Brazilian Journal of Biological Sciences

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Vol. 1, No. 1, p. 28-34 - Jun. 30, 2014


Chronic toxicity bioassay with ampularid snail Pomacea lineata (Spix 1824) (Mollusca: Prosobranchia)

Luiz Eduardo Lima Melo, Ronilson José da Paz, José Etham de Lucena Barbosa, Takako Watanabe, Judith Font Batalla and Robert Anthony Coler


Pomacea lineata (Spix, 1824), an extremely ubiquitous snail and pest to rice farmers throughout the orient, holds promise as a valuable resource for monitoring water quality in Northeast Brazil. In this paper, we present data to demonstrate its capacity as a test animal to consistently respond to low level herbicide concentrations (Paraquat and Roundup). Growth data to assess chronic toxicity, were generated in only 96 hours. Applying Duncan's test we were able to tentatively approximate a 96 and 192 hour no observed effective concentration (NOEC) and lowest observed effective concentration (LOEC) for Roundup of 1.0 and 2.0 and 0.5 and 1.0 mg/L, respectively (no acute toxicity was observed during 15 days of exposure.) No such estimates could be attempted with Paraquat, however, because every concentration tested was significantly different from the control percent increase in growth even at the lowest concentration tested (0.005 mg/L) there was an increase in growth that was significantly greater than control levels suggesting a hormetic effect.

Biomonitoring, herbicide pollution, ecotoxicity.

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Aragão, R. M.; Xavier, B.; Coler, R.; Watanabe, T. A preliminary study of respiration and excretion rates in prawn Macrobrachium amazonicum when exposed to varied flow regimes and salinities. Rev. Nordestina Biol., v. 12, p. 1-8, 1998.

Barbosa, J. E. L.; Watanabe, T.; Coler, R. A.; Paz, R. J. Biota toxicity to herbicide Paraquat in Brazilian Northeast littoral reservoirs: need for a biomonitoring program for watershed management. Brazilian Journal of Biological Sciences, v. 1, p. 21-27, 2014.

Batalla, J. F. Efeito do herbicida Paraquat sobre o gastrópode Pomacea lineata (Spix, 1827) (Ampullariidae, Prosobranchia): Bioensaios em laboratório. João Pessoa: Universidade Federal da Paraíba, 1997. (Masters Thesis).

Buikema, A. L.; Geiger, J. G.; Lee, D. R. Daphnia toxicity tests. Aquatic Invertebrate Bioassays, v. 3, p. 48-69, 1980.

Bustos-Obregon, E.; Vargas, Á. Chronic toxicity bioassay with populations of the crustacean Artemia salina exposed to the organophosphate diazinon. Biol. Res., v. 43, p. 357-362, 2010.

Calabrese, E. J.; Baldwin, L. Possible examples of chemical hormesis in a previously published study. J. Appl. Toxicol., v. 13, p. 169-172, 1993.

Coler, R. A.; Coler, R. R.; Felizardo, E. K. G.; Watanabe, T. Applying weight gain in Pomacea lineata (Spix, 1824) (Mollusca: Prosobranchia) as a measure of herbicide toxicity. Braz. J. Biol., v. 65, p. 617-623, 2005.

Coler, R. A.; Watanabe, T.; Xavier, B. F.; Paz, R. J. A preliminary report on the application of Macrobrachium amazonicum Heller, 1862 (Decapoda: Palaemonidae) as a biomarker. Hydrobiologia, v. 412, p.119-121, 1999.

Cuhra, M.; Traavik, T.; Bøhn, T. Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna. Ecotoxicology, v. 22, p. 251262, 2013.

Eaton, A. D.; Clesceri, L. S.; Greenberg, A. E. (Ed.). Standard Methods for the Examination of Water & Wastewater. New York: American Public Health Association Publications, 1995.

Gadelha, C. L. M.; Filgueira, H. J. A.; Campos, L. F.; Quinino, U. C. M. O uso de agrotóxicos nas áreas irrigadas da Bacia do Rio Gramame no Estado da Paraíba. Anais do 21º Congresso Brasileiro de Engenharia Sanitária e Ambiental, Rio de Janeiro, 2001. 6 p.

Medeiros, V. M.; Watanabe, T.; Coler, R. R.; Coler, R. A. Development of methods to assess the impact of herbicide use on the benthos of littoral impoundments in Northeast Brazil. Journal of Aquatic Ecosystem Stress and Recovery, v. 9, p. 67-71, 2001.

Melo, L. E. L.; Coler, R. A.; Watanabe, T.; Batalla, J. F. Developing the gastropod Pomacea lineata (Spix, 1827) as a toxicity test organism. Hydrobiologia, v. 429, p. 73-78, 2000.

Rand, G. M.; Petrocelli, S. R. (Ed.). Fundamentals of Aquatic Toxicology. Washington: MacGraw Hill, 1995.

Rocha, T. R. P.; Watanabe, T.; Sassi, R.; Coler, R. A. The photosynthetic/respiratory response of a periphytic population (Selenastrum capricornutum) to Paraquat as a biomarker. Acta Limnol. Brasil., v. 10, p. 131-136, 1998.

Sprague, J. B. Factors that modify toxicity. In: Rand, G. M.; Petrocelli, S. R. (Ed.). Fundamentals of Aquatic Toxicology. Washington: MacGraw Hill, 1995. p. 125-167.

Tabosa, W. A. F. Bioensaios com Lemna minor (Linnaeus, 1753), um estudo da toxicidade de um efluente industrial no Rio Gramame. João Pessoa: Universidade Federal da Paraíba, 2000. (Masters Thesis).

USEPA - United States Environmental Protection Agency. R.E.D. Facts: Glysophate. Washington DC: USEPA Offices of Prevention, Pesticides And Toxic Substances, 1993.

USEPA - United States Environmental Protection Agency. R.E.D. Facts: Paraquat Dichloride. Washington DC: USEPA Offices of Prevention, Pesticides And Toxic Substances, 1997.

Watanabe T.; Coler, R. A.; Paz, R. J. The implementation of a regional biomonitoring program in Northeast Brazil. Aquatic Ecosystem Health and Management, v. 2. p. 187-189, 1999.