Vol. 3, No. 5, p. 27-35 - Jun. 30, 2016
Phytochemistry and microscopy of Tapinanthus dodoneifolius (DC) (Danser) (Santalales: Loranthaceae) (African mistletoes) from guava, rubber and orange host trees
MacDonald Idu



Abstract
Qualitative and quantitative phytochemistry, micro and macro-elements and microscopy of Tapinanthus dodoneifolius (DC) (Danser) (Santalales: Loranthaceae) collected from guava (Psidum guajava L.), rubber (Hivea brasilensis Müll. Arg.) and orange (Citrus sinensis (L) Osbeck.) trees was carried out. This was done with the purpose of comparing their pharmacological and biological contents. Qualitative and quantitative phytochemical screenings were done using described methods. Microscopic study was done with the aid of a light microscope and photomicrographs were taken using a 14-mega pixel Sony cyber-shot. Result of phytochemical analysis showed the presence of oxalate, phytate, saponin, alkaloid, glycoside and tannin in African mistletoes. These phytochemicals were present in proportions that varied with their hosts. The microscopic observation showed no significant differences in the internal structures of mistletoes from the three host trees. From this present study, more differences were observed in the phytochemistry than the anatomy of the mistletoes understudied.
Keywords
African mistletoes; Phytochemistry; Microscopy; Micro and macro-elements.
DOI
10.21472/bjbs.030503
Full text
PDF
References
Adodo, A. Nature power: A Christian Approach to Herbal medicine. 3. ed. Ewu, Edo State: PAX Herbal Clinic and Research
Laboratories, 2012.
Arima, H.; Danno, G. Isolation of antimicrobial compound from guava (Psidum guajava L.). Biotech Biochem.,
v. 66, p. 1727-1730, 2002.
Ataman, J. E.; Idu, M.; Odia, E. A.; Omogbai, E. K. I.; Amaechina, F.; Akhigbe, A. O.; Ebite, L. E. Histopathologic effects
of Stachytarpheta jamaicensis (L.) Vahl. on wistar rats. Pakistan J. Biol. Sci., v. 9, p. 477-482, 2006.
Bhandari, J. B.; Mukhopadhyay, R. Morphological anatomical and phytochemical observations on two species of Vittaria smith.
In: Maheshwari, J. K.; Jain, A. P. (Eds.). Recent researches in plant anatomy and morphology. Jodhpur: Scientific
Publishers (India), 2001.
Brito-Arias, M. Synthesis and characterization of glycoside. New York: Springer, 2007.
Burger, A. M.; Mengs, U.; Schuler, J. B.; Fiebig, H. H. Anticancer activity of an aqueous mistletoe extract (AME) in syngeneic
murine tumor models. Anticancer Res., v. 21, p. 1965-1968, 2001.
Cetin, E. S.; Ozcelik, N. Apoptotic mechanism of mistletoe (Viscum album) extract used in the treatment of cancer:
review. Turk Klin Tip Bilim., v. 27, p. 533-539, 2007.
Cunniff, P. (Ed.). Official methods of analysis of Association of Official Analytical Chemist International. 18. ed.
Mariland, USA: A.O.A.C., 2005.
Deeni, Y. Y.; Sadiq, N. M. Antimicrobial properties and phytochemical constituents of the leaves of African mistletoe
(Tapinanthus dodoneifolius (DC) (Danser) (Loranthaceae): an ethnomedicinal plant of Hausaland, Northern Nigeria.
J. Ethnopharmacol., v. 83, p. 235-240, 2002.
Edeoga, H. O.; Eboka, A. U. Morphology of the leaf epidermis and systematic in some Dissotis Benth species
(Melastomataceae). Global J. Pure and Applied Sci., v. 6, n. 3, p. 371-374, 2000.
Edeoga, H. O.; Ikem, C. I. Comparative morphology of leaf epidermis in three species of Boerhevia L.
J. Econ. Tax. Bot., v. 19, p. 197-205, 2001.
Ekhaise, F. O.; Ofoezie, V. G.; Enobakhare, D. A. Antibacterial properties and preliminary phytochemical analysis of
methanolic extract of mistletoe (Tapinanthus bangwensis). Bayero J. Pure Appl. Sci., v. 3, p. 65-68, 2010.
