Brazilian Journal of Biological Sciences (ISSN 2358-2731)



Home Archive v. 3, no. 5 (2016) Fallah

 

Vol. 3, No. 5, p. 153-164 - Jun. 30, 2016

 

Effect of adding L-carnitine and Protexin® probiotic on performance and some blood parameters of ostrich chickens



Rozbeh Fallah and Ali Ghanbari

Abstract
This study was conducted to investigate the effects of adding L-carnitine and Protexin® probiotic on performance and some blood parameters of ostrich chickens. In this study, a total of 48 one-day old ostrich chicks randomly divided into four treatment groups. Each treatment comprised of 4 replicates and 3 animals in each replicate and reared for 8 week. The experiment was arranged in a completely randomized design. The dietary treatments were: Treatment 1 (Control): Basal diet without supplementation; Treatment 2: Protexin® probiotic with level of 1 kg/t; Treatment 3: L-carnitine 500 mg/kg diet; Treatment 4: Protexin® probiotic with level of 500 kg/t + L-carnitine 250 mg/kg diet. The results of this study indicated that diet supplementation with L-carnitine and Protexin® probiotic increased ostrich chickens weight and feed intake at 8 weeks of age in comparison to other treatment groups. The other results showed no significant differences in feed conversion ratio (FCR) among different treatments at 8 week of ages and Broilers receiving L-carnitine + Protexin® probiotic had lowest FCR compared to the other tratment groups. Furthermore, diet supplementation with L-carnitine and Protexin® probiotic increased blood glucose concentration of ostrich chickens at 8 weeks of age in comparison to other treatment groups. Futher result showed that birds received L-carnitine + Protexin® probiotic had lowest total cholesterol in compersion to other treatment groups and birds receiving L-carnitine and Protexin® probiotic had lower HDL, LDL, and VLDL concentrations in compersion control group. There were no significant effect in calcium and phosphorus concentrations between control and other treatment groups.


Keywords
L-carnitine; Protoxin; Probiotic; Growth promoters; Blood parameters; Nutritional supplements.

DOI
10.21472/bjbs.030514

Full text
PDF

References
Abdulrahim, S. M.; Haddadin, M. S. Y.; Hashlamoun, E. A. R.; Robinson, R. K. The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British Poultry Science, v. 37, no. 2, p. 341-346, 1996. http://dx.doi.org/10.1080/00071669608417865

Ahir, V. B.; Singh, K. M.; Tripathi, A. K..; Mathakiya, R. A.; Jakhesara, S. J.; Koringa, P. G.; Rank, D. N.; Jhala, M. K.; Joshi, C. G. Study of bacterial diversity in poultry gut using denaturing gradient gel electrophoresis. Iranian Journal of Applied Animal Science, v. 2, p. 227-232, 2012. Available from: <http://ijas.iaurasht.ac.ir/article_514318_02fa61deb15194b48081d038b5ab8510.pdf>. Accessed in: Apr. 25, 2016.

Alloui, M. N.; Szczurek, W.; Światkiewicz, S. The usefulness of prebiotics and probiotics in modern poultry nutrition: a review. Annal of Animal Science, v. 13, p. 17-32, 2013. Available from: <https://www.degruyter.com/dg/viewarticle.fullcontentlink:pdfeventlink/$002fj$002faoas.2013.13.issue-1$002fv10220-012-0055-x$002fv10220-012-0055-x.pdf/v10220-012-0055-x.pdf?t:ac=j$002faoas.2013.13.issue-1$002fv10220-012-0055-x$002fv10220-012-0055-x.xml>. Accessed in: Apr. 25, 2016.

Angel, C. R. A review of ratite nutrition. Animal Feed Science and Technology, v. 60, no. 3/4, p. 241-246, 1996. http://dx.doi.org/10.1016/0377-8401(96)00981-9

Ashayerizadeh, A.; Dabiri, N.; Mirzadeh, K.; Ghorbani, M. R. Effects of dietary inclusion of several biological feed additives on growth response of broiler chickens. Journal of Cell Animal and Biology, v. 5, no. 4, p. 61-65, 2011. Available from: <http://www.academicjournals.org/article/article1380271953_Ashayerizadeh et al.pdf>. Accessed in: Apr. 25, 2016.

