Vol. 2, No. 4, p. 221-233 - Dec. 31, 2015
Plant growth promoter and biocontrol mechanism of endophytic fungi Botrytis sp.
G. Senthilmurugan@viji and S. Sekar
In this study, an endophytic fungus Botrytis sp isolated from healthy aerial root of Ficus benghalensis (Indian Banyan). It was tested for antagonistic and volatile activity against plant pathogens (Fusarium sp and Diplocarpon rosae). Moreover we have analyzed that endophytic fungal culture filtrate for herbicidal activity, seed germination test, pot culture experiment and estimation of chlorophyll and protein. Endophytic fungi Botrytis sp has showed significant inhibition in antagonistic and volatile activity against Diplocarpon rosae but slight antagonism and volatile activity against Fusarium sp however, endophytic fungi secondary metabolites showed no wilt symptom in herbicidal activity against Parthenium hysterophorous weed. The seed germination test analysis of endophytic fungi culture filtrate was found to be efficient enhancing germination of Sorghum bicolor and Vigna unguiculata seeds. In pot culture experiment also, Sorghum bicolor plants were treated with endophytic fungi culture filtrate promoted growth, when compared to control and also showed higher chlorophyll content at 20% treatment and protein was found to be higher in all concentrations.
Aerial root, Botrytis sp, Endophytic fungi, Ficus benghalensis.
Amirita, A.; Sindhu, P.; Swetha, J.; Vasanthi, N. S.; Kannan, K. P. Enumeration of endophytic fungi from medicinal plants and screening of extracellular enzymes. World Journal of Science and Technology, v. 2, n. 2, p. 13-19, 2012.
Arnold, A. E. Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biology Reviews, v. 21, p. 51-66, 2007.
Arnon, D. I. Copper enzymes in isolated choloroplasts, polyphenol oxidase in Beta vulgaris. Plant. Physiol., v. 24, p. 4-15, 1949.
Dennis, C.; Webstar, J. Antagonistic properties of species group of Trichoderma. II. Production of volatile antibiotics. Trans. Brit. Mycol. Soc., v. 57, p. 41-48, 1971.
Huang, Y.; Wang, J.; Li, G.; Zheng, Z.; Su, W. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalotaxus fortune and Torreya grandis. FEMS Immunol. Med. Microbiol., v. 31, p. 163-167, 2001.
Huang, W. Y.; Cai, Y. Z.; Hyde, K. D.; Corke, H.; Sun, M. Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Diversity, v. 33, p. 61-75, 2008.
Jalgaonwalal, R. E.; Mohite, B. V., Mahajan, R. T. Evaluation of endophytes for their antimicrobial activity. Afr. J. Biotechnol., v. 10, n. 1, p. 103-107, 2011.
Khan, S. A.; Hamayun, M.; Khan, A. L.; Lee, I.-J.; Shinwari, Z. K.; Kim, J.-G. Isolation of plant growth promoting endophytic fungi from dicots inhabiting costal sand dunes of Korea. Pak. J. Bot., v. 44, n 4, p. 1453-1460, 2012.
Krishnamurthy, Y. L.; Hemalatha, T. V. Isolation of endophytic fungi from some grasses. J. Mycol. Plt. Pathol., v. 33, n. 2, p. 305-306, 2003.
Krishnamurthy, Y. L.; Naik, S. B.; Jayaram, S. Fungal communities in herbaceous medicinal plants from the Malnad Region', Southern India. Microbes Environ., v. 23, p. 24-28, 2008.
Lahlali, R.; Hijri., M. Screening, identification and evaluation of potential biocontrol fungal endophytes against Rhizoctonia solani AG3 on potato plants. FEMS Microbiol. Lett., v. 311, n. 2, p. 152-159, 2010.
Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with folin phenol reagent. J. Bio. Chem., v. 193, p. 265-275, 1951.
Morton, D. T.; Strobe, W. H. Antagonistic and stimulatory effects of soil microorganism upon Sclerotium. Phytopatho., v. 45, p. 417-420, 1995.
Saikkonen, K.; Wali, P.; Helander, M.; Faeth, S. H. Evolution of endophyte-plant symbioses. Trends in Plant Science, v. 9, p. 275-280, 2004.
Strobel, G.; Daisy, B. Bioprospecting for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev., v. 67, p. 491-502, 2003.
Suryanarayanan, T. S.; Vijayakrishna, D. Fungal endophytes of aerial roots of Ficus benghalensis. Fungal Diversity, v. 8, p. 155-161, 2001.
Vikrant, P.; Verma, K. K.; Rajak, R. C.; Pandey, A. K. Characterization of a phytotoxin from Phoma herbarum for management of Parthenium hysterophorus L. J. Phytol., v. 154, p. 461-468, 2006.
Worpong, J.; Strobel, G. A.; Ford, E. J.; Li, J. Y., Baird, G.; Hess, W. M. Muscodor albus gen. et sp. nov., an endophyte from Cinnamomum zeylanicum. Mycotaxon, v. 79, p. 67-79, 2001.