Greiner, R.; Konietzny, U.; Jany, K.-D. Phytate - an undesirable constituent of plant-based foods? Journal für
Ernährungsmedizin, v. 8, p. 18-28, 2006.
Gupta, G.; Kazmi, I.; Afzal, M.; Rahman, M.; Saleem, S.; Ashraf, M. S.; Khusroo, M. J.; Nazeer, K.; Ahmed, S.; Mujeeb, M.;
Ahmed, Z.; Anwar, F. Sedative, antiepileptic and antipsychotic effects of Viscum album L. (Loranthaceae) in mice
and rats. J. Ethnopharmacol., v. 141, p. 810-816, 2012.
Hong, C. E.; Lyu, S. Y. The antimutagenic effect of mistletoe lectin (Viscum album L. var. coloratum agglutinin).
Phytother. Res., v. 26, p. 787-790, 2012.
Ibrahim, A. L.; Ayodele, A. E.; Okhale, S. E.; Jegede, I. A.; Kunle, O. F. The taxonomic significance of the micro-morphology/phytochemistry
of Agelanthus dodoneifolius in relation to its hosts. Nig. J. Bot., v. 22, p. 89-101, 2009.
Idu, M.; Erhabor, J.; Timothy, O.; Ovuakporie-Uvo, O. Market survey and heavy metal screening of selected medicinal plants sold
in some markets in Benin City, Nigeria. BEST: Intl. J. Humanities, Arts, Med. Sci., v. 2, p. 7-16, 2014.
Idu, M.; Gill, L. S.; Omonhinmin, C. A.; Ejale, A. Ethnomedicinal uses of trees among Bachama Tribe of Adamawa State, Nigeria.
Indian J. Trad. Knowl., v. 5, p. 273-278, 2006.
Idu, M.; Oshomoh, E. O.; Ovuakporie-Uvo, P. O. Phytochemistry and antimicrobial properties of Chlorophora excelsa, Cedrela
odorata and Tectona grandis. Topclass J. Herbal Med., v. 2, p. 248-253.
Jenab, M.; Thompson, L. U. Phytic acid in wheat bran affects colon morphology, cell differentiation and apoptosis.
Carcinogenesis, v. 21, p. 1547-1552, 2000.
Judd, W. S.; Campbell, C. S.; Kellog, E. A.; Stevens, P. F.; Donaglue, M. J. Plant systematics: a phylogenetic approach.
2. ed. Sunderland: Sinaeur Associates, 2002. Availabe from: <http://donoghuelab.yale.edu/sites/default/files/122_judd_2002_2nded.pdf>.
Accessed in: Jan. 23, 2016.
Kumari, M.; Jain, S. Tannins: An Antinutrient with positive effect to manage Diabetes. Res. J. Recent Sci., v. 1, p. 70-73, 2012.
Lavastre, V.; Cavalli, H.; Ratthe, C.; Girard, D. Anti-inflammatory effect of Viscum album agglutinin-I (VAA-I): induction of apoptosis
in activated neutrophils and inhibition of lipopolysaccharide-induced neutrophilic inflammation in vivo. Clin. Exp. Immunol.,
v. 137, p. 271-278, 2004.
Luczkiewicz, M.; Cisowski, W.; Kaiser, P., Ochocka, R.; Piotrowski, A. Comparative analysis of phenolic acids in mistletoe plants from various
hosts. Acta. Pol. Pharm., v. 58, p. 373-379, 2001.
Malik, I. O.; Babiker, E. E.; Yousif, N. E.; El Tinay, A. H. In vitro availability of minerals of some tropical and citrus fruits as
influenced by anti-nutritional factors. Food/Nahrung., v. 48, p.65-68. 2004.
Martin, A. P.; Salgueiro, L.-R.; Conclaves, M. J.; Vila, R.; Tomi, A. T.; Cunha, A. P.; Canigueral, S.; Casanova, J. Antimicrobial activity
and chemical composition of bark oil of Croton stellulifier. Planta Medica, v. 66, p. 647-652, 2002.
Obadoni, B. O.; Ochuko, P. O. Phytochemical studies and comparative efficacy of the crude extracts of some homostatic plants in Edo and Delta
States of Nigeria. Global J. Pure Applied Sci., v. 8, p. 203-208, 2001.