Barham, D.; Trinder, P. An improved color reagent for the determination of blood glucose by the oxidase system analyst. The Analyst, v. 97, no. 151, p. 142-145, 1972.

Barker, D. L.; Sell, J. L. Dietary carnitine did not influence performance and carcass composition of broiler chickens and young turkey fed low- or high- fat diets. Poultry Science, v. 73, p. 281-287, 1994. http://dx.doi.org/10.3382/ps.0730281

Brand, T. S.; van der Merwe, J. P.; Salih, M.; Brand, Z. Comparison of estimates of feed energy obtained from ostriches with estimates obtained from pigs, poultry and ruminants. South African Journal of Animal Science, v. 30, p. 13-14, 2000. http://dx.doi.org/10.4314/sajas.v30i4.3888

Bremer, J. Carnitine: metabolism, functions. Physiological Reviews, v. 63, no. 4, p. 1420-1480, 1983. Available from: <http://physrev.physiology.org/content/physrev/63/4/1420.full.pdf>. Accessed in: Apr. 25, 2016.

Carbajo, E. Ostrich production to mature in more countries. World Poultry, v. 22, no. 8, p. 24-26, 2006. Available from: <http://prodb.worldpoultry.net/PageFiles/26368/001_boerderij-download-WP6553D01.pdf>. Accessed in: Apr. 25, 2016.

Cevik, A. F.; Ceylan, N. Effects of dietary L-carnitine supplementation on performance and carcass traits of broiler chickens. 3rd Ulusal Hayvan Besleme Kongresi, Adana, 2005. p. 391-396.

Cilliers, S. C.; Hayes, J. P.; Chwalibog, A.; Du Preez, J. J.; Sales, J. A comparative study between mature ostriches (Struthio camelus) and adult cockerels with respect to true and apparent metabolisable energy values for maize, barley, oats and triticale. British Poultry Science, v. 38, no. 1, p. 96-100, 1997. http://dx.doi.org/10.1080/00071669708417947

Coates, M. E.; Davies, M. K.; Kon, S. K. The effect of antibiotics on the intestinal of the chick. British Journal of Nutrition, v. 9, no. 1, p. 110-119, 1995. http://dx.doi.org/10.4314/sajas.v37i2.4029

Deeming, D. C.; Dick, A. C. K.; Ayres, L. L. Ostrich chick rearing. A stockman's guide. Oxfordshire: Ratite Conference Books, 1996.

Dibner, J. J.; Richards, J. D. Antibiotics growth promoters in agriculture: history and mode of action. Poultry Science, v. 84, no. 4, p. 634-643, 2005. http://dx.doi.org/10.1093/ps/84.4.634

Edens, F. W. An alternative for antibiotic use in poultry: probiotics. Brazilian Journal of Poultry Science, v. 5, p. 75-97, 2003. http://dx.doi.org/10.1590/S1516-635X2003000200001

EFSA. Opinion of the scientific panel on additives and products or substances used in animal feed on microorganism product Bioplus 2B®, authorized as feed additive in accordance with council. The EFSA Journal, v. 6, p. 1-4, 2003. Available from: <http://www.efsa.europa.eu/sites/default/files/scientific_output/files/main_documents/feedap_op_ej6_bioplus2b_en1,2.pdf>. Accessed in: Apr. 25, 2016.

Ferket, P. R. Manutenção da saúde intestinal em um mundo sem antibióticos. Proceedings in 13th. Ronda Latino-Americana da Alltech, Campinas, p. 26-39, 2003.

Golzar Adabi, S. H.; Cooper, R. G.; Ceylan, N.; Corduk, M. L-carnitine and its functional effects in poultry nutrition. World's Poultry Science Journal, v. 67, no. 2, p. 277-296, 2011. http://dx.doi.org/10.1017/S0043933911000304

Gomes, A. M.; Malcata, F. X. Bifidobacterium spp. and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Trends in Food Science and Technology, v. 10, no. 4/5, p. 139-157, 1999. http://dx.doi.org/10.1016/S0924-2244(99)00033-3

Gri, L .R.; Martella, M. B.; Navarro, J. L. Efficacy of probiotics in intensively reared Greater Rhea (Rhea americana) chicks. Arch Geflügelk, v. 72, no. 3, p. 136-139, 2008. Available from: <http://www.european-poultry-science.com/artikel.dll/m07-36mk_NDIxODU1Mw.PDF>. Accessed in: Apr. 25, 2016.

