. M. Microbiology and physicochemical analysis of different sources of drinking water in Abeokuta, Nigeria. Nigerian Journal of Microbiology, v. 15, no. 1, p. 57-61, 2001.

European Union Council. Directive No. 98/44/EC of the European Parliament and of the Council of 6 July 1998, on the legal production of biotechnological inventions, 1998.

Fawole, M. O.; Oso, B. A. Laboratory Manual of Microbiology. Rev. ed. Ibadan: Spectrum Books, 2001.

Hemant, P.; Deepak, P.; Limaye, S. N. Studies on the physico-chemical status of two water bodies at Sagar City under anthropogenic influences. Advances in Applied Science Research, v. 3, no. 1, p. 31-44, 2012.

Kolade, O. A. Shallow wells as supplementary sources of water in Nasarawa, Gwon, Jos. Jos, Nigeria: Department of Geography and Planning, University of Jos, 1982. (M. Sc. Thesis).

Kumar, N. A. View on freshwater environment. Ecological Environmental and Conservation, v. 3, no. 3/4, 1997.

Mark, W. R.; Ximing, C.; Sarah, A. C. World water and food to 2025; dealing with security. Washington, D. C.: International Food Policy Research Institute, 2002.

Mills, C. W. The sociological imagination. Oxford: Oxford University Press, 2000.

Mohan, D.; Gaur, A.; Chodhary, D. Study of limnology and microbiology of Naya Talab, Jodhpur, Rajasthan. Proceeding National Symposium on Limnology, 2007. p. 64-68.

Olutiola, P. O.; Famurewa, O.; Sontang, H. G. An introduction to general microbiology: a practical approach. Geneva, Switzerland: Ca. Heidelberg verlagsanstaltund Dreuckerei GMbh., Heidelberg, Germany. 1991.

Parihar, V. L.; Sharma, M. S.; Sharma, L. L. Utility of bacteriological parameters for assessing best use and trophic status of seasonal water: a case study from Udaipur, Rjasthan. Pollution Research, v. 22, no. 2, p. 163-167, 2003.

Radha, K. R.; Dharmaraj, K.; Ranjitha, K. A comparative study on the physicochemical and bacterial analysis of drinking, bore well and sewage water in the three different places of Sivakasi. Journal of Environmental Biology, v. 28, no. 1, p. 105-108, 2007.

Roy, Y.; Kumar, R. A. A study of water quality of the rivers of Ranchi District. Industrial Journal of Environmental Protection, v. 21, no. 5, p. 398-402, 2002.

Sabata, B. C.; Nayar, M. P. River pollution in India: a case study of Ganga River. New Delhi: APH Publishing, 1995.

Tebutt, T. H. Y. Principles of quality control. Pergamon, England, 1983.

USEPA. Pesticides Industry Sales and Usage. Biological and Economic Analysis Divison. Office of Chemical Safety and Pollution Prevention. Washinghton D.C.: USEPA, 2006.

Wilson, H. R.; McFarlane, D. K.; Phillips, G. C. Spatial frequency tuning of orientation selective units estimated by oblique masking. Vision Res., v. 23, p. 873-882, 1983.