Ofem, O. E.; Eno, A. E.; Imoru, J.; Nkanu, E.; Unoh, F.; Bu, J. D. Effect of crude aqueous leaf extract of Viscum album (mistletoe)
in hypertensive rats. Indian J. Pharmacol., v. 39, p. 15-19, 2007.
Ogunleye, D. S.; Ibitoye, S. F. Studies of antimicrobial activity and chemical constituents of Ximenia americana. Tropical
J. Pharm Res., v. 2, p. 239-241, 2003.
Okwu, D. E. Phytochemicals, vitamins and mineral contents of two Nigeria medicinal plants. J. Mol. Med. Adv. Sci., v. 1,
p. 378-381, 2005.
Olapade, E. O. The herbs for good health: the 50th Anniversary Lecture of the University of Ibadan. Ibadan, Nigeria: NARL
Specilist Clinic, 2002.
Orhan, D. D.; Aslan, M.; Sendogdu, N.; Ergun, F.; Yesilada, E. Evaluation of the hypoglycemic effect and antioxidant activity of
three Viscum album subspecies (European mistletoe) in streptozotocin-diabetic rats. J. Ethnopharmacol., v. 98,
p. 95-102, 2005.
Rahman, M. M.; Kawamura, O. Oxalate accumulation in forage plants: some agronomic, climatic and genetic aspects. Asian-Aust J. Anim.
Sci., v. 24, p. 439-448, 2011.
Rani, S. S.; Murthy, K. S. R.; Pullaiah, T. Dye yielding Plants of Andhra Pradesh, India. J. Economic Taxonomic Bot., v. 26,
p. 739-749, 2002.
Sabova, L.; Pilatova, M.; Szilagyi, K.; Sabo, R.; Mojzis, J. Cytotoxic effect of mistletoe (Viscum album L.) extract on jurkat
cells and its interaction with doxorubicin. Phytother. Res., v. 24, p. 365-368, 2010.
Shahaboddin, M. E.; Pouramir, M.; Moghadamnia, A. A.; Lakzaei, M.; Mirhashemi, S. M.; Motallebi, M. Antihyperglycemic and antioxidant
activity of Viscum album extract. African J. Pharm. Pharmacol., v. 5, p. 432-436, 2011.
Shaw, W. The roles of oxalates in autism and chronic disorders. The Weston A, Price Foundation for wise traditions in food, farming and
the healing arts. U.S.A., 2010. Available from: <http://www.sott.net/articles/show/215309-The-Role-ofOxalates-in-Autism-and-chronic-Disorders>.
Accessed in: Jan. 23, 2016.
Sofowora, E. A. Medicinal plants and traditional medicine in Africa. Nigeria: John Wiley and Sons Ltd., 2008.
Stephen, U. A.; Abiodun, F.; Osahon, O.; Ewaen, E. Phytochemicalanalysis and antibacterial activity of Khaya grandifolia stem bark.
J. Biol. Sci., v. 9, p. 63-67, 2009.
Tenorio-Lopez, F. A.; Del Valle, L.; Gonzalez, A.; Pastelin, G. Vasodilator activity of the aqueous extract of Viscum album.
Fitoterapia, v. 76, p. 204-209, 2005.
Tenorio-Lopez, F. A.; Mondragon, L. D.; Olvera, G. Z.; Narvaez, J. C. T.; Pastelin, G. Viscum album aqueous extract induces NOS-2
and NOS-3 over-expression in Guinea pig hearts. Nat. Prod. Res., v. 20, p. 1176-1182, 2006.
Uthman, G. S.; Bika, S. N.; Timothy, S. Y. Anticonvulsant screening of the methanolic leaf extract of Tapinanthus dodoneifolius Danser
(Loranthaceae) in pentylenetetrazole induced seizure model in albino rats. International Journal of Pharma and Bio Sciences, v. 6,
p. 245-250, 2015.
Vicas, S. I.; Rugina, D.; Leopold, L.; Pintea, A.; Socaciu, C. HPLC fingerprint of bioactive compounds and antioxidant activities of Viscum
album from different host trees. Not Bot Horti Agrobot Cluj-Napoca, v. 39, p. 48-57, 2011.
Vucenik, I.; Shamsuddin, A. M. Cancer inhibition by inositol hexaphosphate (IP6) and inositol: from laboratory to clinic. J. Nutr.,
v. 133, p. 3778S-3784S, 2003.