Gunal, M.; Yayli, G.; Kaya, O.; Karahan, N.; Sulak, O. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. International Journal Poultry Science, v. 5, no. p. 149-155, 2006. Available from: <http://www.pjbs.org/ijps/fin547.pdf>. Accessed in: Apr. 25, 2016.

Hajibabaei, A.; Shivazad, M.; Golzar Adabi, S. H.; Lavaf, A.; Eila, N. L-carnitine improves egg production in black neck ostriches. Australian Journal of Experimental Agriculture, v. 48, p. 1341-1344, 2008. http://dx.doi.org/10.1071/EA08132

Hermier, D. Lipoprotein metabolism and fattening in poultry. Journal of Nutrition, v. 127, no. 5, p. 805S-808S, 1997. Available from: <http://jn.nutrition.org/content/127/5/805S.full.pdf>. Accessed in: Apr. 25, 2016.

Huchzermeyer, F. W. Diseases of farmed crocodiles and ostriches. Rev. sci. tech. Off. int. Epiz., v. 21, no. 2, p. 265-276, 2002. Available from: <http://www.oie.int/doc/ged/D513.PDF>. Accessed in: Apr. 25, 2016.

Jin, L. Z.; Ho, Y. W.; Abdullah, N.; Jalaludin, S. Growth performance, intestinal microbial population and serum cholesterol of broiler fed diets containing Lactobacillus cultures. Journal of Poultry Science, v. 77, no. 9, p. 1259-1265, 1998. Available from: http://dx.doi.org/10.1093/ps/77.9.1259

Jin, L. Z. Probiotic in poultry: modes of action. World's Poultry Science Journal, v. 53, no. 4, p. 351-368, 1997. http://dx.doi.org/10.1079/WPS19970028

Kabir, S. M. L.; Rahman, M. M.; Rahman, M. B.; Ahmed, S. U. The dynamics of probiotics on growth performance and immune response in broilers. International Journal of Poultry Science, v. 3, p. 361-364, 2004. http://dx.doi.org/10.3923/ijps.2004.361.364

Kita, K.; Kato, S.; Yaman, M. A.; Okumura, J.; Yokota, H. Dietary L-carnitine increases plasma insulin-like growth factor-I concentration in chicks fed a diet with adequate dietary protein level. British Poultry Science, v. 43, no. 3, p. 117-121, 2002. http://dx.doi.org/10.1080/00071660120109980

Langhout, P. New additives for broiler chickens. Feed Mix, 24-27, 2000.

Leibetseder, J. Studies on effects of L-carnitine in poultry. Archiv für Tierernaehrung, v. 48, no. 1/2, p. 97-108, 1995. http://dx.doi.org/10.1080/17450399509381832

Lettner, V. F.; Zollitsch, W.; Halbmayer, E. Use of L-carnitine in the broiler ration. Bodenkultur, v. 43, p. 161-167, 1992.

Lien, T. F.; Horng, Y. M. The effect of supplementary dietary L-carnitine on the growth performance, serum components, carcass traits and enzyme activities in relation to fatty acid betaoxidation of broiler chickens. British Poultry Science, v. 42, no. 1, p. 92-95, 2001. http://dx.doi.org/10.1080/713655014

Lu, J.; Idris, U.; Harmon, B.; Hofacre, C.; Maurer, J. J; Lee, M. D. Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Applied and Environmental Microbiology, v. 69, no. 11, p. 6816-6824, 2003. http://dx.doi.org/10.1128/AEM.69.11.6816-6824.2003

Mead, G. C. Prospects for 'competitive exclusion' treatment to control salmonellas and other foodborne pathogens in poultry. The Veterinary Journal, v. 159, no. 2, p. 111-123, 2000. http://dx.doi.org/10.1053/tvjl.1999.0423

Mohan, B.; Kadirvel, R.; Natarajan, A.; Bhaskaran, M. Effect of probiotic supplementation on growth, nitrogen utilization and serum cholesterol in broilers. British Poultry Science, v. 37, no. 2, p. 395-401, 1996. http://dx.doi.org/10.1080/00071669608417870

Musa, H. H.; Chen, G. H.; Wang, K. H.; Li, B. C. M.; Mekki, D. M.; Shu, J. T.; Ju, H. P. Relation between serum cholesterol, lipoprotein concentration, and carcass characteristics in genetically lean and fat chicken breeds. Journal of Biological Science, v. 6, p. 616-620, 2006. http://dx.doi.org/10.3923/jbs.2006.616.620

National Research Council. Nutrient Requirements of Domestic Animals. Nutrient Requirements of Poultry. 9. rev. ed. Washington, DC: National Academic Press, 1994.

Nayebpor, M.; Farhomad, P.; Hashemi, A. Effects of different levels of direct fed microbial (Primalac) on growth performance and humoral immune response in broiler chickens. Journal of Animal Veterinary Advanced, v. 6, no. 11, p. 1308-1313, 2007. Available from: <http://docsdrive.com/pdfs/medwelljournals/javaa/2007/1308-1313.pdf>. Accessed in: Apr. 25, 2016.

Netherwood, T.; Gilbert, H. J.; Parker, D. S.; O'Donnell, A. G. Probiotics shown to change bacterial community structure in the avian gastrointestinal tract. Applied and Environmental Microbiology, v. 65, no. 11, p. 5134-5138, 1999. Available from: <http://aem.asm.org/content/65/11/5134.full.pdf>. Accessed in: Apr. 25, 2016.

Nouboukpo, K. E.; Tona, K.; Agbonon, A.; Gbassor, M.; Buyse, J.; Decuypere, E. Effects of L-carnitine supplementation in drinking water on layer-type chick juvenile performance. Archiv für Geflugelkunde, v. 74, no. 2, p. 116-120, 2010. Available from: <http://www.european-poultry-science.com/artikel.dll/m09-06mk_NDIxOTU1OA.PDF>. Accessed in: Apr. 25, 2016.

Oakley, B. B.; Lillehoj, H. S.; Kogut, M. H.; Kim, W. K.; Maurer, J. J.; Pedroso, A.; Lee, M. D.; Collett, S. R.; Johnson, T. J.; Cox, N. A. The chicken gastrointestinal microbiome. FEMS Microbiology Letters, v. 360, no. 2, p. 100-112, 2014. http://dx.doi.org/10.1111/1574-6968.12608

Onifade, A. A. Growth performance, carcass characteristics, organ measurements and hematology of broiler chickens fed a high fiber diet supplemented with antibiotics or dietary yeast. Die Nahrung, v. 41, no. 6, p. 370-374, 1997. http://dx.doi.org/10.1002/food.19970410612

O'Sullivan, G. C.; Kelly, P.; O'Halloran, S.; Collins, C.; Collins, J. K.; Dunne, C.; Shanahan, F. Probiotics: an emerging therapy. Current Pharmaceutical Design, v. 11, no. 1, p. 3-10, 2005. http://dx.doi.org/10.2174/1381612053382368

Paryad, A.; Mahmoudi, M. Effect of different levels of supplemental yeast (Saccharomyces cerevisiae) on performance, blood constituents and carcass characteristics of broiler chicks. African Journal of Agriculture Research, v. 3, no. 12, p. 835-842, 2008. Available from: <http://www.academicjournals.org/journal/AJAR/article-full-text-pdf/AD3847E38476>. Accessed in: Apr. 25, 2016.

Rabie, M. H.; Szilágyi, M. Effects of L-carnitine supplementation of diets differing in energy levels on performance, abdominal fat content and yield and composition of edible meat of broilers. British Journal of Nutrition, v. 80, no. 4, p. 391-400, 1998. http://dx.doi.org/10.1017/S0007114598001457

Rebouche, C. J. L-carnitine function and requirements during the life cycle. Journal of the Federation of American Societies for Experimental Biology, v. 6, no. 15, p. 3379-3386, 1992. Available from: <http://www.fasebj.org/content/6/15/3379.long>. Accessed in: Apr. 25, 2016.

Rebouche, C. J. Ascorbic acid and carnitine biosynthesis. American Clinical of Nutrition, v. 54, no. 6, p. 1147S-1152S, 1991.

Rezaei, H.; Khorshidi, K. J.; Fallah, R. The effect of feeding Primalac probiotics on growth performance and blood parameters of ostriches. Malaysian Journal of Animal Science, v. 16, no. 1, p. 79-86, 2013. Available from: <http://www.msap.my/mjas16/8-Hasan MJAS 16(1) 2013.pdf>. Accessed in: Apr. 25, 2016.

Rodehutscord, M.; Timmler, R.; Dieckmann, A. Effect of L-carnitine supplementation on utilisation of energy and protein in broiler chicken fed different dietary fat levels. Archives of Animal Nutrition, v. 56, p. 431-441, 2002. http://dx.doi.org/10.1080/00039420215637

Sales, J. Digestive physiology and nutrition of ratites. Avian Poultry Biology Review, v. 17, no. 3, p. 41-55, 2006. http://dx.doi.org/10.3184/147020606783437912

SAS - Statistical Analysis System. SAS User Guide: Release 9.2. Cary NC, USA: SAS Institute Inc., 2000.

Sayed, A. N.; Shoeib, H. K.; Nayeib, H. A. Effect of dietary L-carnitine on performance of broiler chickens fed on different levels of fat. Assiut Veterinary Medical Journal, v. 45, p. 37-47, 2001.

Schrezenmeir, J.; De Vrese, M. Probiotics, prebiotics, and synbiotics: approaching a definition. American Journal of Clinical Nutrition, v. 73, no. 2, p. 361S-364S, 2001. Available from: <http://ajcn.nutrition.org/content/73/2/361s.full.pdf>. Accessed in: Apr. 25, 2016.

Shivaprasad, H. L. Neonatal mortality in ostriches: an overview of possible causes. Proceedings of the Annual Conference of the European Committee Association of Avian Veterinarians, San Diego, California, p.283-293, 1993.

Thomas, L. Clinical laboratory diagnostics: use and assessment of clinical laboratory results. Frankfurt, Germany: TH-Books Verlagsgeselschaft, 1998. p. 192-202.

Tournuyt, J. R. Probiotics. Proceedings of the 35th Reunião Annual of the Sociedade Brasileira de Zootecnia, Botucatu, São Paulo, Brasil, p. 179-199, 1998. Available from: <http://www.sbz.org.br/reuniaoanual/anais/arq_reuniao_anual/sbz1998.rar>. Accessed in: Apr. 25, 2016.

Waite, D. W.; Taylor, M. W. Characterizing the avian gut microbiota: membership, driving influences, and potential function. Frontiers in Microbiology, v. 5, Article 223, 12 p., 2014. http://dx.doi.org/10.3389/fmicb.2014.00223

Xu, Z. R.; Wang, M. Q.; Mao, H. X.; Zhan, X. A.; Hu, C. H. Effects of L-carnitine in growth performance, carcass composition, and metabolism of lipids in male broilers. Poultry Science, v. 82, p. 408-413, 2003. Available from: <http://ps.oxfordjournals.org/content/82/3/408.full.pdf>. Accessed in: Apr. 25, 2016.

Xu, Q.; Liu, J.; Zhang, Y.; Bai, Y.; Deng, Z.; Guo, X.; Zhao, P.; Song, X.; Chu, X. Application of microbial ecological agents in ostrich brooding. Acta Agriculturae Boreali-Occidentalis Sinica, v. 5, p. 13-19, 2010.

Yeo, J.; Kim, K.I. Effect of feeding diets containing an antibiotic, a probiotics or yucca extract on growth and intestinal urease activity in broiler chicks. Poultry Science, v. 76, no. 2, p. 381-385, 1997. http://dx.doi.org/10.1093/ps/76.2.381